Effects of Nozzle Type Atmospheric Dry Air Plasma on L929 Fibroblast Cells Hybrid Poly (ε-caprolactone)/Chitosan (ε-Caprolactone) Scaffolds Interactions

dc.authorid Ozkan, Ozan/0000-0002-9050-1583
dc.authorid Turkoglu Sasmazel, Hilal/0000-0002-0254-4541
dc.authorscopusid 57189848353
dc.authorscopusid 16680382000
dc.contributor.author Ozkan, Ozan
dc.contributor.author Sasmazel, Hilal Turkoglu
dc.contributor.other Metallurgical and Materials Engineering
dc.date.accessioned 2024-07-05T14:31:02Z
dc.date.available 2024-07-05T14:31:02Z
dc.date.issued 2016
dc.department Atılım University en_US
dc.department-temp [Ozkan, Ozan; Sasmazel, Hilal Turkoglu] Atilim Univ, Dept Met & Mat Engn, TR-06836 Ankara, Turkey en_US
dc.description Ozkan, Ozan/0000-0002-9050-1583; Turkoglu Sasmazel, Hilal/0000-0002-0254-4541 en_US
dc.description.abstract In the study presented here, in order to improve the surface functionality and topography of poly (epsilon-caprolactone) (PCL)/chitosan/PCL hybrid tissue scaffolds fabricated layer by layer with electrospinning technique, an atmospheric pressure nozzle type plasma surface modification was utilized. The optimization of the plasma process parameters was carried out by monitoring the changes in surface hydrophilicity by using contact angle measurements. SEM, AFM and XPS analyses were utilized to observe the changes in topographical and chemical properties of the modified surfaces. The results showed that applied plasma modification altered the nanotopography and the functionality of the surfaces of the scaffolds. The modification applied for 9 min from a distance of 17 cm was found to provide the possible contact angle value (75.163 +/- 0.083) closest to the target value which is the value of tissue culture polystyrene (TCPS) petri dishes (similar to 49.7 degrees), compared to the unmodified samples (84.46 +/- 3.86). In vitro cell culture was carried out by L929 mouse fibroblast cell line in order to examine the effects of plasma surface modification on cell material interactions. Standard MIT assay showed improved cell viability on/within modified scaffolds confirmed with the observations of the cell attachment and the morphology by means of SEM, fluorescence and confocal imaging. The experiments performed in the study proved the enhanced biocompatibility of the nozzle type dry air plasma modified scaffolds. (C) 2016, The Society for Biotechnology, Japan. All rights reserved. en_US
dc.description.sponsorship Scientific and Technological Research Council of Turkey (TUBITAK) [112M043] en_US
dc.description.sponsorship The authors would like to thank The Scientific and Technological Research Council of Turkey (TUBITAK) for the scientific and financial support (Grant No. 112M043) and the authors would also like to acknowledge the priceless contribution of Academic Writing Advisory Center (AWAC) of Atilim University to this study in linguistic terms. en_US
dc.identifier.citationcount 18
dc.identifier.doi 10.1016/j.jbiosc.2016.01.004
dc.identifier.endpage 239 en_US
dc.identifier.issn 1389-1723
dc.identifier.issn 1347-4421
dc.identifier.issue 2 en_US
dc.identifier.pmid 26906227
dc.identifier.scopus 2-s2.0-84975318489
dc.identifier.scopusquality Q2
dc.identifier.startpage 232 en_US
dc.identifier.uri https://doi.org/10.1016/j.jbiosc.2016.01.004
dc.identifier.uri https://hdl.handle.net/20.500.14411/642
dc.identifier.volume 122 en_US
dc.identifier.wos WOS:000381835800016
dc.identifier.wosquality Q3
dc.institutionauthor Özkan, Ozan
dc.institutionauthor Şaşmazel, Hilal Türkoğlu
dc.language.iso en en_US
dc.publisher Soc Bioscience Bioengineering Japan en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.scopus.citedbyCount 19
dc.subject Nozzle type atmospheric plasma en_US
dc.subject Dry air en_US
dc.subject Electrospun poly (epsilon-caprolactone) en_US
dc.subject Electrospun chitosan en_US
dc.subject L929 fibroblast cells en_US
dc.title Effects of Nozzle Type Atmospheric Dry Air Plasma on L929 Fibroblast Cells Hybrid Poly (ε-caprolactone)/Chitosan (ε-Caprolactone) Scaffolds Interactions en_US
dc.type Article en_US
dc.wos.citedbyCount 18
dspace.entity.type Publication
relation.isAuthorOfPublication 741bfc12-0d53-48d4-bfa2-08290dbfcbeb
relation.isAuthorOfPublication 89a1446a-af3c-4bd3-a3f6-5f29625b68fd
relation.isAuthorOfPublication.latestForDiscovery 741bfc12-0d53-48d4-bfa2-08290dbfcbeb
relation.isOrgUnitOfPublication 7cf7435b-3e8e-404e-adee-0f6f7dc8e070
relation.isOrgUnitOfPublication.latestForDiscovery 7cf7435b-3e8e-404e-adee-0f6f7dc8e070

Files

Collections