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Article Citation - WoS: 15Citation - Scopus: 15The Effect of Sevoflurane and Fullerenol C 60 on the Liver and Kidney in Lower Extremity Ischemia-Reperfusion Injury in Mice With Streptozocin-Induced Diabetes(Dove Medical Press Ltd, 2023) Sengel, Necmiye; Kucuk, Ayseguel; Ozdemir, Cagri; Sezen, Saban Cem; Kip, Gulay; Er, Fatma; Arslan, MustafaObjective: This study aimed to demonstrate whether fullerenol C60, sevoflurane anesthesia, or a combination of both had protective effects on the liver and kidneys in lower extremity ischemia-reperfusion injury (IRI) in mice with streptozocin-induced diabetes. Methods: A total of 46 Swiss albino mice were divided into six groups as follows: control group (group C, n=7), diabetes group (group D, n=7), diabetes-ischemia/reperfusion (group DIR, n=8), diabetes-ischemia/reperfusion-fullerenol C60 (group DIR-FC60, n=8), diabetes-ischemia/reperfusion-sevoflurane (group DIR-S, n=8), and the diabetes-ischemia/reperfusion-fullerenol C60-sevoflurane (group DIR-S-FC60, n=8). Fullerenol C60 (100mg/kg) was administered intraperitoneally 30 min before the ischemia-reperfusion procedure to the fullerenol groups (DIR-FC60 and DIR-S-FC60). In the DIR groups, 2 hours (h) ischemia-2h reperfusion periods were performed. In the sevoflurane groups, sevoflurane was applied during the ischemia-reperfusion period with 100% O2. Liver and kidney tissues were removed at the end of the reperfusion procedure for biochemical and histopathological examinations.Results: In liver tissue, hydropic degeneration, sinusoidal dilatation, pycnotic nuclei, prenecrotic cells, and mononuclear cell infiltration in parenchyma were significantly more frequent in group DIR than in groups D and group C. In terms of the histopathologic criteria examined, more positive results were seen in group DIR-FC60, and when group DIR-FC60 was compared with group DIR, the difference was significant. The best results in AST, ALT, glucose, TBARS levels, and SOD enzyme activities in liver tissue were in group DIR-FC60 compared with group DIR, followed by groups DIR-S-FC60 and DIR-S, respectively. Regarding TBARS levels and SOD enzyme activities in kidney tissue, the best results were in groups DIR-FC60, DIR-S-FC60, and DIR-S, respectively.Conclusion: According to our findings, it is clear that fullerenol C60 administered intraperitoneally 30 min before ischemia, alone or together with sevoflurane, reduces oxidative stress in distant organ damage caused by lower extremity IRI, and reduces liver and kidney tissue damage in histopathologic examinations.Article The Effect of Exercise Intensity on Ischemia/Reperfusion Injury and Myokine Profile in Diabetic Cardiomyopathy(Brill, 2024) Akat, F.; Tatar, Y.; Celik, H.; Ficicilar, H.; Dursun, A. D.; Bastug, M.Diabetes is a metabolic disorder characterised by hyperglycaemia. The diabetic heart becomes more susceptible to ischemic injury. Although exercise induces a cardioprotective phenotype, the determination of accurate protocol is crucial. We compared two different exercise intensities in the diabetes model and evaluated the role of myokines in exercise-induced cardioprotection. Male, adult, Wistar albino rats were used (n = 20 each). First, animals were divided into two groups: Non-Diabetic (ND), Diabetic (DM); then groups were further divided into subgroups: Sedentary (S), Training-1 (T1 =10 m/min, 00 inclination), and Training-2 (T2 = 20 m/min, 100 inclination). Diabetes was induced by streptozotocin (60 mg/kg; i.p.). Animals exercised on a treadmill 5 days/a week for 6 weeks. Then, hearts were attached to the Langendorff apparatus and baseline functional parameters were measured. After 30'/120'I/R protocol, infarct size was evaluated with tetrazolium chloride staining. Interleukin-6, FNDC5, and myonectin levels were measured both in the soleus and the left ventricle. We observed cardiac hypertrophy and impaired baseline LV function in diabetes. Infarct size was significantly larger in diabetics and only T1 decreased the infarct size whereas T2 further aggravated it. Moreover, post-ischemic recovery was worst in diabetic-T2 group. Irisin and myonectin levels were decreased in the soleus muscle of diabetic animals. T1 increased the myonectin levels in the left ventricle of non-diabetics, and this effect was blunted in diabetic-T1 animals. As a conclusion, light-intensity exercise is a better approach to prevent ischemic damage in diabetes besides moderate intensity may be hazardous in diabetic population.
