Bacterial Skin Microbiota of Seabass From Aegean Fish Farms and Antibiotic Susceptibility of Psychrotrophic <i>pseudomonas</I>

dc.authorid Zornu, Jacob/0000-0002-0154-2405
dc.authorid Özalp, Veli Cengiz/0000-0002-7659-5990
dc.authorid Aydin, Ali/0000-0002-9550-9111
dc.authorid Mamatova, Zhanylbubu/0000-0002-1763-5585
dc.authorid Yurt, Mediha Nur Zafer/0000-0002-3064-3811
dc.authorscopusid 7102287952
dc.authorscopusid 24921796800
dc.authorscopusid 58294063200
dc.authorscopusid 57209077519
dc.authorscopusid 6504450287
dc.authorscopusid 58293801800
dc.authorscopusid 6506202792
dc.authorwosid Zornu, Jacob/IQW-4860-2023
dc.authorwosid Özalp, Veli Cengiz/B-2940-2009
dc.authorwosid Aydin, Ali/ACM-7774-2022
dc.contributor.author Aydin, Ali
dc.contributor.author Sudagidan, Mert
dc.contributor.author Mamatova, Zhanylbubu
dc.contributor.author Yurt, Mediha Nur Zafer
dc.contributor.author Ozalp, Veli Cengiz
dc.contributor.author Zornu, Jacob
dc.contributor.author Brun, Edgar
dc.contributor.other Basic Sciences
dc.date.accessioned 2024-07-05T15:25:11Z
dc.date.available 2024-07-05T15:25:11Z
dc.date.issued 2023
dc.department Atılım University en_US
dc.department-temp [Aydin, Ali; Mamatova, Zhanylbubu] Istanbul Univ Cerrahpasa, Fac Vet Med, Dept Food Hyg & Technol, TR-34320 Istanbul, Turkiye; [Sudagidan, Mert; Yurt, Mediha Nur Zafer] Konya Food & Agr Univ, KIT ARGEM R&D Ctr, TR-42080 Konya, Turkiye; [Ozalp, Veli Cengiz] Atilim Univ, Med Sch, Dept Med Biol, TR-06830 Ankara, Turkiye; [Zornu, Jacob; Tavornpanich, Saraya; Brun, Edgar] Norwegian Vet Inst, N-1433 As, Norway en_US
dc.description Zornu, Jacob/0000-0002-0154-2405; Özalp, Veli Cengiz/0000-0002-7659-5990; Aydin, Ali/0000-0002-9550-9111; Mamatova, Zhanylbubu/0000-0002-1763-5585; Yurt, Mediha Nur Zafer/0000-0002-3064-3811 en_US
dc.description.abstract Farming seabass (Dicentrarchus labrax) is an essential activity in the Mediterranean basin including the Aegean Sea. The main seabass producer is Turkey accounting for 155,151 tons of production in 2021. In this study, skin swabs of seabass farmed in the Aegean Sea were analysed with regard to the isolation and identification of Pseudomonas. Bacterial microbiota of skin samples (n = 96) from 12 fish farms were investigated using next-generation sequencing (NGS) and metabarcoding analysis. The results demonstrated that Proteobacteria was the dominant bacterial phylum in all samples. At the species level, Pseudomonas lundensis was identified in all samples. Pseudomonas, Shewanella, and Flavobacterium were identified using conventional methods and a total of 46 viable (48% of all NGS+) Pseudomonas were isolated in seabass swab samples. Additionally, antibiotic susceptibility was determined according to standards of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) in psychrotrophic Pseudomonas. Pseudomonas strains were tested for susceptibility to 11 antibiotics (piperacillin-tazobactam, gentamicin, tobramycin, amikacin, doripenem, meropenem, imipenem, levofloxacin, ciprofloxacin, norfloxacin, and tetracycline) from five different groups of antibiotics (penicillins, aminoglycosides, carbapenems, fluoroquinolones, and tetracyclines). The antibiotics chosen were not specifically linked to usage by the aquaculture industry. According to the EUCAST and CLSI, three and two Pseudomonas strains were found to be resistant to doripenem and imipenem (E-test), respectively. All strains were susceptible to piperacillin-tazobactam, amikacin, levofloxacin, and tetracycline. Our data provide insight into different bacteria that are prevalent in the skin microbiota of seabass sampled from the Aegean Sea in Turkey, and into the antibiotic resistance of psychrotrophic Pseudomonas spp. en_US
dc.description.sponsorship Norwegian Veterinary Institute en_US
dc.description.sponsorship The authors would like to thank Peter PAULSEN for assistance with English editing, and Alper YANKIN for assistance with sampling. The authors would like to acknowledge Sezer OZKAN (Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa) and Berke TUMBAL (Department of Molecular Biology and Genetics, Istanbul Kultur University) for their technical assistance. Open Access Funding by the Norwegian Veterinary Institute. en_US
dc.identifier.citationcount 3
dc.identifier.doi 10.3390/foods12101956
dc.identifier.issn 2304-8158
dc.identifier.issue 10 en_US
dc.identifier.pmid 37238774
dc.identifier.scopus 2-s2.0-85160531978
dc.identifier.uri https://doi.org/10.3390/foods12101956
dc.identifier.uri https://hdl.handle.net/20.500.14411/2517
dc.identifier.volume 12 en_US
dc.identifier.wos WOS:000997099500001
dc.identifier.wosquality Q1
dc.institutionauthor Özalp, Veli Cengiz
dc.language.iso en en_US
dc.publisher Mdpi en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 7
dc.subject seabass en_US
dc.subject microbiota en_US
dc.subject fish farms en_US
dc.subject Pseudomonas en_US
dc.subject antibiotic resistance en_US
dc.title Bacterial Skin Microbiota of Seabass From Aegean Fish Farms and Antibiotic Susceptibility of Psychrotrophic <i>pseudomonas</I> en_US
dc.type Article en_US
dc.wos.citedbyCount 7
dspace.entity.type Publication
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relation.isAuthorOfPublication.latestForDiscovery 5698576b-38e7-4cc8-9551-3e06cf62ede8
relation.isOrgUnitOfPublication c6d3b3b7-f103-4779-9789-92b2e2420f2d
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