Cellular Defense Enzyme Profile for Non-Cytotoxic and Phenol Enriched Extracts of <i>heliotropium Europaeum</I>, <i>carlina Oligocephala</I> and <i>echinops Ritro</I>

dc.authorid ISGOR, Belgin S/0000-0001-5716-3159
dc.authorid Isgor, Yasemin G./0000-0002-6021-257X
dc.authorscopusid 57190741107
dc.authorscopusid 7801688219
dc.authorscopusid 19638724400
dc.authorwosid ISGOR, Belgin S/B-7829-2013
dc.authorwosid Isgor, Yasemin G./AAE-4859-2021
dc.authorwosid Isgor, Yasemin G./B-3322-2010
dc.contributor.author Isgor, Belgin S.
dc.contributor.author İşgör, Sultan Belgin
dc.contributor.author Isgor, Yasemin G.
dc.contributor.author Geven, Fatmagul
dc.contributor.author İşgör, Sultan Belgin
dc.contributor.other Chemical Engineering
dc.contributor.other Chemical Engineering
dc.date.accessioned 2024-07-05T15:29:55Z
dc.date.available 2024-07-05T15:29:55Z
dc.date.issued 2018
dc.department Atılım University en_US
dc.department-temp [Isgor, Belgin S.] Atilim Univ, Fac Engn, Dept Chem Engn & Appl Chem, Ankara, Turkey; [Isgor, Yasemin G.] Ankara Univ, Vocat Sch Hlth Sci, Med Lab Tech Program, Kecioren Campus,Fatih Cad 197-A, Ankara, Turkey; [Geven, Fatmagul] Ankara Univ, Fac Sci, Dept Biol, Ankara, Turkey en_US
dc.description ISGOR, Belgin S/0000-0001-5716-3159; Isgor, Yasemin G./0000-0002-6021-257X en_US
dc.description.abstract Background and Objective:The discovery of traditional plants with some medicinal properties, verifying their biological targets and the bioassay guided standardization of their active components are the particular interest of diverse research groups recently. These efforts may help to revise the therapy modalities with natural product supplements. In this context, the possible biological targets of plants, namely Heliotropium europaeum, Carlina oligocephala and Echinops ritro, with no known medicinal value but recognized for their region specific traditional use, were evaluated. Here, the biological targets were enzymes of the antioxidant and xenobiotic defense mechanisms, with roles on inflammatory response. Materials and Methods: Cytotoxicity analyses were performed by using human promyelocytic (HL60) and chronic myelogenous leukemia (K562) cells for circulating models and breastadenocarcinoma (MCF7) cells for epithelial model to evaluate the non-toxic dose range of extracts by virtue of XTT and trypan blue. The target aimed effectiveness of these plants were determined with dose response profiles and IC50 values against glutathione peroxidase (GPX), glutathione transferase(GST),catalase (CAT) and superoxide dismutase (SOD) targets, as well as their capacity to reduce free radicals (DPPH) and non-radical hydrogen peroxide (H2O2). For standardization purposes, all extract concentrations were brought to 23.81 mg L-1 GAE and dilutions were made from these stocks. The IC50 values were determined by nonlinear regression analysis, with sigmoidal dose-response 4-parameter logistic equation. Results: The results showed that the best DPPH and H2O2 scavenging was observed with E ritro extracts. On the contrary, the best enzyme inhibition profile was observed with H europaeum against CAT, SOD, GPX and GST targets. Among the enzymes evaluated, all plants with different fractions also exerted strong GPX and CAT inhibition. Conclusion: The enzyme profiling of extracts may reveal the medicinal value of herbal remedies, by identifying their effects on cellular targets. In addition to define how reasonable the use of plants in traditional and complementary medicine (TCM) practices,these efforts may help to improve the standardized supplement preparations to benefit therapeutics with reduced efficiency due to inference with cellular defense and drug resistance enzymes, or both. en_US
dc.description.sponsorship Atilim University Research grant [BAP-A-1112-02]; [TUBITAK-COST-109T548-2010] en_US
dc.description.sponsorship This study was performed at Atilim University Biochemistry Research Laboratory, partially funded by TUBITAK-COST-109T548-2010, Atilim University Research grant BAP-A-1112-02. Authors would like to thank Karl Machin for lingual. en_US
dc.identifier.citationcount 2
dc.identifier.doi 10.3923/ijp.2018.224.238
dc.identifier.endpage 238 en_US
dc.identifier.issn 1811-7775
dc.identifier.issn 1812-5700
dc.identifier.issue 2 en_US
dc.identifier.scopus 2-s2.0-85041344699
dc.identifier.startpage 224 en_US
dc.identifier.uri https://doi.org/10.3923/ijp.2018.224.238
dc.identifier.uri https://hdl.handle.net/20.500.14411/2948
dc.identifier.volume 14 en_US
dc.identifier.wos WOS:000419946100008
dc.identifier.wosquality Q4
dc.institutionauthor İşgör, Sultan Belgin
dc.language.iso en en_US
dc.publisher Asian Network Scientific information-ansinet en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 3
dc.subject Glutathione transferase en_US
dc.subject glutathione peroxidase en_US
dc.subject catalase en_US
dc.subject superoxide dismutase en_US
dc.subject cytosolic defense en_US
dc.subject anti-inflammatory effect en_US
dc.title Cellular Defense Enzyme Profile for Non-Cytotoxic and Phenol Enriched Extracts of <i>heliotropium Europaeum</I>, <i>carlina Oligocephala</I> and <i>echinops Ritro</I> en_US
dc.type Article en_US
dc.wos.citedbyCount 3
dspace.entity.type Publication
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