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Article Citation - WoS: 45Citation - Scopus: 46Novel Hybrid Scaffolds for the Cultivation of Osteoblast Cells(Elsevier, 2011) Sasmazel, Hilal TurkogluIn this study, natural biodegradable polysaccharide, chitosan, and synthetic biodegradable polymer, poly(epsilon-caprolactone) (PCL) were used to prepare 3D, hybrid polymeric tissue scaffolds (PCL/chitosan blend and PCL/chitosan/PCL layer by layer scaffolds) by using the electrospinning technique. The hybrid scaffolds were developed through HA addition to accelerate osteoblast cell growth. Characteristic examinations of the scaffolds were performed by micrometer, SEM, contact angle measurement system, ATR-FTIR, tensile machine and swelling experiments. The thickness of all electrospun scaffolds was determined in the range of 0.010 +/- 0.001-0.012 +/- 0.002 mm. In order to optimize electrospinning processes, suitable bead-free and uniform scaffolds were selected by using SEM images. Blending of PCL with chitosan resulted in better hydrophilicity for the PCL/chitosan scaffolds. The characteristic peaks of PCL and chitosan in the blend and layer by layer nanofibers were observed. The PCL/chitosan/PCL layer by layer structure had higher elastic modulus and tensile strength values than both individual PCL and chitosan structures. The layer by layer scaffolds exhibited the PBS absorption values of 184.2; 197.2% which were higher than those of PCL scaffolds but lower than those of PCL/chitosan blend scaffolds. SaOs-2 osteosarcoma cell culture studies showed that the highest ALP activities belonged to novel PCL/chitosan/PCL layer by layer scaffolds meaning better cell differentiation on the surfaces. (C) 2011 Elsevier B.V. All rights reserved.Article Citation - WoS: 10Citation - Scopus: 7Synergistic effect of fabrication and stabilization methods on physicochemical and biological properties of chitosan scaffolds(Taylor & Francis As, 2021) Gultan, Tugce; Bektas Tercan, Seyma; Cetin Altindal, Damla; Gumusderelioglu, MenemseIn this study, the aim was to investigate the changes in the physical, chemical and biological properties of chitosan scaffolds obtained by freeze-drying and microwave-assisted gas foaming methods. Also, it was aimed to determine the most suitable one when scaffolds are subjected to different stabilization processes. To prevent the solubility of chitosan scaffolds, stabilization processes were carried out by treatment with ethanol (EtOH), sodium hydroxide (NaOH), or sodium bicarbonate (Na2CO3). The chemical and physical changes in the chitosan structure induced by different stabilization methods were investigated by characterization studies carried out comparatively with two-dimensional chitosan films. The results showed that, particularly, NaOH stabilization improved the physical, thermal, and mechanical properties of the bulk material. The wettability and surface roughness of the chitosan films were enhanced for cellular adhesion after stabilization. Cell culture studies revealed that variations in both fabrication and stabilization methods significantly affected in vitro cellular responses such as cell attachment, proliferation and viability. In conclusion, chitosan scaffolds fabricated by microwave-assisted gas foaming and stabilized by NaOH solution were found as the best structure due to their higher cellular activities.Article Citation - WoS: 6Citation - Scopus: 7Hybrid Polymeric Scaffolds Prepared by Micro and Macro Approaches(Taylor & Francis As, 2017) Ozkan, Ozan; Sasmazel, Hilal Turkoglu; Turkoglu Sasmazel, HilalPolymeric scaffolds with complex porous structures were fabricated with two different polymers by combining three fabrication methods in three steps, in which, nonwoven poly(e-caprolactone) microfibers were obtained with electrospinning and immersed in solvent cast chitosan solution poured in Petri dish to fabricate hybrid polymers, and finally the combined structure was freeze-dried with two different predrying techniques to obtain macropores in the structure. The resulting hybrid polymeric mats were found to have both microfibers and macroporosity due to the electrospinning as well as freeze-drying processes, which resemble the natural extracellular matrix. The optimized scaffolds that predried in the incubator at 40 degrees C for 5 h and then freeze-dried for 24 h exhibited contact angle value of 68.93 +/- 2.18 degrees with 3.252 +/- 0.783 MPa Young's modulus and 0.260 +/- 0.002 MPa yield strength as well as 1.35-fold cell yield in MRC5 fibroblast cell culture, compared to the commercial tissue culture polystyrene. [GRAPHICS] .
