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Article Bacterial Profiling of Brined Grapevine Leaves Collected From Different Local Markets in Türkiye(John Wiley and Sons Inc, 2025) Ucak, S.; Yegin, Z.; Yurt, M.N.Z.; Sudagidan, M.; Altunbas, O.; Ozalp, V.C.Microorganisms linked to the grapevine, Vitis vinifera, can impact crop quality, plant growth, and human and plant health. This study examined the bacterial community structures of brined grapevine leaves (n = 56) taken from seven distinct regions in Türkiye using next-generation sequencing technology. Investigations were also conducted into the samples' chemical properties. Firmicutes was the dominant phylum, followed by Proteobacteria, Actinobacteria, and Bacteroidetes. Bacillaceae was the predominant family in the analyzed samples, followed by Clostridiaceae, Peptostreptococcaceae, and Enterobacteriaceae. Bacillus was the dominant genus, followed by Lysinibacillus and Clostridium sensu stricto. The samples exhibited differences in terms of pH, total acidity, and salt content. The pH values of the brined grapevine leaves ranged from 2.31 to 6.91, the acidity levels ranged from 0.09% to 1.80%, and the salt percentages ranged from 3.39% to 49.14%. This research provides pioneering information for bacterial community analysis of brined grapevine leaves. © 2025 Wiley-VHCA AG, Zurich, Switzerland.Article The Effect of a Single Session Rubber Hand Illusion on Pressure Pain Is Not Long-Lasting(John Wiley and Sons Inc, 2025) Ceylan, H.; Acet, N.; Günendi, Z.Background: Rubber hand illusion (RHI) is an experience that causes changes in body perception and awareness as a result of the integration of simultaneous perceived visual and tactile stimuli. After synchronous brush strokes with rubber and real hands, the person perceives the rubber hand as their own. RHI is known to alter pain perception. In this study, it was aimed to evaluate the effects of RHI on pressure pain threshold and continuity of this effect. Methods: Twenty-three volunteers who developed RHI were included in our study and two conditions, illusion (synchronous) and control (asynchronous), were applied. The illusion condition was created by synchronous brush strokes, while the control condition was created by asynchronous brush application using different frequency and different finger areas in the same individuals. In both conditions, pressure pain threshold measurements with an algometer were performed at four times: baseline/1st measurement, during the brush stroke/2nd measurement, at the end of the brush stroke/3rd measurement and after the hand was removed from the environment/4th measurement. Results: It was shown that RHI increased the pressure-pain threshold (p = 0.004) in healthy volunteers. Asynchronous brush strokes arranged as a control trial significantly decreased the pressure pain threshold (p = 0.002). Conclusions: It was found that the threshold values that change during the brush strokes return to the initial state after the brush strokes are terminated and the rubber hand is removed from the environment so that the effect of the illusion does not last for a long time with a single session application. © 2025 European Pain Federation - EFIC ®.Article Citation - Scopus: 1Development of a Questionnaire To Assess Phosphate Knowledge in Children With Chronic Kidney Disease and Their Caregivers(John Wiley and Sons Inc, 2025) McAlister, L.; Shaw, V.; Pugh, P.; Joyce, T.; Snauwaert, E.; Bathgate, F.; Lambert, K.Introduction: Hyperphosphataemia is a common complication of paediatric chronic kidney disease (CKD), despite the use of phosphate binders and the numerous strategies employed to reduce dietary phosphate (P) intake. This article describes the development of two self-administered semi-structured Phosphate Understanding and Knowledge Assessment (PUKA) questionnaires. The purpose of these is to assess challenges with adherence and measure declarative nutrition and procedural knowledge of phosphate in children and young people (CYP) with CKD and their caregivers. The aim is to create questionnaires that will be used for future studies investigating the relationship between knowledge and blood P-levels. Methods: Questions were generated from a literature review, clinical experience and feedback from a survey sent to UK paediatric kidney dietitians. The content, format and style of the questions were adapted and validated via expert consensus (including a psychologist, play therapist, paediatric kidney dietitians and nephrologists from the international Paediatric Renal Nutrition Taskforce, and our Young Persons’ Advisory Group), two caregivers and two CYP. A draft questionnaire was piloted with five caregivers and CYP with CKD to ensure face and content validity. To allow utilisation in a planned multi-centre trial, it was translated into five languages (Dutch, French, German, Italian and Turkish). The final English