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Article Citation - WoS: 35Citation - Scopus: 45Study on the Cytocompatibility, Mechanical and Antimicrobial Properties of 3d Printed Composite Scaffolds Based on Pva/ Gold Nanoparticles (aunp)/ Ampicillin (amp) for Bone Tissue Engineering(Elsevier, 2021) Topsakal, Aysenur; Midha, Swati; Yuca, Esra; Tukay, Ari; Sasmazel, Hilal Turkoglu; Kalaskar, Deepak M.; Gunduz, OguzhanOver the years, gold nanoparticles (AuNP) have been widely used in several biomedical applications related to the diagnosis, drug delivery, bio-imaging, photo-thermal therapy and regenerative medicine, owing to their unique features such as surface plasmon resonance, fluorescence and easy surface functionality. Recent studies showed that gold nanoparticles display positive effect on osteogenic differentiation. In line with this effect, 3-Dimesional (3D) scaffolds that can be used in bone tissue were produced by exploiting the properties of gold nanoparticles that increase biocompatibility and support bone tissue development. In addition, ampicillin was added to the scaffolds containing gold nanoparticles as a model drug to improve its antimicrobial properties. The scaffolds were produced as composites of polyvinyl alcohol (PVA) main matrix as PVA, PVA/AuNP, PVA/Ampicillin (AMP) and PVA/AuNP/AMP. Scanning Electron Microscopy (SEM) Fourier Transform Infrared Spectroscopy (FTIR), tensile measurement tests, and in vitro applications of 3D scaffolds were performed. As depicted by SEM, scaffolds were produced at pore sizes appropriate for bone tissue regeneration. According to FTIR results, there was no modification observed in the AMP, PVA and gold nanoparticles due to mixing in the resultant scaffolds. In vitro results show that 3D printed composite scaffold based on PVA/AuNP/AMP are biocompatible, osteo-inductive and exhibit antimicrobial properties, compared to PVA scaffolds. This study has implications for addressing infections during orthopedic surgeries. The PVA-based gold nanoparticle 3D tissue scaffold study containing ampicillin covers a new study compared to other articles based on gold nanoparticles.Article Citation - WoS: 39Citation - Scopus: 44A Novel Treatment Strategy for Preterm Birth: Intra-Vaginal Progesterone-Loaded Fibrous Patches(Elsevier, 2020) Cam, Muhammet Emin; Hazar-Yavuz, Ayse Nur; Cesur, Sumeyye; Ozkan, Ozan; Alenezi, Hussain; Sasmazel, Hilal Turkoglu; Edirisinghe, MohanProgesterone-loaded poly(lactic) acid fibrous polymeric patches were produced using electrospinning and pressurized gyration for infra-vaginal application to prevent preterm birth. The patches were intravaginally inserted into rats in the final week of their pregnancy, equivalent to the third trimester of human pregnancy. Maintenance tocolysis with progesterone-loaded patches was elucidated by recording the contractile response of uterine smooth muscle to noradrenaline in pregnant rats. Both progesterone-loaded patches indicated similar results from release and thermal studies, however, patches obtained by electrospinning had smaller average diameters and more uniform dispersion compared to pressurized gyration. Patches obtained by pressurized gyration had better results in production yield and tensile strength than electrospinning; thereby pressurized gyration is better suited for scaled-up production. The patches did not affect cell attachment, viability, and proliferation on Vero cells negatively. Consequently, progesterone-loaded patches are a novel and successful treatment strategy for preventing preterm birth.Article Citation - WoS: 58Citation - Scopus: 77Electrospun Oxygen Scavenging Films of Poly(3-Hydroxybutyrate) Containing Palladium Nanoparticles for Active Packaging Applications(Mdpi, 2018) Cherpinski, Adriane; Gozutok, Melike; Sasmazel, Hilal Turkoglu; Torres-Giner, Sergio; Lagaron, Jose M.This paper reports on the development and characterization of oxygen scavenging films made of poly(3-hydroxybutyrate) (PHB) containing palladium nanoparticles (PdNPs) prepared by electrospinning followed by annealing treatment at 160 degrees C. The PdNPs were modified with the intention to optimize their dispersion and distribution in PHB by means of two different surfactants permitted for food contact applications, i.e., hexadecyltrimethylammonium bromide (CTAB) and tetraethyl orthosilicate (TEOS). Analysis of the morphology and characterization of the chemical, thermal, mechanical, and water and limonene vapor barrier properties and the oxygen scavenging capacity of the various PHB materials were carried out. From the results, it was seen that a better dispersion and distribution was obtained using CTAB as the dispersing aid. As a result, the PHB/PdNP nanocomposites containing CTAB provided also the best oxygen scavenging performance. These films offer a significant potential as new active coating or interlayer systems for application in the design of novel