Surface plasmon resonance aptasensor for soluble ICAM-1 protein in blood samples

Student admission to the Atılım University Department of Nutrition and Dietetics started in 2017. Our Department is the only academic institution to offer undergraduate-level education completely in English in the field of Nutrition and Dietetics in Ankara. The studies of our department may be classified into two main categories; education and research. The current education programs are offered taking into consideration the awareness of the responsibility in offering a degree in Nutrition and Dietetics; by competent instructors in the field, and with an inter-disciplinary approach. Our aim for the future alumni of our undergraduate program is to undertake their responsibilities in the light of their information with a professional insight, and the confidence to constantly update themselves at hospitals, polyclinics, public health centers, ministries, catering institutions, food companies, universities and such where they may be employed in positions such as health care professionals, academicians, researchers, directors or policy makers.

Abstract

Intercellular Adhesion Molecule-1 (ICAM-1) is considered to be a cancer biomarker in the assessment of metastatic potential in patients and an early indicator of atherosclerosis. A labelless biosensor based on the surface plasmon resonance (SPR) signal from the specific affinity interaction of an aptamer and a soluble ICAM-1 protein was developed for blood samples. The developed aptasensor provided real-time information on the concentration of the ICAM-1 protein in blood when integrated to a purification step based on a magnetic pull-down separation. The SPR aptasensor was highly specific with a limit of detection of 1.4/0.2 ng ml(-1), which was achieved through aptamer-functionalized silica-coated magnetic nanoparticles.

Description

Kavruk, Murat/0000-0001-5331-7253; Dursun, Ali Dogan/0000-0001-9056-0025; Tuna, Bilge Guvenc/0000-0003-1348-1336; Yilmaz, Bayram/0000-0002-2674-6535; Özalp, Veli Cengiz/0000-0002-7659-5990; Yilmaz, Mahmut/0000-0001-5793-0805