Atmospheric plasma surface modifications of electrospun PCL/chitosan/PCL hybrid scaffolds by nozzle type plasma jets for usage of cell cultivation

No Thumbnail Available

Date

2016

Authors

Journal Title

Journal ISSN

Volume Title

Publisher

Elsevier Science Bv

Research Projects

Organizational Units

Thumbnail Image
Organizational Unit
Metallurgical and Materials Engineering
(2004)
The main fields of operation for Metallurgical and Materials Engineering are production of engineering materials, defining and improving their features, as well as developing new materials to meet the expectations at every aspect of life and the users from these aspects. Founded in 2004 and graduated its 10th-semester alumni in 2018, our Department also obtained MÜDEK accreditation in the latter year. Offering the opportunity to hold an internationally valid diploma through the accreditation in question, our Department has highly qualified and experienced Academic Staff. Many of the courses offered at our Department are supported with various practice sessions, and internship studies in summer. This way, we help our students become better-equipped engineers for their future professional lives. With the Cooperative Education curriculum that entered into effect in 2019, students may volunteer to work at contracted companies for a period of six months with no extensions to their period of study.

Journal Issue

Abstract

This paper reports Ar gas, Ar + O-2, Ar + O-2 + N-2 gas mixtures and dry air plasma modifications by atmospheric pressure argon driven kINPen and air driven Diener (PlasmaBeam) plasma jets to alter surface properties of three dimensional (3D), electrospun PCL/Chitosan/PCL layer by layer hybrid scaffolds to improve human fibroblast (MRC5) cell attachment and growth. The characterizations of the samples were done by contact angle (CA) measurements, scanning electron microscopy (SEM), X-Ray Photoelectron spectroscopy (XPS) analysis. The results showed that the plasma modification carried out under dry air and Ar + O-2 + N-2 gas mixtures were altered effectively the nanotopography and the functionality of the material surfaces. It was found that the samples treated with Ar + O-2 + N-2 gas mixtures for 1 min and dry air for 9 min have better hydrophilicity 78.9 degrees + 1.0 and 75.6 degrees + 0.1, respectively compared to the untreated samples (126.5 degrees). Biocompatibility performance of the scaffolds was determined with alamarBlue (aB) assay and MTT assay methods, Giemsa staining, fluorescence microscope, confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM) analyses. The results showed that plasma treated samples increased the hydrophilicity and oxygen functionality and topography of the surfaces significantly, thus affecting the cell viability and proliferation on/within scaffolds. (C) 2016 Elsevier B.V. All rights reserved.

Description

von Woedtke, Thomas/0000-0002-1097-4832; Turkoglu Sasmazel, Hilal/0000-0002-0254-4541

Keywords

MRC5 fibroblast cells, Atmospheric plasma jet kINPen, Atmospheric plasma jet Diener (PlasmaBeam), Electrospun PCL, Electrospun chitosan, Hybrid scaffolds

Turkish CoHE Thesis Center URL

Citation

51

WoS Q

Q1

Scopus Q

Source

Volume

385

Issue

Start Page

400

End Page

409

URI

https://doi.org/10.1016/j.apsusc.2016.05.123
 https://hdl.handle.net/20.500.14411/684

Collections

WOS
Scopus