active food packaging structures.Review Citation - WoS: 61Citation - Scopus: 72Atmospheric Pressure Plasma Surface Treatment of Polymers and Influence on Cell Cultivation(Mdpi, 2021) Sasmazel, Hilal Turkoglu; Alazzawi, Marwa; Alsahib, Nabeel Kadim AbidAtmospheric plasma treatment is an effective and economical surface treatment technique. The main advantage of this technique is that the bulk properties of the material remain unchanged while the surface properties and biocompatibility are enhanced. Polymers are used in many biomedical applications; such as implants, because of their variable bulk properties. On the other hand, their surface properties are inadequate which demands certain surface treatments including atmospheric pressure plasma treatment. In biomedical applications, surface treatment is important to promote good cell adhesion, proliferation, and growth. This article aim is to give an overview of different atmospheric pressure plasma treatments of polymer surface, and their influence on cell-material interaction with different cell lines.Article Citation - WoS: 13Citation - Scopus: 14Core/Shell Glycine-Polyvinyl Alcohol/Polycaprolactone Nanofibrous Membrane Intended for Guided Bone Regeneration: Development and Characterization(Mdpi, 2021) Alazzawi, Marwa; Alsahib, Nabeel Kadim Abid; Sasmazel, Hilal TurkogluGlycine (Gly), which is the simplest amino acid, induces the inflammation response and enhances bone mass density, and particularly its beta polymorph has superior mechanical and piezoelectric properties. Therefore, electrospinning of Gly with any polymer, including polyvinyl alcohol (PVA), has a great potential in biomedical applications, such as guided bone regeneration (GBR) application. However, their application is limited due to a fast degradation rate and undesirable mechanical and physical properties. Therefore, encapsulation of Gly and PVA fiber within a poly(epsilon-caprolactone) (PCL) shell provides a slower degradation rate and improves the mechanical, chemical, and physical properties. A membrane intended for GBR application is a barrier membrane used to guide alveolar bone regeneration by preventing fast-proliferating cells from growing into the bone defect site. In the present work, a core/shell nanofibrous membrane, composed of PCL as shell and PVA:Gly as core, was developed utilizing the coaxial electrospinning technique and characterized morphologically, mechanically, physically, chemically, and thermally. Moreover, the characterization results of the core/shell membrane were compared to monolithic electrospun PCL, PVA, and PVA:Gly fibrous membranes. The results showed that the core-shell membrane appears to be a good candidate for GBR application with a nano-scale fiber of 412 +/- 82 nm and microscale pore size of 6.803 +/- 0.035 mu m. Moreover, the wettability of 47.4 +/- 2.2 degrees contact angle (C.A) and mechanical properties of 135 +/- 3.05 MPa average modulus of elasticity, 4.57 +/- 0.04 MPa average ultimate tensile stress (UTS), and 39.43% +/- 0.58% average elongation at break are desirable and suitable for GBR application. Furthermore, the X-ray diffraction (XRD) and transmission electron microscopy (TEM) results exhibited the formation of beta-Gly.Article Citation - WoS: 4Citation - Scopus: 5In Vitro Evaluation of Tooth-Colored Yttria Stabilized Zirconia Ceramics(Taylor & Francis Ltd, 2021) Akarsu, Melis Kaplan; Basar, Ahmet Ozan; Sasmazel, Hilal Turkoglu; Park, Jongee; Ozturk, AbdullahEffects of MoCl3 and NiCl2, originally incorporated as coloring agent, on the cellular response of 3 mol% yttria stabilized zirconia (3Y-TZP) ceramics was investigated. MoCl3 and NiCl2-MoCl3 incorporated, tooth-colored 3Y-TZP ceramics were produced through cold isostatic pressing at 100 MPa followed by pressureless sintering at 1450 degrees C for 2 h. Aging was performed on the sintered ceramics using distilled water in a reactor at 134 degrees C at 2.3 bar pressure for 2 h. The phases developed during different stages of processing were identified by X-ray diffraction (XRD) analysis. In vitro cell culture studies were carried out using L929 fibroblast cell line. The cell viability and proliferation studies revealed that none of the specimens showed cytotoxicity with respect to coloring. Confocal laser scanning microscope (CLSM) analyses suggested that all of the specimens exhibited good in vitro cytocompatibility. Enhancement in cell attachment, adhesion, and proliferation was observed in all specimens via scanning electron microscope (SEM) analysis. Although the coloring process did not improve the proliferation performance of the aged specimens, the incorporation of transition metals enhanced the in vitro performance of 3Y-TZP ceramics.Article Citation - Scopus: 1Biocompatibility of Electrospun Pva-Based Nanocomposite With Chemical Vapor Deposition-Derived Graphene Monolayer(Lukasiewicz Research Network-industrial Chemistry inst, 