Antioxidant Activity of Micractinium Sp. (Chlorophyta) Extracts Against H2O2 Induced Oxidative Stress in Human Breast Adenocarcinoma Cells

dc.authorid Sonmez, Cagla/0000-0002-5864-6379
dc.authorscopusid 57205393236
dc.authorscopusid 59415411000
dc.authorscopusid 38762052500
dc.authorscopusid 6701805729
dc.authorwosid Bulut, Onur/Aad-2996-2020
dc.authorwosid Sonmez, Cagla/M-4065-2019
dc.authorwosid Sonmez, Cagla/K-2744-2015
dc.contributor.author Bulut, Onur
dc.contributor.author Kose, Iskin Engin
dc.contributor.author Sonmez, Cagla
dc.contributor.author Oktem, Huseyin Avni
dc.date.accessioned 2025-05-05T19:05:51Z
dc.date.available 2025-05-05T19:05:51Z
dc.date.issued 2024
dc.department Atılım University en_US
dc.department-temp [Bulut, Onur] Konya Food & Agr Univ, Fac Engn & Architecture, Dept Bioengn, TR-42080 Konya, Turkiye; [Bulut, Onur; Kose, Iskin Engin; Oktem, Huseyin Avni] Middle East Tech Univ, Dept Biol Sci, Univ Mah,Dumlupinar Blvd 1, TR-06800 Ankara, Turkiye; [Sonmez, Cagla] Atılım Univ, Fac Med, Dept Med Biol, TR-06830 Ankara, Turkiye en_US
dc.description Sonmez, Cagla/0000-0002-5864-6379 en_US
dc.description.abstract In response to the growing demand for high-value bioactive compounds, microalgae cultivation has gained a significant acceleration in recent years. Among these compounds, antioxidants have emerged as essential constituents in the food, pharmaceutical, and cosmetics industries. This study focuses on Micractinium sp. ME05, a green microalgal strain previously isolated from hot springs flora in our laboratory. Micractinium sp. cells were extracted using six different solvents, and their antioxidant capacity, as well as total phenolic, flavonoid, and carotenoid contents were evaluated. The methanolic extracts demonstrated the highest antioxidant capacity, measuring 7.72 and 93.80 mu mol trolox equivalents g-1 dry weight (DW) according to the DPPH and FRAP assays, respectively. To further characterize the biochemical profile, reverse phase high-performance chromatography (RP-HPLC) was employed to quantify twelve different phenolics, including rutin, gallic acid, benzoic acid, cinnamic acid, and beta-carotene, in the microalgal extracts. Notably, the acetone extracts of Micractinium sp. grown mixotrophically contained a high amount of gallic acid (469.21 +/- 159.74 mu g g-1 DW), while 4-hydroxy benzoic acid (403.93 +/- 20.98 mu g g-1 DW) was the main phenolic compound in the methanolic extracts under heterotrophic cultivation. Moreover, extracts from Micractinium sp. exhibited remarkable cytoprotective activity by effectively inhibiting hydrogen peroxide-induced oxidative stress and cell death in human breast adenocarcinoma (MCF-7) cells. In conclusion, with its diverse biochemical composition and adaptability to different growth regimens, Micractinium sp. emerges as a robust candidate for mass cultivation in nutraceutical and food applications. en_US
dc.description.sponsorship Konya Food and Agriculture University [BAP-2019/0040]; Konya Food and Agriculture University (KFAU) Strategic Products Research and Development Center - KFAU Research Project en_US
dc.description.sponsorship We would like to thank Konya Food and Agriculture University (KFAU) Strategic Products Research and Development Center (SARGEM) for technical support with the HPLC analysis and KFAU Research and Development Center for Diagnostic Kits (KIT-ARGEM) for the use of the facilities. The MCF-7 cells are a kind gift of Hasan Huseyin Kazan. We specially appreciate Assoc. Prof. Dr. Okan Bulut from University of Alberta for his help and guidance on statistical analysis. This work was funded by KFAU Research Project BAP-2019/0040. en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.doi 10.1038/s41598-024-63960-6
dc.identifier.issn 2045-2322
dc.identifier.issue 1 en_US
dc.identifier.pmid 39528524
dc.identifier.scopus 2-s2.0-85209481370
dc.identifier.scopusquality Q1
dc.identifier.uri https://doi.org/10.1038/s41598-024-63960-6
dc.identifier.uri https://hdl.handle.net/20.500.14411/10544
dc.identifier.volume 14 en_US
dc.identifier.wos WOS:001353042700022
dc.identifier.wosquality Q1
dc.language.iso en en_US
dc.publisher Nature Portfolio en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 3
dc.subject Microalgae en_US
dc.subject Antioxidants en_US
dc.subject Gallic Acid en_US
dc.subject 4-Hydroxy Benzoic Acid en_US
dc.subject Oxidative Stress en_US
dc.subject Cytoprotective Effect en_US
dc.title Antioxidant Activity of Micractinium Sp. (Chlorophyta) Extracts Against H2O2 Induced Oxidative Stress in Human Breast Adenocarcinoma Cells en_US
dc.type Article en_US
dc.wos.citedbyCount 2
dspace.entity.type Publication

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