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    Article
    Citation - WoS: 3
    Citation - Scopus: 3
    Development and Validation of a Sensitive Assay for the Quantification of Arachidonoylcyclopropylamide (acpa) in Cell Culture by Lc-ms/Ms
    (Springer int Publ Ag, 2023) Boyacioglu, Ozge; Recber, Tuba; Kir, Sedef; Korkusuz, Petek; Nemutlu, Emirhan
    Synthetic and natural cannabinoid derivatives are highly investigated as drug candidates due to their antinociceptive, antiepileptic and anticancer potential. Arachidonoylcyclopropylamide (ACPA) is a synthetic cannabinoid with antiproliferative and apoptotic effects on non-small cell lung cancer and pancreatic and endometrial carcinoma. Thus, ACPA has a great potential for being used as an anticancer drug for epithelial cancers. Therefore, determining the levels of ACPA in biological fluids, cells, tissues and pharmaceutical dosage forms is crucial in monitoring the effects of various pharmacological, physiological and pathological stimuli on biological systems. However, the challenge in the quantification of ACPA is its short half-life and lack of UV signal. Therefore, we developed a liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for sensitive and selective quantification of ACPA in cell culture medium and intracellular matrix. Multiple reaction monitoring in the positive ionization mode was used for detection with 344 -> 203 m/z transitions. The separation of ACPA was performed on C18 column (50 x 3.0 mm, 2.1 mu m) with the mobile phase run in the gradient mode with 0.1% formic acid (FA) in water and 0.1% FA in acetonitrile at a flow rate of 0.3 ml/min. The assay was linear in the concentration range of 1.8-1000 ng/mL (r = 0.999). The validation studies revealed that the method was linear, sensitive, accurate, precise, selective, repeatable, robust and rugged. Finally, the developed method was applied to quantify ACPA in cell culture medium and intracellular matrix.
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    Article
    Citation - WoS: 5
    Citation - Scopus: 9
    The mediating role of FoMO and the moderating role of narcissism in the impact of social exclusion on compulsive buying: a cross-cultural study
    (Springer int Publ Ag, 2023) Mert, Merve; Tengilimoglu, Dilaver
    Background There is an interrelationship between the concepts of social exclusion, compulsive buying behavior, fear of missing out (FoMO), and narcissism. Nevertheless, the extent to which these concepts mediate or moderate their relationships with each other has not been efficiently investigated. Objective This research aims to investigate how FoMO mediates and narcissism moderates the correlation between social exclusion and compulsive buying behavior. In addition, the research aims to test a conceptual model and highlight the differences that may occur in the conceptual model proposed in two different countries. Methods This model was analyzed among 1007 university students (Turkey = 506, Denmark = 501). The study used scales to measure social exclusion, compulsive buying behavior, FoMO, and narcissism. The study employed PROCESS Model 4 to analyze direct and indirect (mediation) effects and PROCESS Model 59 to assess conditional (moderation) effects. Furthermore, the Johnson-Neyman technique was utilized to investigate interaction terms. Results The findings indicate that those who face social exclusion tend to participate more in compulsive buying, and this connection is partly explained by FoMO. This suggests that individuals who encounter social exclusion may have an increased likelihood of experiencing FoMO, which may subsequently contribute to compulsive buying behavior. Furthermore, the moderating effect of narcissism differed between the Turkey and Danish samples. Specifically, in the Turkey sample, narcissism only modified the connection between social exclusion and FoMO, while in the Danish sample, it impacted both the connection between social exclusion and FoMO and the connection between FoMO and compulsive buying. Conclusion The obtained results show that the regulating role of narcissism is different in Turkey and Denmark within the conceptual model we studied.