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Article Citation - WoS: 22Citation - Scopus: 28Modeling of Dielectrophoretic Particle Motion: Point Particle Versus Finite-Sized Particle(Wiley, 2017) Cetin, Barbaros; Oner, S. Dogan; Baranoglu, BesimDielectrophoresis (DEP) is a very popular technique for microfluidic bio-particle manipulation. For the design of a DEP-based microfluidic device, simulation of the particle trajectory within the microchannel network is crucial. There are basically two approaches: (i) point-particle approach and (ii) finite-sized particle approach. In this study, many aspects of both approaches are discussed for the simulation of direct current DEP, alternating current DEP, and traveling-wave DEP applications. Point-particle approach is implemented using Lagrangian tracking method, and finite-sized particle is implemented using boundary element method. The comparison of the point-particle approach and finite-sized particle approach is presented for different DEP applications. Moreover, the effect of particle-particle interaction is explored by simulating the motion of closely packed multiple particles for the same applications, and anomalous-DEP, which is a result of particle-wall interaction at the close vicinity of electrode surface, is illustrated.Article Citation - WoS: 5Citation - Scopus: 5Performance Comparison of Aptamer- and Antibody-Based Biosensors for Bacteria Detection on Glass Surfaces(Taylor & Francis inc, 2024) Balci, Oguz; Kurekci, Asli; Ozalp, V. Cengiz; Cetin, BarbarosAntibodies are the most common ligands in commercial and research assay systems for detecting whole pathogen cells. On the other hand, aptamers are superior ligands with many advantages over antibodies in sensitive and robust assay development. Extensive comparisons between aptamer-based biosensors and immunosensors are limited to protein analytes. Here, we report a comparison of ligands (four antibodies and one aptamer for each bacteria) to be used as a biosensor for Escherichia coli and Staphylococcus aureus on glass surfaces through systematic experiments. We have demonstrated that anti-E. coli antibody and mouse monoclonal to S. aureus have the best performance among the compared ligands. Hence, the ligands with the best performance were further investigated within the scope of linear range, analytical sensitivity, and reproducibility of the results. We have demonstrated that anti-E. coli antibody with a capture efficiency of 89.1% and mouse monoclonal to S. aureus with a capture efficiency of 88.2% have the best performance among the compared ligands. The results suggest that antibody ligands function with higher efficiency than aptamer ligands but aptamers have strong potential as an analytical tool.
