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Article Citation - WoS: 4Citation - Scopus: 4Heavy Metal Inhibition on an Alternating Activated Sludge System and Its Comparison To Conventional Methods: Case Study of Cu2+(Iwa Publishing, 2021) Buaisha, Magdi; Balku, Saziye; Ozalp-Yaman, SenizIn order to understand the behaviour of wastewater treatment plants (WWTPs) with heavy metal presence, the present study evaluates the treatment process in the presence of heavy metals (Cu2+ as a case study) and compares it with the absence of heavy metals. An activated sludge model is improved by means of incorporating other novel inhibitory kinetic and settler models for this evaluation. To achieve this goal, a simulation algorithm is developed using the MATLAB code to detect any heavy metal influence on the aerobic and anoxic growth of heterotrophic and autotrophic biomass. The code also allows for a comparison of treatment plant performance with and without Cu2+ in both conventional and alternating systems. The results reveal that the presence of heavy metals, in case of the present study for Cu2+ at 0.5 mg/L, in a biological treatment system, has an inhibitory effect on the heterotrophic bacteria but more so on the autotrophic bacteria growth and it prevents nitrification and denitrification, thus negatively effecting on the nitrogen removal in the alternating systems.Article Citation - WoS: 41Citation - Scopus: 48Platinated Copper(3-Clip Complexes as Effective Dna-Cleaving and Cytotoxic Agents(Wiley-v C H verlag Gmbh, 2008) Ozalp-Yaman, Seniz; de Hoog, Paul; Amadei, Giulio; Pitie, Marguerite; Gamez, Patrick; Dewelle, Janique; Reedijk, JanThe synthesis and biological activity of three heteronuclear platinum-copper complexes based on 3-Clip-Phen are reported. These rigid complexes have been designed to alter the intrinsic mechanism of action of both the platinum moiety and the Cu(3-Clip-Phen) unit. The platinum centers of two of these complexes are coordinated to a 3-Clip-Phen moiety, an ammine ligand and two chlorides, which are either cis or trans to each other. The third complex comprises two 3-Clip-Phen units and two chloride ligands bound in a trans fashion to the platinum ion. DNA-cleavage experiments show that the complexes are highly efficient nuclease agents. In addition, a markedly difference in their aptitude to perform direct double-strand cleavage is observed, which appears to be strongly related to the ability of the platinum unit to coordinate to DNA. Indeed, complex 6 is unable to coordinate to DNA, which is reflected by its incapability to carry out double-strand breaks. Nonetheless, this complex exhibits efficient DNA-cleavage activity, and its cytotoxicity is high for several cell lines. Complex 6 shows better antiproliferate activity than both cisplatin and Cu(3-Clip-Phen) toward most cancer cell lines. Furthermore, the cytotoxicity observed for 1 is for most cell lines close to that of cisplatin, or even better. Cu(3-Clip-Phen) induces very low cytotoxic effects, but a marked migratory activity. Complex 6 presents DNA-cleavage properties comparable to the one of Cu(3-Clip-Phen), but it does not show any migratory activity. Interestingly, both Cu(3-Clip-Phen) and 6 induces vacuolisation processes in the cell in contrast to complex 1 and cisplatin. Thus, the four complexes cisplatin tested, Cu(3-Clip-Phen), I and 6 stimulate different cellular responses.Article Citation - WoS: 168Citation - Scopus: 186Heavy Metal Removal Investigation in Conventional Activated Sludge Systems(C Ej Publishing Group, 2020) Buaisha, Magdi; Balku, Saziye; Ozalp-Yaman, SenizThe combination of industrial and domestic wastewater in municipal WWTPs (waste water treatment plants) may be economically profitable, but it increases the difficulty of treatment, and also has some detrimental effects on the biomass and causes a low-quality final effluent. The present study evaluates the treatment process both in the presence and absence of heavy metals using ASM3 (activated sludge model no.3) so as to improve the model by means of incorporating other novel inhibitory kinetic and settler models. The results reveal that the presence of heavy metal, a case study for copper and cadmium at a concentration of 0.7 mgL(-1) in a biological treatment system has a negative effect on heterotrophic bacteria concentration by 25.00 %, and 8.76 % respectively. Meanwhile, there are no important changes in COD (chemical oxygen demand), SS (total suspended solids) and TN (total nitrogen) in the final effluent in the conventional system. However, all these parameters are acceptable and consistent with EU Commission Directives. The results indicate that ASM3 can predict and provide an opportunity of the operation for an activated sludge wastewater treatment plant that receives the effluent from an industrial plant.Article Citation - WoS: 2Citation - Scopus: 2Antiproliferative Activity of Platinum(ii) and Copper(ii) Complexes Containing Novel Biquinoxaline Ligands(Oxford Univ Press, 2024) El-Beshti, Hager Sadek; Gercek, Zuhal; Kayi, Hakan; Yildizhan, Yasemin; Cetin, Yuksel; Adiguzel, Zelal; Ozalp-Yaman, SenizNowadays, cancer represents one of the major causes of death in humans worldwide, which renders the quest for new and improved antineoplastic agents to become an urgent issue in the field of biomedicine and human health. The present research focuses on the synthesis of 2,3,2MODIFIER LETTER PRIME,3MODIFIER LETTER PRIME-tetra(pyridin-2-yl)-6,6MODIFIER LETTER PRIME-biquinoxaline) and (2,3,2MODIFIER LETTER PRIME,3MODIFIER LETTER PRIME-tetra(thiophen-2-yl)-6,6MODIFIER LETTER PRIME-biquinoxaline) containing copper(II) and platinum(II) compounds as prodrug candidates. The binding interaction of these compounds with calf thymus DNA (CT-DNA) and human serum albumin were assessed with UV titration, thermal decomposition, viscometric, and fluorometric methods. The thermodynamical parameters and the temperature-dependent binding constant (KMODIFIER LETTER PRIMEb) values point out to spontaneous interactions between the complexes and CT-DNA via the van der Waals interactions and/or hydrogen bonding, except Cu(ttbq)Cl2 for which electrostatic interaction was proposed. The antitumor activity of the complexes against several human glioblastomata, lung, breast, cervix, and prostate cell lines were investigated by examining cell viability, oxidative stress, apoptosis-terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, in vitro migration and invasion, in vitro-comet DNA damage, and plasmid DNA interaction assays. The U87 and HeLa cells were investigated as the cancer cells most sensitive to our complexes. The exerted cytotoxic effect of complexes was attributed to the formation of the reactive oxygen species in vitro. It is clearly demonstrated that Cu(ttbq)Cl2, Pt(ttbq)Cl2, and Pt(tpbq)Cl2 have the highest DNA degradation potential and anticancer effect among the tested complexes by leading apoptosis. The wound healing and invasion analysis results also supported the higher anticancer activity of these two compounds. Graphical Abstract Antitumor activity of biqunoxaline complexes.
