Surface Microbiota and Associated Staphylococci of Houseflies (musca Domestica) Collected From Different Environmental Sources

dc.authorid Özalp, Veli Cengiz/0000-0002-7659-5990
dc.authorid Kavruk, Murat/0000-0001-5331-7253
dc.authorid Yurt, Mediha Nur Zafer/0000-0002-3064-3811
dc.authorid Acar, Elif Esma/0000-0002-6264-7550
dc.authorid CAN, Ozge/0000-0002-3203-645X
dc.authorscopusid 24921796800
dc.authorscopusid 6504450287
dc.authorscopusid 57443615500
dc.authorscopusid 57443813100
dc.authorscopusid 57209077519
dc.authorscopusid 57211080335
dc.authorscopusid 55279809300
dc.authorwosid Özalp, Veli Cengiz/B-2940-2009
dc.authorwosid Kavruk, Murat/O-6694-2016
dc.authorwosid Can, Özge/GLN-7377-2022
dc.contributor.author Sudagidan, Mert
dc.contributor.author Ozalp, Veli Cengiz
dc.contributor.author Can, Ozge
dc.contributor.author Eligul, Hakan
dc.contributor.author Yurt, Mediha Nur Zafer
dc.contributor.author Tasbasi, Behiye Busra
dc.contributor.author Kocak, Oner
dc.contributor.other Basic Sciences
dc.date.accessioned 2024-07-05T15:18:00Z
dc.date.available 2024-07-05T15:18:00Z
dc.date.issued 2022
dc.department Atılım University en_US
dc.department-temp [Sudagidan, Mert; Yurt, Mediha Nur Zafer; Tasbasi, Behiye Busra; Acar, Elif Esma] Konya Food & Agr Univ, KIT ARGEM R&D Ctr, TR-42080 Meram, Konya, Turkey; [Ozalp, Veli Cengiz] Atilim Univ, Med Sch, Dept Med Biol, TR-06830 Ankara, Turkey; [Can, Ozge] Zmir Inst Technol, Dept Bioengn, TR-35430 Izmir, Turkey; [Eligul, Hakan] Nat Conservat Ctr DKM, TR-06530 Ankara, Turkey; [Tasbasi, Behiye Busra] BBT Biotechnol, InnoPk, TR-42250 Selcuklu, Konya, Turkey; [Kavruk, Murat] Atilim Univ, Sch Hlth Sci, Dept Nutr & Dietet, TR-06830 Ankara, Turkey; [Kocak, Oner] Vektorsan Biocidal Test & Anal Lab, TR-06370 Ankara, Turkey en_US
dc.description Özalp, Veli Cengiz/0000-0002-7659-5990; Kavruk, Murat/0000-0001-5331-7253; Yurt, Mediha Nur Zafer/0000-0002-3064-3811; Acar, Elif Esma/0000-0002-6264-7550; CAN, Ozge/0000-0002-3203-645X en_US
dc.description.abstract Houseflies (Musca domestica) are important mechanical vectors for the transmission of pathogenic microorganisms. In this study, 129 houseflies (69 males and 60 females) were collected from 10 different environmental sources and a laboratory population was used. The surface microbiota of houseflies was identified by NextGeneration Sequencing. Staphylococci from the surfaces of houseflies were selectively isolated and their virulence genes, antibiotic susceptibilities, biofilm formation, and clonal relatedness were determined. Metagenomic analysis results demonstrated that Staphylococcus, Bacillus, and Enterococcus were mostly present on the surface of houseflies at the genus level. Additionally, the isolated 32 staphylococcal strains were identified as Staphylococcus sciuri (n = 11), S. saprophyticus (n = 9), S. arlettae (n = 6), S. xylosus (n = 4), S. epidermidis (n = 1) and S. gallinarum (n = 1). tetK, tetM, tetL, ermC, msrAB, and aad6 genes were found to carry by some of the staphylococcal strains. The strains were mostly resistant to oxacillin, penicillin, and erythromycin and three strains were multi-drug resistant. There was a statistical difference between housefly collection places and antibiotic resistance of isolated staphylococci to penicillin G, gentamicin, and erythromycin (p < 0.05). Biofilm test showed that 17 strains were strong biofilm formers, and it plays important role in the transmission of these bacteria on the surface of houseflies. Staphylococcal strains showed extracellular proteolytic and lipolytic activity in 31 and 12 strains, respectively. Closely related species were found in PFGE analysis from different environmental sources. By this study, surface microbiota and carriage of pathogenic staphylococci on the surfaces of houseflies and their virulence properties were elucidated. en_US
dc.identifier.citationcount 4
dc.identifier.doi 10.1016/j.micpath.2022.105439
dc.identifier.issn 0882-4010
dc.identifier.issn 1096-1208
dc.identifier.pmid 35139420
dc.identifier.scopus 2-s2.0-85124175619
dc.identifier.uri https://doi.org/10.1016/j.micpath.2022.105439
dc.identifier.uri https://hdl.handle.net/20.500.14411/1820
dc.identifier.volume 164 en_US
dc.identifier.wos WOS:000788140400003
dc.identifier.wosquality Q2
dc.institutionauthor Özalp, Veli Cengiz
dc.language.iso en en_US
dc.publisher Academic Press Ltd- Elsevier Science Ltd en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 7
dc.subject Musca domestica en_US
dc.subject Microbiota en_US
dc.subject Staphylococci en_US
dc.subject Antibiotic resistance en_US
dc.subject Biofilm en_US
dc.title Surface Microbiota and Associated Staphylococci of Houseflies (musca Domestica) Collected From Different Environmental Sources en_US
dc.type Article en_US
dc.wos.citedbyCount 7
dspace.entity.type Publication
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relation.isAuthorOfPublication.latestForDiscovery 5698576b-38e7-4cc8-9551-3e06cf62ede8
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relation.isOrgUnitOfPublication.latestForDiscovery c6d3b3b7-f103-4779-9789-92b2e2420f2d

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