Quartz Crystal Microbalance-Based Aptasensor Integrated With Magnetic Pre-Concentration System for Detection of <i>listeria Monocytogenes</I> in Food Samples

dc.authorscopusid 8547022700
dc.authorscopusid 7005367189
dc.authorscopusid 55453551500
dc.authorscopusid 58970526100
dc.authorscopusid 6701765135
dc.contributor.author Beyazit, Fatma
dc.contributor.author Arica, Mehmet Yakup
dc.contributor.author Acikgoz-Erkaya, Ilkay
dc.contributor.author Ozalp, Veli Cengiz
dc.contributor.author Bayramoglu, Gulay
dc.contributor.other Basic Sciences
dc.date.accessioned 2024-07-05T15:22:45Z
dc.date.available 2024-07-05T15:22:45Z
dc.date.issued 2024
dc.department Atılım University en_US
dc.department-temp [Beyazit, Fatma] Canakkale Onsekiz Mart Univ, Fac Med, Dept Obstet & Gynecol, Canakkale, Turkiye; [Arica, Mehmet Yakup; Bayramoglu, Gulay] Gazi Univ, Biochem Proc & Biomat Res Lab, TR-06500 Ankara, Turkiye; [Acikgoz-Erkaya, Ilkay] Ahi Evran Univ, Fac Engn & Architecture, Dept Environm Engn, Kirsehir, Turkiye; [Ozalp, Veli Cengiz] Atilim Univ, Sch Med, Dept Med Biol, Ankara, Turkiye; [Bayramoglu, Gulay] Gazi Univ, Fac Sci, Dept Chem, TR-06500 Ankara, Turkiye en_US
dc.description.abstract A fast and accurate identification of Listeria monocytogenes. A new quartz crystal microbalance (QCM) aptasensor was designed for the specific and rapid detection of L. monocytogenes. Before detection of the target bacterium from samples in the QCM aptasensor, a magnetic pre-enrichment system was used to eliminate any contaminant in the samples. The prepared magnetic system was characterized using ATR-FTIR, SEM, VSM, BET, and analytical methods. The saturation magnetization values of the Fe3O4, Fe3O4@PDA, and Fe3O4@PDA@DAPEG particles were 57.2, 40.8, and 36.4 emu/g, respectively. The same aptamer was also immobilized on the QCM crystal integrated into QCM flow cell and utilized to quantitatively detect L. monocytogenes cells from the samples. It was found that a specific aptamer-magnetic pre-concentration system efficiently captured L. monocytogenes cells in a short time (approximately 10 min). The Fe3O4@PDA@DA-PEG-Apt particles provided selective isolation of L. monocytogenes from the bacteria-spiked media up to 91.8%. The immobilized aptamer content of the magnetic particles was 5834 mu g/g using 500 ng Apt/mL. The QCM aptasensor showed a very high range of analytical performance to the target bacterium from 1.0 x 10(2) and 1.0 x 10(7) CFU/mL. The limit of detection (LOD) and limit of quantitation (LOQ) were 148 and 448 CFU/mL, respectively, from the feeding of the QCM aptasensor flow cell with the eluent of the magnetic pre-concentration system. The reproducibility of the aptasensor was more than 95%. The aptasensor was very specific to L. monocytogenes compared to the other Listeria species (i.e., L. ivanovii, L. innocua, and L. seeligeri) or other tested bacteria such as Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. The QCM aptasensor was regenerated with NaOH solution, and the system was reused many times. en_US
dc.description.sponsorship Scientific and Technological Research Council of Turkiye (TUBITAK) [119Z886] en_US
dc.description.sponsorship GB and VCO wish to acknowledge the support of the Scientific and Technological Research Council of Turkiye (TUBITAK) for this work (Project No: 119Z886). All the other authors acknowledge their respective universities and departments for their support during the preparation of this manuscript. en_US
dc.identifier.citationcount 0
dc.identifier.doi 10.1007/s00604-024-06307-2
dc.identifier.issn 0026-3672
dc.identifier.issn 1436-5073
dc.identifier.issue 5 en_US
dc.identifier.pmid 38570380
dc.identifier.scopus 2-s2.0-85189440574
dc.identifier.uri https://doi.org/10.1007/s00604-024-06307-2
dc.identifier.uri https://hdl.handle.net/20.500.14411/2237
dc.identifier.volume 191 en_US
dc.identifier.wos WOS:001197236200006
dc.institutionauthor Özalp, Veli Cengiz
dc.language.iso en en_US
dc.publisher Springer Wien en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 9
dc.subject Magnetic particles en_US
dc.subject Pre-concentration en_US
dc.subject Aptasensor en_US
dc.subject Quartz crystal microbalance en_US
dc.subject Listeria monocytogenes detection en_US
dc.title Quartz Crystal Microbalance-Based Aptasensor Integrated With Magnetic Pre-Concentration System for Detection of <i>listeria Monocytogenes</I> in Food Samples en_US
dc.type Article en_US
dc.wos.citedbyCount 7
dspace.entity.type Publication
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relation.isAuthorOfPublication.latestForDiscovery 5698576b-38e7-4cc8-9551-3e06cf62ede8
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