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Article Citation - Scopus: 1An Investigation on the Dna Binding Activities of Melamine, Cyanuric Acid and Uric Acid(Editura Acad Romane, 2021) Senol, Ali; Devrim, Alparslan Kadir; Sudagidan, Mert; Ozalp, Veli CengizMelamine can be added to various foods such as milk, milk powder, baby food, pet, and livestock feed for cheating purposes due to its high nitrogen content. Regarding its usage in food products, there is a need to investigate its possible interactions with DNA. Thus, this study aimed to investigate the interactions of melamine and its metabolized products, cyanuric acid and uric acid with genomic DNA, isolated from eukaryotic (calf thymus) and prokaryotic (Staphylococcus aureus) sources. UV-absorbance spectrophotometry, fluorescence spectrophotometry, and agarose gel electrophoresis techniques were used to evaluate these interactions. The five different concentrations of melamine, cyanuric acid, and uric acid were incubated with fixed DNA concentration and it was determined that the test compounds interacted with the DNA molecules. The data obtained by UV-absorbance and fluorescence spectrophotometry techniques revealed an increase in wave peaks observed with the increasing substance concentration. After the obtained data of the aforementioned techniques were evaluated together, it was concluded that melamine, cyanuric acid, and uric acid bonded to the eukaryotic and prokaryotic genomic DNA materials via groove binding.Article Citation - WoS: 28Citation - Scopus: 30Microbial Community of Soda Lake Van as Obtained From Direct and Enriched Water, Sediment and Fish Samples(Nature Portfolio, 2021) Omeroglu, Esra Ersoy; Sudagidan, Mert; Yurt, Mediha Nur Zafer; Tasbasi, Behiye Busra; Acar, Elif Esma; Ozalp, Veli Cengiz; Ersoy Omeroglu, EsraSoda lakes are saline and alkaline ecosystems that are considered to have existed since the first geological records of the world. These lakes support the growth of ecologically and economically important microorganisms due to their unique geochemistry. Microbiota members of lakes are valuable models to study the link between community structure and abiotic parameters such as pH and salinity. Lake Van is the largest endroheic lake and in this study, bacterial diversity of lake water, sediment, and pearl mullet (inci kefali; Alburnus tarichi), an endemic species of fish which are collected from different points of the lake, are studied directly and investigated meticulously using a metabarcoding approach after pre-enrichment. Bacterial community structures were identified using Next Generation Sequencing of the 16S rRNA gene. The analysis revealed that the samples of Lake Van contain high level of bacterial diversity. Direct water samples were dominated by Proteobacteria, Cyanobacteria, and Bacteroidota, on the other hand, pre-enriched water samples were dominated by Proteobacteria and Firmicutes at phylum-level. In direct sediment samples Proteobacteria, whereas in pre-enriched sediment samples Firmicutes and Proteobacteria were determined at highest level. Pre-enriched fish samples were dominated by Proteobacteria and Firmicutes at phylum-level. In this study, microbiota members of Lake Van were identified by taxonomic analysis.Article Citation - WoS: 7Citation - Scopus: 7Antibiotic administration in targeted nanoparticles protects the faecal microbiota of mice(Royal Soc Chemistry, 2021) Borsa, Baris A.; Sudagidan, Mert; Aldag, Mehmet E.; Baris, Isik I.; Acar, Elif E.; Acuner, Cagatay; Ozalp, Veli C.Antibiotic therapy comes with disturbances on human microbiota, resulting in changes of bacterial communities and thus leading to well-established health problems. In this study, we demonstrated that targeted teicoplanin administration maintains the faecal microbiota composition undisturbed in a mouse model while reaching therapeutic improvements for S. aureus infection.Article Citation - WoS: 7Citation - Scopus: 7Surface Plasmon Resonance Aptasensor for Soluble Icam-1 Protein in Blood Samples(Royal Soc Chemistry, 2022) Dursun, Ali Dogan; Dogan, Soner; Kavruk, Murat; Tasbasi, B. Busra; Sudagidan, Mert; Yilmaz, M. Deniz; Tuna, Bilge G.; Deniz Yilmaz, M.; Busra Tasbasi, B.Intercellular Adhesion Molecule-1 (ICAM-1) is considered to be a cancer biomarker in the assessment of metastatic potential in patients and an early indicator of atherosclerosis. A labelless biosensor based on the surface plasmon resonance (SPR) signal from the specific affinity interaction of an aptamer and a soluble ICAM-1 protein was developed for blood samples. The developed aptasensor provided real-time information on the concentration of the ICAM-1 protein in blood when integrated to a purification step based on a magnetic pull-down separation. The SPR aptasensor was highly specific with a limit of detection of 1.4/0.2 ng ml(-1), which was achieved through aptamer-functionalized silica-coated magnetic nanoparticles.Article Citation - WoS: 14Citation - Scopus: 19Determination of Bacterial Community Structure of Turkish Kefir Beverages Via Metagenomic Approach(Elsevier Sci Ltd, 2022) Yegin, Zeynep; Yurt, Mediha Nur Zafer; Tasbasi, Behiye Busra; Acar, Elif Esma; Altunbas, Osman; Ucak, Samet; Sudagidan, MertBacterial microbiota of industrially produced kefir beverages (n:33) consumed in Turkey was studied using a culture-independent method and a metagenomic approach. DNA extraction from non pre enriched and pre-enriched kefir samples was used for 16S rRNA amplicon sequencing. Kefirs were dominated by Firmicutes, followed by Actinobacteria and Proteobacteria phyla. The most abundant genera in non pre-enriched kefir beverages were Lactococcus followed by Streptococcus, Bifidobacterium, Lactobacillus, and Leuconostoc. Pre-enriched kefirs were dominated by Streptococcus followed by Lactobacillus, Lactococcus, Bifidobacterium, and Leuconostoc at the genus level. Psychroserpens, Desulfonispora, Pediococcus, Micromonospora, Fructobacillus, Mycobacterium, Acetobacter, Pseudopedobacter, and Clostridium XI genera were found only in pre-enriched kefirs. Kefirs displayed pH differences from 4.04 to 4.49 and the acidity was 0.617e0.987. In two samples, the lowest pH values were obtained with abundance of Lactobacillus helveticus and Streptococcus salivarius. This study broadens our viewpoint and strengthens future applications of kefir beverages in industrial and medical fields. (C)& nbsp;2022 Elsevier Ltd. All rights reserved.
