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Article Citation - WoS: 3Citation - Scopus: 3Characterization of Mesenchymal Stem Cells in Mucolipidosis Type Ii (i-Cell Disease)(Tubitak Scientific & Technological Research Council Turkey, 2019) Köse, Sevil; Kaya, Fatima Aerts; Kuşkonmaz, Bülent Barış; Çetinkaya, Duygu Uçkan; Aerts Kaya, Fatima; Uckan Cetinkaya, DuyguMucolipidosis type II (ML-II, I-cell disease) is a fatal inherited lysosomal storage disease caused by a deficiency of theenzyme N-acetylglucosamine-1-phosphotransferase. A characteristic skeletal phenotype is one of the many clinical manifestationsof ML-II. Since the mechanisms underlying these skeletal defects in ML-II are not completely understood, we hypothesized that adefect in osteogenic differentiation of ML-II bone marrow mesenchymal stem cells (BM-MSCs) might be responsible for this skeletalphenotype. Here, we assessed and characterized the cellular phenotype of BM-MSCs from a ML-II patient before (BBMT) and afterBM transplantation (ABMT), and we compared the results with BM-MSCs from a carrier and a healthy donor. Morphologically, wedid not observe differences in ML-II BBMT and ABMT or carrier MSCs in terms of size or granularity. Osteogenic differentiation wasnot markedly affected by disease or carrier status. Adipogenic differentiation was increased in BBMT ML-II MSCs, but chondrogenicdifferentiation was decreased in both BBMT and ABMT ML-II MSCs. Immunophenotypically no significant differences were observedbetween the samples. Interestingly, the proliferative capacity of BBMT and ABMT ML-II MSCs was increased in comparison to MSCsfrom age-matched healthy donors. These data suggest that MSCs are not likely to cause the skeletal phenotype observed in ML-II, butthey may contribute to the pathogenesis of ML-II as a result of lysosomal storage-induced pathology.Article Citation - WoS: 3Citation - Scopus: 4Identification of Bacterial Diversity of Bee Collected Pollen and Bee Bread Microbiota by Metagenomic Analysis(Aves, 2022) Arserim Ucar, Dilhun Keriman; Yurt, Mediha Nur Zafer; Tasbasi, Behiye Busra; Acar, Elif Esma; Yegin, Zeynep; Ozalp, Veli Cengiz; Sudagidan, Mert; Uçar, Dilhun Keriman Arserim; Ozalp, Cengiz; Arserim-uçar, Dılhun KerimanThis study investigated the bacterial diversities of bee-collected pollen and bee bread of Apis mellifera in Turkey. The bacterial community structure of 14 bee pollen from Bingol, Konya, and Hakkari and 11 bee bread samples from Bingol were studied using 16 S rRNA amplicon sequencing and metagenomic analysis. The dominant bacterial phylum in pollen and bee bread samples was Firmicutes, followed by Proteobacteria. In pollen and bee bread samples, Bacillaceae, Clostridiaceae, Enterococcaceae, and Enterobacteriaceae were identified as dominant bacterial families. At the genus level, Bacillus, Clostridium sensu stricto, and Enterococcus were dominant bacteria in both pollen and bee bread samples. The most abundant species was Clostridium perfringens in both pollen and bee bread samples. Escherichia vulneris, Enterococcus faecalis, Bacillus cereus, Enterococcus casseliflavus, and Cronobacter malonaticus were identified with high reads in pollen samples. In bee bread samples, E. faecalis, Clostridium bifermentans, and Pantoea calida were abundant bacterial species. Alpha diversity showed that pol-3 sample had the highest diversity. Beta-diversity plots separated the pollen samples into four main groups and bee bread samples into three main groups. Our results indicated that the culture-independent metagenomic analysis will be a valuable tool for determining the microbial diversity of bee products produced in Bingol-Turkey one of the important centers of apiculture.Review Çikolata ve Sağlık Beyanları(2018) Cömert, Tuğba Küçükkasap; Merdol, Türkan KutluayÇikolata, bileşimindeki kakao oranı ve yapımında kullanılan maddelere göre bitter çikolata, sütlü çikolata vb. olarakadlandırılır. Kakao oranı ile en zengin olan bitter çikolatanın sağlık üzerine etkileri pek çok çalışmada değerlendirilmişancak tutarlı sonuçlar elde edilememiştir. Bazı araştırmalar, kakaonun yararlı etkisinin ancak çiğ tüketimi ile mümkünolabileceğini vurgularken, bazıları bitter çikolatanın yüksek doymuş yağ ve şeker içeriği nedeniyle vücut ağırlığında artışaneden olabileceğine dikkat çekmektedir. Bunun yanı sıra, kakao ve çikolata, teobromin ve kafein içeriğinden dolayı, belirginyüksek nabız ile ilişkilendirilmekte, bu nedenle aritmisi olanlarda ve yaşlı hastalarda tüketiminin kısıtlanması gerektiğibelirtilmektedir. Bununla birlikte, çikolatanın olumlu etkileri olduğunu gösteren bir çok çalışma