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Article Citation - WoS: 9Citation - Scopus: 9Dielectric Barrier Discharge and Jet Type Plasma Surface Modifications of Hybrid Polymeric Poly (ε-caprolactone)/Chitosan Scaffolds(Sage Publications Ltd, 2018) Ozkan, Ozan; Sasmazel, Hilal Turkoglu; Turkoglu Sasmazel, HilalIn this study, dry air plasma jet and dielectric barrier discharge Ar+O-2 or Ar+N-2 plasma modifications and their effects on wettability, topography, functionality and biological efficiency of the hybrid polymeric poly (epsilon-caprolactone)/chitosan scaffolds were reported. The samples treated with Ar+O-2 dielectric barrier discharge plasma (80 sccm O-2 flow rate, 3-min treatment) or with dry air plasma jet (15-cm nozzle-sample distance, 13-min treatment) had the closest wettability (49.11 +/- 1.83 and 53.60 +/- 0.95, respectively) to the commercial tissue culture polystyrene used for cell cultivation. Scanning electron microscopy images and X-ray photoelectron spectrometry analysis showed increase in topographical roughness and OH/NH2 functionality, respectively. Increased fluid uptake capacity for the scaffolds treated with Ar+O-2 dielectric barrier discharge plasma (73.60%+/- 1.78) and dry air plasma jet (72.48%+/- 0.75) were also noted. Finally, initial cell attachment as well as seven-day cell viability, growth and proliferation performances were found to be significantly better for both plasma treated scaffolds than for untreated scaffolds.Article Citation - WoS: 57Citation - Scopus: 60Atmospheric Plasma Surface Modifications of Electrospun Pcl/Chitosan Hybrid Scaffolds by Nozzle Type Plasma Jets for Usage of Cell Cultivation(Elsevier Science Bv, 2016) Surucu, Seda; Masur, Kai; Sasmazel, Hilal Turkoglu; Von Woedtke, Thomas; Weltmann, Klaus Dieter; Turkoglu Sasmazel, HilalThis paper reports Ar gas, Ar + O-2, Ar + O-2 + N-2 gas mixtures and dry air plasma modifications by atmospheric pressure argon driven kINPen and air driven Diener (PlasmaBeam) plasma jets to alter surface properties of three dimensional (3D), electrospun PCL/Chitosan/PCL layer by layer hybrid scaffolds to improve human fibroblast (MRC5) cell attachment and growth. The characterizations of the samples were done by contact angle (CA) measurements, scanning electron microscopy (SEM), X-Ray Photoelectron spectroscopy (XPS) analysis. The results showed that the plasma modification carried out under dry air and Ar + O-2 + N-2 gas mixtures were altered effectively the nanotopography and the functionality of the material surfaces. It was found that the samples treated with Ar + O-2 + N-2 gas mixtures for 1 min and dry air for 9 min have better hydrophilicity 78.9 degrees + 1.0 and 75.6 degrees + 0.1, respectively compared to the untreated samples (126.5 degrees). Biocompatibility performance of the scaffolds was determined with alamarBlue (aB) assay and MTT assay methods, Giemsa staining, fluorescence microscope, confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM) analyses. The results showed that plasma treated samples increased the hydrophilicity and oxygen functionality and topography of the surfaces significantly, thus affecting the cell viability and proliferation on/within scaffolds. (C) 2016 Elsevier B.V. All rights reserved.
