G-Csf Treatment of Healthy Pediatric Donors Affects Their Hematopoietic Microenvironment Through Changes in Bone Marrow Plasma Cytokines and Stromal Cells

dc.authorid Çetinkaya, Duygu Uçkan/0000-0003-3593-6493
dc.authorid köse, sevil/0000-0003-2188-9534
dc.authorid Cagnan, Ilgin/0000-0003-1255-2788
dc.authorid Aerts Kaya, Fatima/0000-0002-9583-8572
dc.authorid KILIC, Emine/0000-0003-2984-7638
dc.authorscopusid 36439180500
dc.authorscopusid 36828284500
dc.authorscopusid 56494407000
dc.authorscopusid 57213279193
dc.authorscopusid 54410197000
dc.authorscopusid 10338821200
dc.authorscopusid 10338821200
dc.authorwosid Çetinkaya, Duygu Uçkan/X-2067-2019
dc.authorwosid köse, sevil/ABI-5227-2020
dc.authorwosid Cagnan, Ilgin/AAB-2153-2021
dc.authorwosid Aerts Kaya, Fatima/ABG-1880-2020
dc.contributor.author Aerts-Kaya, Fatima
dc.contributor.author Kilic, Emine
dc.contributor.author Kose, Sevil
dc.contributor.author Aydin, Gozde
dc.contributor.author Cagnan, Ilgin
dc.contributor.author Kuskonmaz, Baris
dc.contributor.author Uckan-Cetinkaya, Duygu
dc.contributor.other Nutrition and Dietetics
dc.date.accessioned 2024-07-05T15:18:47Z
dc.date.available 2024-07-05T15:18:47Z
dc.date.issued 2021
dc.department Atılım University en_US
dc.department-temp [Aerts-Kaya, Fatima; Kose, Sevil; Aydin, Gozde; Cagnan, Ilgin; Uckan-Cetinkaya, Duygu] Hacettepe Univ, Grad Sch Hlth Sci, Dept Stem Cell Sci, Ankara, Turkey; [Aerts-Kaya, Fatima; Kilic, Emine; Kose, Sevil; Aydin, Gozde; Cagnan, Ilgin; Uckan-Cetinkaya, Duygu] Hacettepe Univ, Ctr Stem Cell Res & Dev, TR-06100 Ankara, Turkey; [Kilic, Emine] Hacettepe Teknokent, Hemosoft IT & Training Serv, Ankara, Turkey; [Kose, Sevil] Atilim Univ, Dept Med Biol, Fac Med, Ankara, Turkey; [Cagnan, Ilgin] Eastern Mediterranean Univ, Fac Arts & Sci, Dept Biol Sci, Famagusta, North Cyprus, Turkey; [Kuskonmaz, Baris; Uckan-Cetinkaya, Duygu] Hacettepe Univ, Fac Med, Dept Pediat, Div Hematol,Bone Marrow Transplantat Unit, Ankara, Turkey en_US
dc.description Çetinkaya, Duygu Uçkan/0000-0003-3593-6493; köse, sevil/0000-0003-2188-9534; Cagnan, Ilgin/0000-0003-1255-2788; Aerts Kaya, Fatima/0000-0002-9583-8572; KILIC, Emine/0000-0003-2984-7638 en_US
dc.description.abstract Although G-CSF mobilized peripheral blood stem cell (PBSC) transplantation is commonly used in adults, bone marrow (BM) is still the preferred stem cell source in pediatric stem cell transplantation. Despite the fact that G-CSF is increasingly being used to enhance the hematopoietic stem/progenitor cell (HSPC) yield in BM transplantation (G-BM), the direct effects of G-CSF on the pediatric BM microenvironment have never been investigated. The BM hematopoietic niche provides the physical space where the HSPCs reside. This BM niche regulates HSPC quiescence and proliferation through direct interactions with other niche cells, including Mesenchymal Stromal Cells (MSCs). These cells have been shown to secrete a wide range of hematopoietic cytokines (CKs) and growth factors (GFs) involved in differentiation, retention and homing of hematopoietic cells. Here, we assessed changes in the BM microenvironment by measuring levels of 48 different CKs and GFs in G-BM and control BM (C-BM) plasma from pediatric donors. In addition, the effect of G-CSF on cell numbers and characteristics of HSPCs and MSCs was assessed. IL-16, SCGF-b, MIP-1b (all >1000 pg/mL) and RANTES (>10.000 pg/mL) were highly expressed in healthy donor pediatric BM plasma. Levels of IL-3, IL-18, GROa, MCP-3 (p<0.05) were increased in G-BM, whereas levels of RANTES (p<0.001) decreased after G-CSF treatment. We found a negative correlation with increasing age for IL2-Ra and LIF (p<0.05). In addition, a concomitant increase in the number of both hematopoietic and fibroblast colony forming units was observed, indicating that G-CSF affects both HSPC and MSC numbers. In conclusion, G-CSF treatment of healthy pediatric donors affects the hematopoietic BM microenvironment by expansion of HSPC and MSC numbers and modifying local CK and GF levels. en_US
dc.description.sponsorship European Commission [FP7-HEALTH-2010-261387-CELL-PID]; Republic of Turkey Ministry of Development [2006-K120640-PEDISTEM] en_US
dc.description.sponsorship This work was supported by grants from the European Commission (FP7-HEALTH-2010-261387-CELL-PID) and the Republic of Turkey Ministry of Development (2006-K120640-PEDISTEM). en_US
dc.identifier.citationcount 2
dc.identifier.doi 10.1016/j.cyto.2020.155407
dc.identifier.issn 1043-4666
dc.identifier.issn 1096-0023
dc.identifier.pmid 33383380
dc.identifier.scopus 2-s2.0-85098739321
dc.identifier.scopusquality Q2
dc.identifier.uri https://doi.org/10.1016/j.cyto.2020.155407
dc.identifier.uri https://hdl.handle.net/20.500.14411/1906
dc.identifier.volume 139 en_US
dc.identifier.wos WOS:000615871500007
dc.identifier.wosquality Q2
dc.institutionauthor Köse, Sevil
dc.language.iso en en_US
dc.publisher Academic Press Ltd- Elsevier Science Ltd en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 6
dc.subject Bone marrow en_US
dc.subject Cytokines en_US
dc.subject Hematopoietic growth factors en_US
dc.subject Hematopoietic stem and progenitor cells en_US
dc.subject Mesenchymal stromal cells en_US
dc.title G-Csf Treatment of Healthy Pediatric Donors Affects Their Hematopoietic Microenvironment Through Changes in Bone Marrow Plasma Cytokines and Stromal Cells en_US
dc.type Article en_US
dc.wos.citedbyCount 5
dspace.entity.type Publication
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