da bulunmaktadır.Bu çalışmalarda, çikolatada bulunan sağlıksız bileşenlere karşılık, olumlu etkilerinin değerlendirilmesi gerektiğivurgulanmakta, istenmeyen olumsuz etkileri engellemek için uygun miktarlarda tüketilmesi gerektiği belirtilmektedir. Buderlemede, çikolata ve sağlık beyanları üzerine yapılan çalışmalar ve sonuçları değerlendirilmiştir.Article Citation - WoS: 4Citation - Scopus: 4Neuroprotective effects of adrenomedullin in experimental traumatic brain injury model in rats(Turkish Assoc Trauma Emergency Surgery, 2022) Emmez, Gokcen; Bulduk, Erkut Baha; Yildirim, ZuhalBACKGROUND: Traumatic brain injuries cause damages in the brain in several ways, which include cell death because of edema, disruption of the blood-brain barrier, shear stress, and ischemia. In this study, we investigated the effects of adrenomedullin (AM) on oxidative stress and inflammation after head traumas in a rat model. METHODS: Eighteen male adult Wistar albino rats were randomized into three groups (n=6). No traumas were applied to the control (C) group. Traumas were applied in line with Marmarau trauma model in the trauma group. The rats in the AM treatment group were treated with post-traumatic 12 mu g/kg i.p. AM in addition to the trauma group. The rats were followed for 7 days in all groups and were then sacrificed. Brain tissues and blood samples were taken. RESULTS: In the trauma group, both tissue and serum MDA, TNF-alpha, and IL-6 levels were significantly increased compared to the control group (p<0.05). In the AM-treated group, serum TNF-alpha levels were significantly decreased compared to the trauma group (p<0.05). In the trauma group, both tissue and serum GSH levels were significantly decreased compared to the control group (p<0.05). In the trauma group, serum Vitamin D3 levels were significantly decreased compared to the control group (p<0.05). In the AM-treated group, both tissue and serum GSH levels were significantly increased compared to the trauma group (p<0.05). CONCLUSION: These results indicate that AM has neuroprotective effects on traumatic brain injury in a rat model.Article Citation - Scopus: 9The Effects of Eryngium Campestre Extracts on Glutathione-S Glutathione Peroxidase and Catalase Enzyme Activities;(Turkish Pharmacists Association, 2014) Güneş,M.G.; İşgör,B.S.; İşgör,Y.G.; Shomali Moghaddam,N.; Geven,F.; Yildirim,Ö.; Moghaddam, Naznoosh ShomalıEryngium campestre L. (Apiaceae) are known in Turkish folk medicine as ‘Bogadikeni’ and wildly distributed in all parts of Turkey. Infusion of different parts of this species are well known as diuretic, appetizer and stimulant. In the present study, methanol extracts from flowers and leaves of E. campestre were evaluated for their potential medicinal value in terms of biological targets participate in antioxidant defense such as glutathione-S-transferase (GST), glutathione peroxidase (GPx) and catalase (CAT). Results obtained show that the methanol extract of flowers were found to have higher amount of phenolic and flavonoid contents than the leaves. The evaluation of extracts against biological target revealed that, the maximum GST enzymes inhibition activities was observed with methanol extract from flowers of E. campestre with IC50 value of 363 ng/mL. In this study, none of the extracts evaluated were shown any reasonable GPx and CAT inhibition. © 2014, Turkish Pharmacists Association. All rights reserved.Article Kâr Payı Dağıtımı ve Nakit Akışı Belirsizliği İlişkisi: Bıst İmalat Sektörü Firmalarında Bir Uygulama(2018) Dinçergök, Burcu; Pirgaip, BurakBu çalışmanın amacı firmaların nakit akışı belirsizliklerinin nakit kâr payı dağıtımtutarına ve olasılığına etkilerinin ortaya konulmasıdır. Bu amaç doğrultusunda Borsaİstanbul’da (BIST) işlem gören 165 adet imalat sektörü firmasının 2005-2017arasındaki yıllık verileri klasik panel veri ve panel logit veri modelleriyle analize tabitutulmuştur. İlgili modellerde diğer kontrol değişkenleri konuya ilişkin literatürdesıklıkla kullanılan serbest nakit akışları, aktif büyüklüğü, finansal kaldıraç, piyasadeğeri/defter değeri, kârlılık, dağıtılmamış kârlar/toplam özsermaye olarakbelirlenmiştir. Analiz sonuçları, firmaların nakit akışı belirsizliklerinin dağıtılan kârpayı tutarını ve olasılığını olumsuz yönde etkilediğini göstermektedir. Buna göre,firmalar nakit akışı belirsizliklerine maruz kaldıklarında nakitlerini kâr payı olarakdağıtmak yerine korumayı tercih etmektedir. Ayrıca, kâr payı dağıtımı ile serbest nakitakışı, aktif büyüklüğü, piyasa değeri/defter değeri, kârlılık, dağıtılmamış kârlar/toplamözsermaye arasında pozitif; finansal kaldıraç arasında ise, negatif