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    Citation - WoS: 15
    Citation - Scopus: 15
    The Effect of Sevoflurane and Fullerenol C 60 on the Liver and Kidney in Lower Extremity Ischemia-Reperfusion Injury in Mice With Streptozocin-Induced Diabetes
    (Dove Medical Press Ltd, 2023) Sengel, Necmiye; Kucuk, Ayseguel; Ozdemir, Cagri; Sezen, Saban Cem; Kip, Gulay; Er, Fatma; Arslan, Mustafa
    Objective: This study aimed to demonstrate whether fullerenol C60, sevoflurane anesthesia, or a combination of both had protective effects on the liver and kidneys in lower extremity ischemia-reperfusion injury (IRI) in mice with streptozocin-induced diabetes. Methods: A total of 46 Swiss albino mice were divided into six groups as follows: control group (group C, n=7), diabetes group (group D, n=7), diabetes-ischemia/reperfusion (group DIR, n=8), diabetes-ischemia/reperfusion-fullerenol C60 (group DIR-FC60, n=8), diabetes-ischemia/reperfusion-sevoflurane (group DIR-S, n=8), and the diabetes-ischemia/reperfusion-fullerenol C60-sevoflurane (group DIR-S-FC60, n=8). Fullerenol C60 (100mg/kg) was administered intraperitoneally 30 min before the ischemia-reperfusion procedure to the fullerenol groups (DIR-FC60 and DIR-S-FC60). In the DIR groups, 2 hours (h) ischemia-2h reperfusion periods were performed. In the sevoflurane groups, sevoflurane was applied during the ischemia-reperfusion period with 100% O2. Liver and kidney tissues were removed at the end of the reperfusion procedure for biochemical and histopathological examinations.Results: In liver tissue, hydropic degeneration, sinusoidal dilatation, pycnotic nuclei, prenecrotic cells, and mononuclear cell infiltration in parenchyma were significantly more frequent in group DIR than in groups D and group C. In terms of the histopathologic criteria examined, more positive results were seen in group DIR-FC60, and when group DIR-FC60 was compared with group DIR, the difference was significant. The best results in AST, ALT, glucose, TBARS levels, and SOD enzyme activities in liver tissue were in group DIR-FC60 compared with group DIR, followed by groups DIR-S-FC60 and DIR-S, respectively. Regarding TBARS levels and SOD enzyme activities in kidney tissue, the best results were in groups DIR-FC60, DIR-S-FC60, and DIR-S, respectively.Conclusion: According to our findings, it is clear that fullerenol C60 administered intraperitoneally 30 min before ischemia, alone or together with sevoflurane, reduces oxidative stress in distant organ damage caused by lower extremity IRI, and reduces liver and kidney tissue damage in histopathologic examinations.
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    Article
    Investigation of the Anti-Inflammatory Effects of Astaxanthin on Liver Tissue in Lipopolysaccharide-Induced Sepsis in Rats
    (Galenos Yayincilik, 2022) Cobaner, Nurdan; Yelken, Birgul; Erkasap, Nilufer; Ozkurt, Mete; Bektur, Ezgi
    Objective: Corticosteroids are one of the treatment methods used to prevent inflammation in sepsis. This study aimed to determine the anti-inflammatory activity of astaxanthin in sepsis and compare it with dexamethasone. Materials and Methods: After approval of the local ethics committee, 40 Sprague-Dawley male rats were randomly assigned to the control group (n=8), lipopolysaccharide group (n=8), astaxanthin group (n=8), astaxanthin + lipopolysaccharide group (n=8) and dexamethasone + lipopolysaccharide group (n=8). On day 1, these groups were given dimethyl sulfoxide, Salmonella typhimurium lipopolysaccharide, astaxanthin dissolved in dimethyl sulfoxide, astaxanthin and lipopolysaccharide and dexamethasone and lipopolysaccharide, respectively. After 24 hours, rats underwent laparotomy, and liver and blood samples were taken. GraphPad Prism 6 was used for statistical analysis. P values less than 0.05 were considered significant. Results: Nuclear factor-kappa B levels in both treatment groups significantly decreased when compared with the lipopolysaccharide group. Apoptotic cells and reaction severity decreased significantly in the treatment groups compared with the lipopolysaccharide group. Conclusion: This study revealed that the use of astaxanthin had a positive effect on liver tissue undergoing treatment for sepsis. Moreover, despite some differences, measurement values were comparable when dexamethasone was administered.