2024) Sasmazel, Hilal Turkoglu; Alazzawi, Marwa; Sadhu, Verra; Gozutok, MelikeThe biocompatibility of electrospun PVA with monolayer graphene obtained by chemical vapor deposition (PVA/CVD-grown MLG) nanocomposite was investigated. The properties of PVA/ CVD-grown MLG nanocomposite were compared with those of electrospun PVA mat. Raman analysis confirmed the presence of graphene monolayer on PVA. Although no significant changes in tensile properties were observed, the electrical conductivity increased from 0.1 (PVA mat) to 0.4 mu S/cm (PVA/ CVD-grown MLG). Thermal stability was also increased, as evidenced by the higher onset temperature and temperature of maximum decomposition rate determined by TGA. The contact angle decreased slightly, which resulted in higher PBS absorption and degradation of the nanocomposite. Water vapor transmission rate (WVTR) decreased from 40 (PVA mat) to 37 g/m2 h (PVA/CVD-grown MLG). Cell culture studies showed better cell viability, population, and growth in the case of PVA/CVD-grown MLG nanocomposite due to improved physical, chemical and mechanical properties.Article Citation - WoS: 34Citation - Scopus: 49Encapsulation of Β-Carotene by Emulsion Electrospraying Using Deep Eutectic Solvents(Mdpi, 2020) Ozan Basar, Ahmet; Prieto, Cristina; Durand, Erwann; Villeneuve, Pierre; Sasmazel, Hilal Turkoglu; Lagaron, JoseThe encapsulation beta-carotene in whey protein concentrate (WPC) capsules through the emulsion electrospraying technique was studied, using deep eutectic solvents (DES) as solvents. These novel solvents are characterized by negligible volatility, a liquid state far below 0 degrees C, a broad range of polarity, high solubilization power strength for a wide range of compounds, especially poorly water-soluble compounds, high extraction ability, and high stabilization ability for some natural products. Four DES formulations were used, based on mixtures of choline chloride with water, propanediol, glucose, glycerol, or butanediol. beta-Carotene was successfully encapsulated in a solubilized form within WPC capsules; as a DES formulation with choline chloride and butanediol, the formulation produced capsules with the highest carotenoid loading capacity. SEM micrographs demonstrated that round and smooth capsules with sizes around 2 mu m were obtained. ATR-FTIR results showed the presence of DES in the WPC capsules, which indirectly anticipated the presence of beta-carotene in the WPC capsules. Stability against photo-oxidation studies confirmed the expected presence of the bioactive and revealed that solubilized beta-carotene loaded WPC capsules presented excellent photo-oxidation stability compared with free beta-carotene. The capsules developed here clearly show the significant potential of the combination of DES and electrospraying for the encapsulation and stabilization of highly insoluble bioactive compounds.Article Citation - WoS: 27Citation - Scopus: 33Manufacturing of Zinc Oxide Nanoparticle (zno Np)-Loaded Polyvinyl Alcohol (pva) Nanostructured Mats Using ginger Extract for Tissue Engineering Applications(Mdpi, 2022) Izgis, Hursima; Ilhan, Elif; Kalkandelen, Cevriye; Celen, Emrah; Guncu, Mehmet Mucahit; Sasmazel, Hilal Turkoglu; Constantinescu, GabrielIn this research, as an alternative to chemical and physical methods, environmentally and cost-effective antimicrobial zinc oxide nanoparticles (ZnO NP) were produced by the green synthesis method. The current study focuses on the production of ZnO NP starting from adequate precursor and Zingiber officinale aqueous root extracts (ginger). The produced ZnO NP was loaded into electrospun nanofibers at different concentrations for various tissue engineering applications such as wound dressings. The produced ZnO NPs and ZnO NP-loaded nanofibers were examined by Scanning Electron Microscopy (SEM) for morphological assessments and Fourier-transform infrared spectrum (FT-IR) for chemical assessments. The disc diffusion method was used to test the antimicrobial activity of ZnO NP and ZnO NP-loaded nanofibers against three representatives strains, Escherichia coli (Gram-negative bacteria), Staphylococcus aureus (Gram-positive bacteria), and Candida albicans (fungi) microorganisms. The strength and stretching of the produced fibers were assessed using tensile tests. Since water absorption and weight loss behaviors are very important in tissue engineering applications, swelling and degradation analyses were applied to the produced nanofibers. Finally, the MTT test was applied to analyze biocompatibility. According to the findings, ZnO NP-loaded nanofibers were successfully synthesized using a green precipitation approach and can be employed in tissue engineering applications such as wound dressing.Editorial Editorial: Biofabricated Materials for Tissue Engineering(Frontiers Media Sa, 2024) Sasmazel, Hilal Turkoglu; Gunduz, Oguzhan; Ramalingam, Murugan; Ulag, Songul[No Abstract Available]
