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  • Article
    Citation - Scopus: 12
    Classification of parasite egg cells using gray level cooccurence matrix and kNN
    (Scientific Publishers of India, 2016) Şengül,G.
    Parasite eggs are around 20 to 80 μm dimensions, and they can be seen under microscopes only and their detection requires visual analyses of microscopic images, which requires human expertise and long analysis time. Besides visual analysis is very error prone to human procedures. In order to automatize this process, a number of studies are proposed in the literature. But there is still a gap between the preferred performance and the reported ones and it is necessary to increase the performance of the automatic parasite egg classification approaches. In this study a learning based statistical pattern recognition approach for parasite egg classification is proposed that will both decrease the time required for the manual classification by an expert and increase the performance of the previously suggested automated parasite egg classification approaches. The proposed method uses Gray-Level Co-occurrence Matrix as the feature extractor, which is a texture based statistical method that can differentiate the parasite egg cells based on their textures, and the k-Nearest Neighbourhood (kNN) classifier for the classification. The proposed method is tested on 14 parasite egg types commonly seen in humans. The results show that proposed method can classify the parasite egg cells with a performance rate of 99%. © 2016, Scientific Publishers of India. All rights reserved.
  • Article
    Citation - Scopus: 1
    Computer Vision Based Automated Cell Counting Pipeline: a Case Study for Hl60 Cancer Cell on Hemocytometer
    (Scientific Publishers of India, 2018) Özkan,A.; İşgör,S.B.; Şengül,G.; İşgör,Y.G.
    Counting of cells can give useful information about the cell density to understand the concerning cell culture condition. Usually, cell counting can be achieved manually with the help of the microscope and hemocytometer by the domain experts. The main drawback of the manual counting procedure is that the reliability highly depends on the experience and concentration of the examiners. Therefore, computer vision based automated cell counting is an essential tool to improve the accuracy. Although the commercial automated cell counting systems are available in the literature, their high cost limits their broader usage. In this study, we present a cell counting pipeline for light microscope images based on hemocytometer that can be easily adapted to the various cell types. The proposed method is robust to adverse image and cell culture conditions such as cell shape deformations, lightning conditions and brightness differences. In addition, we collect a novel human promyelocytic leukemia (HL60) cancer cell dataset to test our pipeline. The experimental results are presented in three measures: recall, precision and F-measure. The method reaches up to 98%, 92%, and 95% based on these three measures respectively by combining Support Vector Machine (SVM) and Histogram of Oriented Gradient (HOG). © 2018, Scientific Publishers of India. All rights reserved.