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Article Citation - WoS: 24Citation - Scopus: 28Development of Amoxicillin-Loaded Electrospun Polyurethane/Chitosan Β-Tricalcium Phosphate Scaffold for Bone Tissue Regeneration(Ieee-inst Electrical Electronics Engineers inc, 2018) Topsakal, Aysenur; Uzun, Muhammet; Ugar, Gaye; Ozcan, Aslihan; Altun, Esra; Oktar, Faik Nuzhet; Gunduz, OguzhanBiocompatible nanocomposite electrospun fibers containing Polyurethane/Chitosan/beta-Tri calcium phosphate with diverse concentrations were designed and produced through the electrospinning process for bone tissue engineering applications. After the production process, density measurement, viscosity, electrical conductivity, and tensile strength measurement tests were carried out as physical analyses of blended solutions. The chemical structural characterization was scrutinized using Fourier transform infrared spectrometer (FTIR), and scanning electron microscopy (SEM) was used to observe the morphological details of developed electrospun scaffolds. Cell viability, attachment, and proliferation were performed using a L929 fibroblast cell line. Based on the physical, SEM, FTIR analysis, and cell culture studies, preferable nanofiber composition was selected for further studies. Amoxicillin (AMX) was loaded to that selected nanofiber composition for examination of the drug release. In comparison with other studies on similar AMX controlled products, higher drug loading and encapsulation efficiencies were obtained. It has been clearly found that the developed nanofiber composites have potential for bone tissue engineering applications.Article Citation - WoS: 21Citation - Scopus: 18Dbd Atmospheric Plasma-Modified, Electrospun, Layer-By Polymeric Scaffolds for L929 Fibroblast Cell Cultivation(Taylor & Francis Ltd, 2016) Surucu, Seda; Sasmazel, Hilal TurkogluThis paper reported a study related to atmospheric pressure dielectric barrier discharge (DBD) Ar+O-2 and Ar+N-2 plasma modifications to alter surface properties of 3D PCL/Chitosan/PCL layer-by-layer hybrid scaffolds and to improve mouse fibroblast (L929 ATCC CCL-1) cell attachment, proliferation, and growth. The scaffolds were fabricated using electrospinning technique and each layer was electrospun sequentially on top of the other. The surface modifications were performed with an atmospheric pressure DBD plasma under different gas flow rates (50, 60, 70, 80, 90, and 100sccm) and for different modification times (0.5-7min), and then the chemical and topographical characterizations of the modified samples were done by contact angle (CA) measurements, scanning electron microscopy (SEM), atomic force microscopy, and X-ray photoelectron spectroscopy. The samples modified with Ar+O-2 plasma for 1min under 70cm(3)/min O-2 flow rate (71.077 degrees +/- 3.578) showed a 18.83% decrease compare to unmodified samples' CA value (84.463 degrees +/- 3.864). Comparing with unmodified samples, the average fiber diameter values for plasma-modified samples by Ar+O-2 (1min 70sccm) and Ar+N-2 (40s 70sccm) increased 40.756 and 54.295%, respectively. Additionally, the average inter-fiber pore size values exhibited decrease of 37.699 and 48.463% for the same Ar+O-2 and Ar+N-2 plasma-modified samples, respectively, compare to unmodified samples. Biocompatibility performance was determined with MTT assay, fluorescence, Giemsa, and confocal imaging as well as SEM. The results showed that Ar+O-2-based plasma modification increased the hydrophilicity and oxygen functionality of the surface, thus affecting the cell viability and proliferation on/within scaffolds.Article Citation - WoS: 6Citation - Scopus: 7Hybrid Polymeric Scaffolds Prepared by Micro and Macro Approaches(Taylor & Francis As, 2017) Ozkan, Ozan; Sasmazel, Hilal TurkogluPolymeric scaffolds with complex porous structures were fabricated with two different polymers by combining three fabrication methods in three steps, in which, nonwoven poly(e-caprolactone) microfibers were obtained with electrospinning and immersed in solvent cast chitosan solution poured in Petri dish to fabricate hybrid polymers, and finally the combined structure was freeze-dried with two different predrying techniques to obtain macropores in the structure. The resulting hybrid polymeric mats were found to have both microfibers and macroporosity due to the electrospinning as well as freeze-drying processes, which resemble the natural extracellular matrix. The optimized scaffolds that predried in the incubator at 40 degrees C for 5 h and then freeze-dried for 24 h exhibited contact angle value of 68.93 +/- 2.18 degrees with 3.252 +/- 0.783 MPa Young's modulus and 0.260 +/- 0.002 MPa yield strength as well as 1.35-fold cell yield in MRC5 fibroblast cell culture, compared to the commercial tissue culture polystyrene. [GRAPHICS] .