version questionnaires were used in a sample of CYP with CKD stages 4–5 and on dialysis (CKD4-5D), and caregivers, from three UK paediatric kidney centres. Results: From an initial pool of 80 questions, 37 were included in the final PUKA questionnaire. Thirteen were knowledge-based, and a knowledge score was developed. An adult and a child-friendly format were designed. Pilot testing confirmed face validity to ensure the questions were understandable. Forty-four CYP with CKD4-5D and 33 caregivers completed the final English PUKA questionnaires, with over 80% rating it easy to complete. The median time required to complete it was 11:06 min (IQR: 7:22–16:31). Conclusions: The PUKA questionnaires are a valid and reliable tool for measuring P-related knowledge and experiences of managing phosphate in CYP with CKD and their caregivers. © 2025 The British Dietetic Association Ltd.Article Citation - WoS: 1Citation - Scopus: 1Effect of Atomic Charges on C2H2/Co2 Separation Performances of Covalent-Organic Framework Adsorbents(John Wiley and Sons Inc, 2025) Demir, H.; Erucar, I.A critical factor for the accuracy of computational screening studies is the method employed to assign atomic charges. While chemically meaningful atomic charges can be obtained using a quantum chemistry method-based charge assignment technique (density-derived electrostatic and chemical method (DDEC6)), its application to large material datasets remains computationally demanding. As an alternative, machine-learning (ML) models can offer the ability to determine atomic charges with high accuracy and speed. Herein, two ML models, Partial Atomic Charge Predicter for Porous Materials based on Graph Convolutional Neural Network (PACMAN) and Partial Atomic Charges in Metal-Organic Frameworks (PACMOF), are utilized to predict atomic charges in Clean, Uniform, Refined with Automatic Tracking from Experimental Database (CURATED) covalent-organic frameworks (COFs). The predicted atomic charges are used in simulations to assess COFs' C2H2/CO2/CH4 separation performances in comparison with reference DDEC6-based performances. Results show PACMAN charges can more effectively reproduce DDEC6-based charges and corresponding separation performance metrics, underscoring their suitability for high-throughput material screening. Additionally, the proportions of Coulombic interactions to van der Waals interactions are systematically analyzed, revealing substantial variation across both narrow and wide pores. This study highlights that ML models can be applied to obtain atomic charges that could enable attaining accurate material performance evaluations. © 2025 The Author(s). Advanced Theory and Simulations published by Wiley-VCH GmbH.Article Unveiling the Impact of Vernalisation on Seed Oil Content and Fatty Acid Composition in Rapeseed (Brassica Napus L.) Through Simulated Shorter Winters(John Wiley and Sons Inc, 2025) Çağlı, İ.; Kıvrak, B.E.; Altunbaş, O.; Sönmez, Ç.Climate change is leading to warmer winters world-wide with an increasing number of extreme events every year. Studies show that winter varieties of rapeseed are particularly impacted negatively by global warming. This study investigates the molecular, physiological, and biochemical effects of diverse vernalisation scenarios (i.e., the vernalisation models) on rapeseed plants and seeds. The winter and spring varieties of rapeseed (Brassica napus L.) were subjected to short durations of vernalisation (3 and 4 weeks) as well as to 6- and 8-week long vernalisation interrupted by 1-week devernalisation intervals at warm temperatures. Our results reveal a notable difference in vernalisation responsiveness in major floral regulator FLC orthologues between the late-flowering winter variety, Darmor, the early-flowering winter variety, Bristol, and the spring variety, Helios, after 3 weeks of vernalisation. Within the three FLC genes (BnaFLCA02, BnaFLCA10, and BnaFLCC02) analysed in this study, BnaFLCA10 emerged as the most responsive to vernalisation in all three varieties. The vernalisation duration significantly influenced seed oil content and fatty acid composition in both Bristol and Helios varieties. In Bristol, the 2 + 6w vernalisation model in which vernalisation was interrupted for 1 week after 2 weeks of vernalisation and continued for another 4 weeks consistently resulted in the highest oil content and oleic acid percentage. The interrupted vernalisation (2 + 4w and 2 + 6w) also led to increased monounsaturated fatty acids across all 3 years. In Helios, non-vernalised plants produced seeds with the lowest oil content, and vernalisation duration positively correlated with both seed oil content and oleic acid percentage. Our findings unveil a robust correlation between vernalisation and seed oil content, as well as fatty acid composition in rapeseed. © 2025 The Author(s). Journal of Agronomy and Crop Science published by Wiley-VCH GmbH.
