Development of Electrochemical Aptasensors Detecting Phosphate Ions on Tmb Substrate With Epoxy-Based Mesoporous Silica Nanoparticles

dc.authorid Özalp, Veli Cengiz/0000-0002-7659-5990
dc.authorid Yilmaz, Mahmut/0000-0001-5793-0805
dc.authorscopusid 57390982300
dc.authorscopusid 6504450287
dc.authorscopusid 55738450300
dc.authorscopusid 24921796800
dc.authorscopusid 57195693468
dc.authorscopusid 57211791524
dc.authorscopusid 57211080335
dc.authorwosid Özalp, Veli Cengiz/B-2940-2009
dc.authorwosid Yilmaz, Mahmut/A-4984-2012
dc.authorwosid ŞEN, Fatih/O-9407-2016
dc.contributor.author Altuner, Elif Esra
dc.contributor.author Ozalp, Veli Cengiz
dc.contributor.author Yilmaz, M. Deniz
dc.contributor.author Sudagidan, Mert
dc.contributor.author Aygun, Aysenur
dc.contributor.author Acar, Elif Esma
dc.contributor.author Sen, Fatih
dc.contributor.other Basic Sciences
dc.date.accessioned 2024-07-05T15:18:34Z
dc.date.available 2024-07-05T15:18:34Z
dc.date.issued 2022
dc.department Atılım University en_US
dc.department-temp [Altuner, Elif Esra; Aygun, Aysenur; Sen, Fatih] Dumlupinar Univ, Dept Biochem, TR-43000 Kutahya, Turkey; [Ozalp, Veli Cengiz] Atilim Univ, Med Sch, Dept Med Biol, TR-06830 Ankara, Turkey; [Yilmaz, M. Deniz] Konya Food & Agr Univ, Fac Engn & Architecture, Dept Bioengn, TR-42080 Konya, Turkey; [Sudagidan, Mert; Acar, Elif Esma; Tasbasi, Behiye Busra] Konya Food & Agr Univ, R&D Ctr, KIT ARGEM, TR-42080 Konya, Turkey en_US
dc.description Özalp, Veli Cengiz/0000-0002-7659-5990; Yilmaz, Mahmut/0000-0001-5793-0805; en_US
dc.description.abstract This study, it is aimed to develop an electrochemical aptasensor that can detect phosphate ions using 3.3 & PRIME;5.5 & PRIME; tetramethylbenzidine (TMB). It is based on the principle of converting the binding affinity of the target molecule phosphate ion (PO43-) into an electrochemical signal with specific aptamer sequences for the aptasensor to be developed. The aptamer structure served as a gate for the TMB to be released and was used to trap the TMB molecule in mesoporous silica nanoparticles (MSNPs). The samples for this study were characterized by transmission electron spectroscopy (TEM), Brunner-Emmet-Teller, dynamic light scattering & electrophoretic light scattering, and induction coupled plasma atomic emission spectroscopy. According to TEM analysis, MSNPs have a morphologically hexagonal structure and an average size of 208 nm. In this study, palladium-carbon nano particles (Pd/C NPs) with catalytic reaction were used as an alternative to the biologically used horseradish peroxidase (HRP) enzyme for the release of TMB in the presence of phosphate ions. The limit of detection (LOD) was calculated as 0.983 mu M, the limit of determination (LOQ) was calculated as 3.276 mu M, and the dynamic linear phosphate range was found to be 50-1000 mu M. The most important advantage of this bio-based aptasensor assembly is that it does not contain molecules such as a protein that cannot be stored for a long time at room temperature, so its shelf life is very long compared to similar systems developed with antibodies. The proposed sensor shows good recovery in phosphate ion detection and is considered to have great potential among electrochemical sensors. en_US
dc.identifier.citationcount 16
dc.identifier.doi 10.1016/j.chemosphere.2022.134077
dc.identifier.issn 0045-6535
dc.identifier.issn 1879-1298
dc.identifier.pmid 35218784
dc.identifier.scopus 2-s2.0-85125434713
dc.identifier.uri https://doi.org/10.1016/j.chemosphere.2022.134077
dc.identifier.uri https://hdl.handle.net/20.500.14411/1864
dc.identifier.volume 297 en_US
dc.identifier.wos WOS:000784335400004
dc.identifier.wosquality Q1
dc.institutionauthor Özalp, Veli Cengiz
dc.language.iso en en_US
dc.publisher Pergamon-elsevier Science Ltd en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 25
dc.subject 3.3'5.5'tetra methyl benzidine en_US
dc.subject Aptamer en_US
dc.subject Aptasensor en_US
dc.subject Mesoporus silica nanoparticles en_US
dc.subject Phosphate ions en_US
dc.title Development of Electrochemical Aptasensors Detecting Phosphate Ions on Tmb Substrate With Epoxy-Based Mesoporous Silica Nanoparticles en_US
dc.type Article en_US
dc.wos.citedbyCount 23
dspace.entity.type Publication
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