Filters

2023 - 20262
Reset filters

Settings

Publication Index: WoSSubject: Enterococci

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Article
    Novel Enterococcus Phages Identified through Comprehensive Screening to Control Contamination in Chicken Meat
    (Wiley, 2026) Unal, Gultekin; Cengiz, Gorkem; Cufaoglu, Gizem; Acar, Bahar Onaran; Ayaz, Naim Deniz; Yildiz, Tansu; Erdinc, Ayse Nur
    BACKGROUND Antibiotic-resistant Enterococcus faecalis and E. faecium are persistent contaminants in food and environmental settings, including poultry-related matrices, contributing to food safety risks and antimicrobial resistance (AMR) dissemination. This study aimed to identify Enterococcus phages through environmental screening and evaluate their biocontrol potential using a chicken wing food model. RESULTS From 1719 environmental samples, 45 Enterococcus-targeting phages were isolated, and two (Efs.1 1-1 and Efm 3-10) with the broadest lytic profiles were selected for characterization. Transmission electron microscopy revealed that both phages belong to Caudoviricetes with siphovirus-like morphology, latent periods (6-8 min), and high burst sizes (90-110 PFU per cell). Whole-genome sequencing revealed complete circular genomes with estimated completeness values between 0.97 and 1.00, and no detectable virulence, lysogeny, or AMR genes. The phages were stable at pH 4 for 60 min and tolerated 40-60 degrees C for 1 h. Phage titers decreased from 10.0 to no less than 6.0 log PFU mL(-1) across all storage conditions over 12 months. In vitro assays in tryptic soy broth showed that while the control groups reached 8.56-9.12 log CFU mL(-1) at 24 h at 37 degrees C, no bacteria were detected in any of the phage-treated samples (limit of detection: 1 CFU mL(-1)). In the chicken wing food model, phage treatment maintained bacterial counts below the detection limit (<1 log CFU g(-1)) throughout refrigerated storage. Mean log reductions reached 1.78-2.78 and 3.71-4.71 log CFU g(-1), respectively. CONCLUSION These novel lytic phages exhibit strong stability and rapid antibacterial activity, offering promising biocontrol agents to reduce Enterococcus contamination in chicken meat.
  • Thumbnail Image
    Article
    Citation - WoS: 5
    Citation - Scopus: 6
    Bacterial and Fungal Microbiota of Mould-Ripened Cheese Produced in Konya
    (Wiley, 2023) Yurt, Mediha Nur Zafer; Omeroglu, Esra Ersoy; Tasbasi, Behiye Busra; Acar, Elif Esma; Altunbas, Osman; Ozalp, Veli Cengiz; Sudagidan, Mert; Ersoy Omeroglu, Esra
    Bacterial and fungal diversities of 24 mould-ripened cheeses originating from Konya-Turkiye were examined by metagenomic analysis. Firmicutes phylum, Enterococcus, Clostridium sensu stricto and Lactobacillus (Levilactobacillus) genera were the dominant bacteria. Ascomycota phylum and Penicillium and Pichia genera and Penicillium roqueforti and Pichia membranifaciens species were dominant fungi. Enterococcus faecium (n = 30) and Enterococcus faecalis (n = 6) were identified, and all strains were susceptible to penicillin, ampicillin, vancomycin, teicoplanin, chloramphenicol and linezolid. The highest resistance (n = 14) was against rifampin. Tetracycline resistance was determined in two strains. Biofilm-forming ability was found in nine E. faecium and 1 E. faecalis. E. faecium strains revealed 40-88.9%, and E. faecalis showed 59.2-100% homology by pulsed field gel electrophoresis.