Browsing by Author "Kalkandelen, Cevriye"
Now showing 1 - 4 of 4
- Results Per Page
- Sort Options
Article Citation Count: 63Coaxial and emulsion electrospinning of extracted hyaluronic acid and keratin based nanofibers for wound healing applications(Pergamon-elsevier Science Ltd, 2021) Şaşmazel, Hilal Türkoğlu; Bedir, Tuba; Kalkandelen, Cevriye; Basar, Ahmet Ozan; Sasmazel, Hilal Turkoglu; Ustundag, Cem Bulent; Gunduz, Oguzhan; Metallurgical and Materials EngineeringNovel composites based on poly(epsilon-caprolactone)/polyethylene oxide loaded with hyaluronic acid(HA) and keratin(KR) were produced separately using emulsion and coaxial electrospinning methods. HA and KR were extracted from animal sources, characterized and loaded into coaxial fiber structures as bioactive agents, separately and together. Morphological, chemical, thermal, and mechanical characteristics of the fibers were investigated. According to the SEM results, diameters of smooth and beadless fibers fabricated via emulsion method were at nanoscale (sub-micron) while fibers of coaxial method were at micro scale. Benefitted electrospinning techniques demonstrated that hydrophobic and hydrophilic polymers can be advantageously combined. Core polymer specific FT-IR bands were not visible, their presence was proven with DSC analysis which confirms core-shell morphology of the fibers. In vitro studies exhibited spun mats did not have any cytotoxic effects and the HA and KR incorporated into the fiber structure synergistically increased cell viability and cell proliferation. This study demonstrated that the electrospun fibers containing HA and KR fabricated by both emulsion and coaxial methods can be efficient for wound healing applications.Article Citation Count: 5A drug-eluting nanofibrous hyaluronic acid-keratin mat for diabetic wound dressing(Springernature, 2022) Şaşmazel, Hilal Türkoğlu; Bedir, Tuba; Kalkandelen, Cevriye; Sasmazel, Hilal Turkoglu; Basar, Ahmet Ozan; Chen, Jing; Gunduz, Oguzhan; Metallurgical and Materials EngineeringDiabetes mellitus is a chronic metabolic disease associated with long-term multisystem complications, among which are non-healing diabetic foot ulcers (DFUs). Electrospinning is a sophisticated technique for the preparation of polymeric nanofibers impregnated with drugs for wound healing, burns, and diabetic ulcers. This study describes the fabrication and characterization of a novel drug-eluting dressing made of core-shell structured hyaluronic acid (HA)-keratin (KR)-polyethylene oxide (PEO) and polycaprolactone (PCL) nanofibers to treat diabetic wounds. The core-shell nanofibers produced by the emulsion electrospinning technique provide loading of metformin hydrochloride (MH), HA, and KR in the core of nanofibers, which in return improves the sustained long term release of the drug and prolongs the bioactivity. Morphological and chemical properties of the fibers were examined by SEM, FTIR, and XRD studies. It was observed that the fibers which contain HA and KR showed thin fiber structure, greater swelling capacity, fast degradation and increased cumulative drug release amount than neat emulsion fibers due to the hydrophilic nature of HA and KR. MH showed a sustained release from all fiber samples over 20 days and followed the first-order and Higuchi model kinetics and Fickian diffusion mechanism according to kinetic analysis results. In vitro cell culture studies showed that the developed mats exhibited enhanced biocompatibility performance with HA and KR incorporation. The results show that HA and KR-based emulsion electrospun fiber mats are potentially useful new nanofiber-based biomaterials in their use as drug carriers to treat diabetic wounds.Article Citation Count: 9Manufacturing of Zinc Oxide Nanoparticle (ZnO NP)-Loaded Polyvinyl Alcohol (PVA) Nanostructured Mats Using Ginger Extract for Tissue Engineering Applications(Mdpi, 2022) Şaşmazel, Hilal Türkoğlu; Ilhan, Elif; Kalkandelen, Cevriye; Celen, Emrah; Guncu, Mehmet Mucahit; Sasmazel, Hilal Turkoglu; Constantinescu, Gabriel; Metallurgical and Materials EngineeringIn this research, as an alternative to chemical and physical methods, environmentally and cost-effective antimicrobial zinc oxide nanoparticles (ZnO NP) were produced by the green synthesis method. The current study focuses on the production of ZnO NP starting from adequate precursor and Zingiber officinale aqueous root extracts (ginger). The produced ZnO NP was loaded into electrospun nanofibers at different concentrations for various tissue engineering applications such as wound dressings. The produced ZnO NPs and ZnO NP-loaded nanofibers were examined by Scanning Electron Microscopy (SEM) for morphological assessments and Fourier-transform infrared spectrum (FT-IR) for chemical assessments. The disc diffusion method was used to test the antimicrobial activity of ZnO NP and ZnO NP-loaded nanofibers against three representatives strains, Escherichia coli (Gram-negative bacteria), Staphylococcus aureus (Gram-positive bacteria), and Candida albicans (fungi) microorganisms. The strength and stretching of the produced fibers were assessed using tensile tests. Since water absorption and weight loss behaviors are very important in tissue engineering applications, swelling and degradation analyses were applied to the produced nanofibers. Finally, the MTT test was applied to analyze biocompatibility. According to the findings, ZnO NP-loaded nanofibers were successfully synthesized using a green precipitation approach and can be employed in tissue engineering applications such as wound dressing.Article Citation Count: 1Physico-chemical characterization and in vitro biological study of manganese doped β-tricalcium phosphate-based ceramics for bone regeneration applications(Springer, 2023) Şaşmazel, Hilal Türkoğlu; Daglilar, Sibel; Kalkandelen, Cevriye; Balescu, Liliana-Marinela; Sasmazel, Hilal Turkoglu; Pasuk, Iuliana; Gunduz, Oguzhan; Metallurgical and Materials EngineeringThis work evaluates the effects of manganese (Mn) doping on the morpho-structural features, mechanical performance, and in vitro biological response of beta-tricalcium phosphate (beta-TCP) derived bioceramics for bone tissue engineering applications. Five different Mn doping levels (i.e., 0.01%, 0.05%, 0.1%, 0.5%, and 1 wt.%) were investigated, with the beta-TCP-based bioceramics being sintered at four temperatures (i.e., 1000, 1100, 1200, and 1300 degrees C). A densification improvement was induced when using Mn in excess of 0.05 wt.%; the densification remained stationary in the sintering temperature range of 1200 - 1300 degrees C. The structural analyses evidenced that all samples sintered at 1000 and 1100 degrees C were composed of beta-TCP as major phase and hydroxyapatite (HA) as a minor constituent (similar to 4-6 wt.%). At the higher temperatures (1200 and 1300 degrees C), the formation of alpha-TCP was signalled at the expense of both beta-TCP and HA. The Mn doping was evidenced by lattice parameters changes. The evolution of the phase weights is linked to a complex inter-play between the capacity of the compounds to incorporate Mn and the thermal decomposition kinetics. The Mn doping induced a reduction in the mechanical performance (in terms of compressive strength, Vickers hardness and elastic modulus) of the beta-TCP-based ceramics. The metabolic activity and viability of osteoblastic cells (MC3T3-E1) for the ceramics were studied in both powder and compacted pellet form. Ceramics with Mn doping levels lower than 0.1 wt.% yielded a more favorable microenvironment for the osteoblast cells with respect to the undoped beta-TCP. No cytotoxic effects were recorded up to 21 days. The Mn-doped beta-TCPs showed a significant increase (p < 0.01) in alkaline phosphatase activity with respect to pure beta-TCP.