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Article Citation - Scopus: 1Surface Patterning of Poly(ε-Caprolactone) Scaffolds by Electrospinning for Monitoring Cell Biomass Behavior(Springer, 2022) Albayrak, Deniz; Sasmazel, Hilal TurkogluThe aim of this work was to produce three-dimensional fibrous surface patterns of poly(epsilon-caprolactone) (PCL), onto two-dimensional smooth solvent cast PCL surfaces with an electrospinning method by using a mask/stencil for monitoring cell biomass behavior. The characterizations of produced scaffolds were done by thickness measurements, scanning electron microscopy (SEM) analyses, contact angle (CA) measurements, Fourier-transform infrared spectroscopy (FTIR), and mechanical tests. According to SEM micrographs, all of the electrospun scaffold surfaces exhibited bead-free and uniform morphology while solvent cast surfaces were smooth and nonporous. CA measurements revealed that the solvent cast surfaces had moderate hydrophilicity (similar to 60 degrees) while electrospun regions had a more hydrophobic character (similar to 110 degrees for fully electrospun surfaces and similar to 85 degrees for electrospun patterns). Mechanical testing showed the produced scaffolds had a brittle character. Moreover, cell culture studies were performed with mouse fibroblast (L929) cells for 7 days period, and cell attachment assay, MTT assay, fluorescence, and SEM analyses were done. Cell culture studies indicated that the solvent cast and electrospun patterns have different characteristics for cell behavior. Thus, cell movement, attachment, and proliferation can be directed and monitored by obtaining different surface topographies in a single substrate surface. Based on the results of this study, it was found that patterns consisting of polymeric nanofiber structures can also be created directly by the electrospinning method.Book Part Poly(ε-caprolactone)/Chitosan Nanostructures for Cell Cultivation(Springer, 2020) Turkoglu Sasmazel,H.Hybridization of synthetic poly (ε-caprolactone) (PCL) and natural chitosan polymers to develop PCL/chitosan core-shell nanostructures for cell cultivation was aimed in this study. Coaxial electrospinning method was used for the fabrication of the nanostructures. The characterizations of the samples were done by X-ray photoelectron spectroscopy (XPS) analyses and mechanical tests. XPS analysis of the PCL/chitosan core-shell structures exhibited the characteristic peaks of PCL and chitosan polymers. The cell culture studies, MTT assay and Confocal Laser Scanning Microscopy (CLSM), carried out with L929 ATCC CCL-1 mouse fibroblast cell line, proved the biocompatibility of all materials. The cell viability on the hybrid nanostructures was ~two times better then on tissue culture polystyrene (TCPS) because of its three dimensional (3D) extracellular matrix (ECM)-like structure compared to 2D flat surface of commercially cell compatible TCPS. The performance was ~two times and ~ten times better compared to single PCL and single chitosan, respectively, even though both fabricated similarly by electrospinning as non-woven fibrous structures, because were either too hydrophobic or too hydrophilic to maintain cell attachment points. © Springer Nature B.V. 2020.
