Leptin Promotes Proliferation of Neonatal Mouse Stem/Progenitor Spermatogonia

dc.authorid köse, sevil/0000-0003-2188-9534
dc.authorid KORKUSUZ, PETEK/0000-0002-7553-3915
dc.authorid Horzum, Utku/0000-0002-6747-3043
dc.authorid Ozkavukcu, Sinan/0000-0003-4525-9027
dc.authorscopusid 57191626691
dc.authorscopusid 56494407000
dc.authorscopusid 56300653200
dc.authorscopusid 14010410700
dc.authorscopusid 6604065476
dc.authorscopusid 6602104436
dc.authorwosid KORKUSUZ, PETEK/I-8321-2013
dc.authorwosid köse, sevil/ABI-5227-2020
dc.authorwosid KORKUSUZ, PETEK/JCD-9195-2023
dc.authorwosid Yersal, Nilgun/JCD-4862-2023
dc.authorwosid Horzum, Utku/P-7360-2018
dc.authorwosid Ozkavukcu, Sinan/A-8802-2019
dc.contributor.author Yersal, Nilgun
dc.contributor.author Kose, Sevil
dc.contributor.author Horzum, Utku
dc.contributor.author Ozkavukcu, Sinan
dc.contributor.author Orwig, Kyle E.
dc.contributor.author Korkusuz, Petek
dc.contributor.other Nutrition and Dietetics
dc.date.accessioned 2024-07-05T15:39:26Z
dc.date.available 2024-07-05T15:39:26Z
dc.date.issued 2020
dc.department Atılım University en_US
dc.department-temp [Yersal, Nilgun; Korkusuz, Petek] Hacettepe Univ, Dept Histol & Embryol, Fac Med, Ankara, Turkey; [Kose, Sevil] Atilim Univ, Dept Med Biol, Fac Med, Ankara, Turkey; [Horzum, Utku] Hacettepe Univ, Dept Basic Oncol, Canc Inst, Ankara, Turkey; [Ozkavukcu, Sinan] Ankara Univ, Ctr Assisted Reprod, Dept Obstet & Gynecol, Fac Med, Ankara, Turkey; [Orwig, Kyle E.] Univ Pittsburgh, Magee Womens Res Inst, Dept Obstet Gynecol & Reprod Sci, Sch Med, Pittsburgh, PA 15213 USA en_US
dc.description köse, sevil/0000-0003-2188-9534; KORKUSUZ, PETEK/0000-0002-7553-3915; Horzum, Utku/0000-0002-6747-3043; Ozkavukcu, Sinan/0000-0003-4525-9027 en_US
dc.description.abstract Purpose To keep and increase spermatogonial stem cell number (SSC) is the only available option for pediatric cancer survivors to maintain fertility. Leptin is secreted by the epididymal white adipose tissue and has receptors on stem/progenitor spermatogonia. The purpose of this study is to demonstrate dose- and time-dependent proliferative effect of leptin on stem/progenitor spermatogonia cultures from prepubertal mice testes. Methods CD90.2 (+) stem/progenitor spermatogonia were isolated from the C57BL/6 mouse testis on postnatal day 6 and placed in culture. The proliferative effect of leptin supplementation was assessed by colony formation (diameter and number), WST proliferation assays, and xCELLigence real-time cell analysis (RTCA) on days 3, 5, and 7 of culture. Expressions of p-ERK1/2, p-STAT3, total STAT3, and p-SHP2 levels were determined by western blot analysis. Results Leptin supplementation of 100 ng/ml increased the diameter (p= 0.001) and number (p= 0.01) of colonies in stem/progenitor spermatogonial cultures and caused higher proliferation by WST-1 (p= 0.009) compared with the control on day 7. The EC50 was calculated as 114 ng/ml for leptin by RTCA. Proliferative dose of leptin induced increased expression of p-ERK1/2 (p= 0.009) and p-STAT3 (p= 0.023) on stem/progenitor spermatogonia when compared with the untreated group. Conclusion The results indicated that leptin supplementation exhibited a dose- and time-dependent proliferative effect on stem/progenitor spermatogonia that was associated with increased expression of ERK1/2 and STAT3 pathways while maintaining their undifferentiated state. This output presents a new agent that may help to expand the stem/progenitor spermatogonia pool from the neonatal testis in order to autotransplant after cancer treatment. en_US
dc.description.sponsorship Research Coordination Unit of Hacettepe University [THD-2017-13430]; Eunice Kennedy Shriver National Institute of Child Health and Human Development [HD092084] en_US
dc.description.sponsorship The Research Coordination Unit of Hacettepe University funded this work (THD-2017-13430). Professor Kyle E Orwig was also supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development grant HD092084. en_US
dc.identifier.citationcount 10
dc.identifier.doi 10.1007/s10815-020-01929-w
dc.identifier.endpage 2838 en_US
dc.identifier.issn 1058-0468
dc.identifier.issn 1573-7330
dc.identifier.issue 11 en_US
dc.identifier.pmid 32840762
dc.identifier.scopus 2-s2.0-85089869229
dc.identifier.scopusquality Q2
dc.identifier.startpage 2825 en_US
dc.identifier.uri https://doi.org/10.1007/s10815-020-01929-w
dc.identifier.uri https://hdl.handle.net/20.500.14411/3226
dc.identifier.volume 37 en_US
dc.identifier.wos WOS:000562979000002
dc.identifier.wosquality Q2
dc.institutionauthor Köse, Sevil
dc.language.iso en en_US
dc.publisher Springer/plenum Publishers en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 14
dc.subject Stem en_US
dc.subject progenitor spermatogonia en_US
dc.subject Leptin en_US
dc.subject Proliferation en_US
dc.subject p-STAT3 en_US
dc.subject p-ERK1 en_US
dc.subject 2 en_US
dc.title Leptin Promotes Proliferation of Neonatal Mouse Stem/Progenitor Spermatogonia en_US
dc.type Article en_US
dc.wos.citedbyCount 13
dspace.entity.type Publication
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