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Article Citation - WoS: 17Citation - Scopus: 18Poly(ε-Caprolactone) Composites Containing Gentamicin-Loaded Β-Tricalcium Phosphate/Gelatin Microspheres as Bone Tissue Supports(Wiley, 2013) Sezer, Umran Aydemir; Aksoy, Eda Ayse; Hasirci, Vasif; Hasirci, NesrinIn this work, novel antibacterial composites were prepared by using poly(epsilon-caprolactone) (PCL) as the main matrix material, and gentamicin-loaded microspheres composed of beta-tricalcium phosphate (beta-TCP) and gelatin. The purpose is to use this biodegradable material as a support for bone tissue. This composite system is expected to enhance bone regeneration by the presence of beta-TCP and prevent a possible infection that might occur around the defected bone region by the release of gentamicin. The effects of the ratio of the beta-TCP/gelatin microspheres on the morphological, mechanical, and degradation properties of composite films as well as in vitro antibiotic release and antibacterial activities against Escherichia coli and Staphylococcus aureus were investigated. The results showed that the composites of PCL and beta-TCP/gelatin microspheres had antibacterial activities for both bacteria. (C) 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013Article Citation - WoS: 8Citation - Scopus: 11Gentamicin Loaded Β-Tricalcium Phosphate/Gelatin Composite Microspheres as Biodegradable Bone Fillers(Wiley, 2012) Sezer, Umran Aydemir; Aksoy, Eda Ayse; Durucan, Caner; Hasirci, NesrinIn this study, novel composite bone fillers with microspherical shape, biodegradable property, and antibacterial effect were designed and prepared. Various fillers with different beta-tricalcium phosphate (beta-TCP)/gelatin (G)/glutaraldeyde (GA) compositions were loaded with a model antibiotic, gentamicin. The effect of composition and preparation conditions on the release of gentamicin was investigated in in vitro conditions. Complete release were observed in 12 h for pure beta-TCP powder, and this period was extended up to 96 h as the gelatin content increased in the microspheres. Morphological and chemical structures of the microspheres, before and after the release studies, were investigated by scanning electron microscopy and Fourier transform infrared, respectively. Antibacterial activities were examined against Escherichia coli by using disc diffusion method and promising results were obtained. It is proposed that these novel beta-TCP/G/GA microspheres can be applied locally to prevent and/or eliminate infection that might occur around a defected region of hard tissue and supports the healing process. POLYM. COMPOS., 2012. (c) 2012 Society of Plastics EngineersArticle Citation - WoS: 21Citation - Scopus: 24Poly(ε-Caprolactone) Composite Scaffolds Loaded With Gentamicin-Containing Β-Tricalcium Phosphate/Gelatin Microspheres for Bone Tissue Engineering Applications(Wiley, 2014) Sezer, Umran Aydemir; Arslantunali, Damla; Aksoy, Eda Ayse; Hasirci, Vasif; Hasirci, NesrinIn this study, novel poly(epsilon-caprolactone) (PCL) composite scaffolds were prepared for bone tissue engineering applications, where gentamicin-loaded -tricalcium phosphate (-TCP)/gelatin microspheres were added to PCL. The effects of the amount of -TCP/gelatin microspheres added to the PCL scaffold on various properties, such as the gentamicin release rate, biodegradability, morphology, mechanical strength, and pore size distribution, were investigated. A higher amount of filler caused a reduction in the mechanical properties and an increase in the pore size and led to a faster release of gentamicin. Human osteosarcoma cells (Saos-2) were seeded on the prepared composite scaffolds, and the viability of cells having alkaline phosphatase (ALP) activity was observed for all of the scaffolds after 3 weeks of incubation. Cell proliferation and differentiation enhanced the mechanical strength of the scaffolds. Promising results were obtained for the development of bone cells on the prepared biocompatible, biodegradable, and antimicrobial composite scaffolds. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40110.
