2 results
Search Results
Now showing 1 - 2 of 2
Article Citation - WoS: 1Citation - Scopus: 1Dietary Total Antioxidant Capacity and Oxidative Stress in Patients With Type-2 Diabetes(Mattioli 1885, 2021) Cetiner, Ozlem; Sendur, Suleyman Nahit; Yalcin, Tuba; Bayraktar, Miyase; Rakicioglu, NeslisahBackground: Reactive oxygen species can disrupt normal cellular functions by damaging DNA, protein, and lipid structures of the cell. Some antioxidant molecules may protect the body against reactive oxygen species. We aimed to investigate the relationship between the dietary intake of antioxidants and oxidative DNA damage in diabetic patients. Material and Methods: A total of 85 individuals were included in the study, of which 30 were newly diagnosed with type-2 diabetes, 30 were formerly diagnosed with type-2 diabetes, and 25 were healthy individuals. Twenty-four-hour dietary recalls were recorded for 3 consecutive days. Dietary total antioxidant capacity and dietary oxidative balance scores were calculated according to these records. Spot urine samples were collected and analyzed for 8-hydroxy-2' deoxyguanosine/creatinine. Results: Dietary total antioxidant capacity, estimated via different methods, was higher in the controls than that in patients with type-2 diabetes (p<0.05). The urinary 8-hydroxy-2'-deoxyguanosine/creatinine ratio, a reliable predictor of oxidative DNA damage, was also higher in non-diabetic patients (p<0.05). The urinary 8-hydroxy-2'-deoxyguanosine/creatinine ratio was not related to dietary antioxidant intake (p>0.05). Conclusion: Urinary 8-hydroxy-2'-deoxyguanosine/creatinine concentration may not always reflect the current oxidative status of the body.Article Citation - WoS: 4Citation - Scopus: 5Antioxidant Activity of Micractinium Sp. (Chlorophyta) Extracts Against H2O2 Induced Oxidative Stress in Human Breast Adenocarcinoma Cells(Nature Portfolio, 2024) Bulut, Onur; Kose, Iskin Engin; Sonmez, Cagla; Oktem, Huseyin AvniIn response to the growing demand for high-value bioactive compounds, microalgae cultivation has gained a significant acceleration in recent years. Among these compounds, antioxidants have emerged as essential constituents in the food, pharmaceutical, and cosmetics industries. This study focuses on Micractinium sp. ME05, a green microalgal strain previously isolated from hot springs flora in our laboratory. Micractinium sp. cells were extracted using six different solvents, and their antioxidant capacity, as well as total phenolic, flavonoid, and carotenoid contents were evaluated. The methanolic extracts demonstrated the highest antioxidant capacity, measuring 7.72 and 93.80 mu mol trolox equivalents g-1 dry weight (DW) according to the DPPH and FRAP assays, respectively. To further characterize the biochemical profile, reverse phase high-performance chromatography (RP-HPLC) was employed to quantify twelve different phenolics, including rutin, gallic acid, benzoic acid, cinnamic acid, and beta-carotene, in the microalgal extracts. Notably, the acetone extracts of Micractinium sp. grown mixotrophically contained a high amount of gallic acid (469.21 +/- 159.74 mu g g-1 DW), while 4-hydroxy benzoic acid (403.93 +/- 20.98 mu g g-1 DW) was the main phenolic compound in the methanolic extracts under heterotrophic cultivation. Moreover, extracts from Micractinium sp. exhibited remarkable cytoprotective activity by effectively inhibiting hydrogen peroxide-induced oxidative stress and cell death in human breast adenocarcinoma (MCF-7) cells. In conclusion, with its diverse biochemical composition and adaptability to different growth regimens, Micractinium sp. emerges as a robust candidate for mass cultivation in nutraceutical and food applications.
