Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

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Date

2019

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Publisher

Humana Press inc

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Basic Sciences
(2019)
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Nutrition and Dietetics
(2017)
Student admission to the Atılım University Department of Nutrition and Dietetics started in 2017. Our Department is the only academic institution to offer undergraduate-level education completely in English in the field of Nutrition and Dietetics in Ankara. The studies of our department may be classified into two main categories; education and research. The current education programs are offered taking into consideration the awareness of the responsibility in offering a degree in Nutrition and Dietetics; by competent instructors in the field, and with an inter-disciplinary approach. Our aim for the future alumni of our undergraduate program is to undertake their responsibilities in the light of their information with a professional insight, and the confidence to constantly update themselves at hospitals, polyclinics, public health centers, ministries, catering institutions, food companies, universities and such where they may be employed in positions such as health care professionals, academicians, researchers, directors or policy makers.

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Abstract

Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.

Description

köse, sevil/0000-0003-2188-9534; Onen, Selin/0000-0002-3255-3035; KORKUSUZ, PETEK/0000-0002-7553-3915

Keywords

Hematopoietic stem cell, MACS, Skin stem cell, Spermatogonial stem cell

Turkish CoHE Thesis Center URL

Citation

4

WoS Q

Scopus Q

Q4

Source

Volume

1879

Issue

Start Page

153

End Page

163

URI

https://doi.org/10.1007/7651_2018_151
 https://hdl.handle.net/20.500.14411/2794

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