ilişki bulunmuştur.Article Citation - WoS: 8Citation - Scopus: 9Diplotaxis Tenuifolia ve Reseda Lutea Metanol Özütünün Antioksidan Savunma Sistemi Enzimleri ve Aldoz Redüktaz Aktivitesi Üzerinde Olan Etkisinin İncelenmesi(Turkish Pharmacists Assoc, 2018) Abdalrahman, Khalid Sharro; Güneş, Merve Gülşah; Shomalı, Naznoosh; İşgör, Belgin Sultan; Yıldırım, ÖzlemAmaç: Bu çalışmada Diplotaxis tenuifolia ve Reseda lutea’nın çiçek ve yapraklarından elde edilmiş olan metanol özütlerinin AR, CAT, GST ve GPx enzimlerinin aktiviteleri üzerinde olan etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmada, bitki örneklerinin toplam fenolik ve flavonoid içeriği; Folin-Ciocalteu ve alüminyum klorür reaktiflerinin yardımıyla kolorimetrik yöntemlerle değerlendirilmiştir. Ayrıca özütlerin CAT, GST, GPx ve AR enzimlerinin aktiviteleri üzerindeki etkileri kinetik analizler ile araştırılmıştır. Bulgular: En yüksek miktarda fenolik ve flavanoid içeriği sırasıyla 144.49±0.29 mg gallik asit eş değeri/L ve 250.485±0.002 quercetin eş değeri/L tespit edilmiştir. GST ve GPx için en iyi aktivite profilleri sırasıyla 121±0.05 ve 140±0.001 ng/mL IC50 değerleri ile D. tenuifolia yaprak özütünde gözlemlenmiştir. Elde edilen sonuçlara göre, R. lutea ve D. tenuifolia’nın yapraklarından elde edilen metanol özütleri, AR enzimi üzerinde önemli ölçüde bir aktivite potansiyeli göstermemiştir. Bununla beraber, çalışılmış olan çiçek ve yaprak özütlerinin hiçbiri yeterli düzeyde CAT aktivasyonu gösterememiştir. Sonuç: Çalışma sonucunda, D. tenuifolia’nın yapraklarının antioksidan enzimatik savunma sistemi üzerinde iyi bir etkiye sahip olduğu gösterilmiştir. Bu sebeple günlük diyet için iyi bir besin kaynağı olarak kabul edilebilir.Article Investigation of the Anti-Inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharide-Induced Sepsis in Rats(Galenos Yayincilik, 2022) Cobaner, Nurdan; Yelken, Birgul; Erkasap, Nilufer; Ozkurt, Mete; Bektur, EzgiObjective: Corticosteroids are one of the treatment methods used to prevent inflammation in sepsis. This study aimed to determine the anti-inflammatory activity of astaxanthin in sepsis and compare it with dexamethasone. Materials and Methods: After approval of the local ethics committee, 40 Sprague-Dawley male rats were randomly assigned to the control group (n=8), lipopolysaccharide group (n=8), astaxanthin group (n=8), astaxanthin + lipopolysaccharide group (n=8) and dexamethasone + lipopolysaccharide group (n=8). On day 1, these groups were given dimethyl sulfoxide, Salmonella typhimurium lipopolysaccharide, astaxanthin dissolved in dimethyl sulfoxide, astaxanthin and lipopolysaccharide and dexamethasone and lipopolysaccharide, respectively. After 24 hours, rats underwent laparotomy, and liver and blood samples were taken. GraphPad Prism 6 was used for statistical analysis. P values less than 0.05 were considered significant. Results: Nuclear factor-kappa B levels in both treatment groups significantly decreased when compared with the lipopolysaccharide group. Apoptotic cells and reaction severity decreased significantly in the treatment groups compared with the lipopolysaccharide group. Conclusion: This study revealed that the use of astaxanthin had a positive effect on liver tissue undergoing treatment for sepsis. Moreover, despite some differences, measurement values were comparable when dexamethasone was administered.Article THE EFFECT OF PROJECT SUPERVISION TRAINING ON TEACHERS’ VIEWS OF NATURE OF SCIENTIFIC INQUIRY(2021) Özdemir, Alev; Mirici, Semra; Çiğdemoğlu, CeyhanThe study aimed to investigate the effect of project supervision training offered to Biology teachers on their views of the nature of scientific inquiry. We employed an action research design, as one of the qualitative research methods in this study. The participants were composed of 39 Biology teachers who had attended training on project supervision. The data were collected using Views of Scienetific Inquiry (VOSI) instrument developed by Schwarts, Lederman, and Lederman (2008). Following that, and a semi-structured interview form developed by the researchers was conducted. The data were analyzed using content analysis methods through creating codes and themes. The results revealed that the project supervision training supported development of teachers on five dimensions; scientific research is directed by questions, use of different methods in scientific investigations, research has many purposes, justification of scientific knowledge, and differences between scientific data and evidence. It was also found out that the training had positive effects on their professional practices.
