Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

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dc.authoridköse, sevil/0000-0003-2188-9534
dc.authoridOnen, Selin/0000-0002-3255-3035
dc.authoridKORKUSUZ, PETEK/0000-0002-7553-3915
dc.authorscopusid6602104436
dc.authorscopusid56494407000
dc.authorscopusid57191626691
dc.authorscopusid57204933836
dc.authorwosidköse, sevil/ABI-5227-2020
dc.authorwosidYersal, Nilgun/JCD-4862-2023
dc.authorwosidOnen, Selin/IWU-4803-2023
dc.contributor.authorKorkusuz, Petek
dc.contributor.authorKose, Sevil
dc.contributor.authorYersal, Nilgun
dc.contributor.authorOnen, Selin
dc.contributor.otherBasic Sciences
dc.contributor.otherNutrition and Dietetics
dc.date.accessioned2024-07-05T15:28:24Z
dc.date.available2024-07-05T15:28:24Z
dc.date.issued2019
dc.departmentAtılım Universityen_US
dc.department-temp[Korkusuz, Petek; Yersal, Nilgun] Hacettepe Univ, Fac Med, Dept Histol & Embryol, Ankara, Turkey; [Kose, Sevil] Atilim Univ, Fac Hlth Sci, Dept Nutr & Dietet Ankara, Ankara, Turkey; [Onen, Selin] Hacettepe Univ, Inst Hlth Sci, Dept Stem Cell Sci, Ankara, Turkeyen_US
dc.descriptionköse, sevil/0000-0003-2188-9534; Onen, Selin/0000-0002-3255-3035; KORKUSUZ, PETEK/0000-0002-7553-3915en_US
dc.description.abstractMagnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.en_US
dc.description.sponsorshipTechnical and Research Council of Turkey [113S819]; Hacettepe University Research Funds [013D04101005, THD-2017-13430]en_US
dc.description.sponsorshipThe experiments presented in this protocol chapter were supported by grants from Technical and Research Council of Turkey (#113S819) and Hacettepe University Research Funds (#013D04101005, #THD-2017-13430).en_US
dc.identifier.citation4
dc.identifier.doi10.1007/7651_2018_151
dc.identifier.endpage163en_US
dc.identifier.isbn9781493988709
dc.identifier.isbn9781493988693
dc.identifier.issn1064-3745
dc.identifier.issn1940-6029
dc.identifier.pmid30306535
dc.identifier.scopus2-s2.0-85060623062
dc.identifier.scopusqualityQ4
dc.identifier.startpage153en_US
dc.identifier.urihttps://doi.org/10.1007/7651_2018_151
dc.identifier.urihttps://hdl.handle.net/20.500.14411/2794
dc.identifier.volume1879en_US
dc.identifier.wosWOS:000894117800014
dc.institutionauthorKöse, Sevil
dc.institutionauthorÖnen, Selin
dc.language.isoenen_US
dc.publisherHumana Press incen_US
dc.relation.ispartofseriesMethods in Molecular Biology
dc.relation.publicationcategoryKitap Bölümü - Uluslararasıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHematopoietic stem cellen_US
dc.subjectMACSen_US
dc.subjectSkin stem cellen_US
dc.subjectSpermatogonial stem cellen_US
dc.titleMagnetic-Based Cell Isolation Technique for the Selection of Stem Cellsen_US
dc.typeBook Parten_US
dspace.entity.typePublication
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relation.isAuthorOfPublication71ff6244-19b2-496c-9b5d-a5dfea4572a5
relation.isAuthorOfPublication.latestForDiscoverya114276c-9b58-4517-9fe0-170c501e8909
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