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Article Citation - WoS: 124Citation - Scopus: 155Development of satureja Cuneifolia-loaded< Sodium Alginate/Polyethylene Glycol Scaffolds Produced by 3d-Printing Technology as a Diabetic Wound Dressing Material(Elsevier, 2020) Ilhan, Elif; Cesur, Sumeyye; Guler, Ece; Topal, Fadime; Albayrak, Deniz; Guncu, Mehmet Mucahit; Gunduz, OguzhanAcute wounds are a common health problem, with millions of people affected and decreased granulation tissue formation and vascularization, it is also a big challenge for wound care researchers to promote acute wound healing around the globe. This study aims to produce and characterize Satureja cuneifolia plant extract (SC) blended with sodium alginate (SA) /polyethylene glycol (PEG) scaffolds for the potential treatment of diabetic ulcer. SA/PEG scaffolds were prepared by adding different concentrations (1, 3, and 5 wt%) of PEG to 9 wt% SA. The morphological and chemical composition of the resulting 3D printed composite scaffolds was determined using scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR), respectively. Mechanical and thermal properties, swelling, and degradation behaviours were also investigated. The release kinetics of SC were performed. The antimicrobial analysis was evaluated against Escherichia coli and Staphylococcus aureus strains. 3D printed scaffolds have shown an excellent antibacterial effect, especially against gram-positive bacteria due to the antibacterial SC extract they contain. Furthermore, the cell viability of fibroblast (L929) cells on/within scaffolds were determined by the colourimetric MTT assay. The SA/PEG/SC scaffolds show a great promising potential candidate for diabetic wound healing and against bacterial infections. (c) 2020 Elsevier B.V. All rights reserved.Article Citation - WoS: 7Citation - Scopus: 7Advanced 3d Printed Bone Scaffolds With Sodium Alginate/Tri-calcium Phosphate/Probiotic Bacterial Hydroxyapatite: Enhanced Mechanical and Biocompatible Properties for Bone Tissue Engineering(Elsevier Sci Ltd, 2024) Nouri, Sabereh; Emtiazi, Giti; Ulag, Songul; Gunduz, Oguzhan; Koyuncu, Ayse Ceren Calikoglu; Roghanian, Rasoul; Sasmazel, Hilal TurkogluIntroduction: The increasing prevalence of severe bone diseases, such as osteoporosis and critical bone defects, necessitates the development of more effective bone substitutes. This study addresses this need by investigating 3D-printed bone scaffolds composed of sodium alginate and tricalcium phosphate, enhanced with three distinct types of hydroxyapatite (HA): bovine-derived HA, commercially available HA, and HA enriched with probiotic bacteria. We aim to evaluate the performance of these scaffolds in terms of mechanical strength, biocompatibility, and their ability to support bone regeneration. Methods: The scaffolds were analyzed through various tests, including X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) to characterization. Scanning Electron Microscopy (SEM) was used to examine pore structure, while swelling and degradation tests evaluated the scaffold's stability. Compression testing determined mechanical strength, and in vitro cell culture assays assessed cell proliferation, osteogenic differentiation, and biomineralization. Results: SEM results indicated that 3D scaffolds with probiotic bacterial HA had the desired 472 mu m pore size. These scaffolds demonstrated a strain of 29.26 % and a compressive strength of 10 MPa, meeting the mechanical standards of human trabecular bone. Cell culture studies revealed enhanced cell proliferation by 50 %, osteogenic differentiation with 15.3 U/mg ALP activity, and 1.22-fold biomineralization, suggesting they are highly biocompatible and promote bone growth. Conclusion: Probiotic bacterial HA scaffolds exhibit ideal properties and biocompatibility, enhancing bone regeneration and serving as an ideal alternative to chemical types.
