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Permanent URI for this collectionhttps://hdl.handle.net/20.500.14411/18
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Article Citation - WoS: 2Citation - Scopus: 2The Relationship Between Lung Function, Exercise Capacity, Oxidant and Antioxidant Response in Primary Ciliary Dyskinesia and Cystic Fibrosis(Turkish J Pediatrics, 2024) Kartal, Yasemin; Ozel, Cemile Bozdemir; Cakmak, Aslihan; Ulu, Hazal Sonbahar; Ince, Deniz Inal; Tural, Dilber Ademhan; Sabuncuoglu, Suna; Özçelik, Uğur; Inal-ınce, Deniz; Budak, Murat; Arıkan, HülyaBackground. There is a need to identify the complex interplay between various physiological mechanisms in primary ciliary dyskinesia (PCD) and cystic fibrosis (CF). The study investigated the interaction between respiratory function, exercise capacity, muscle strength, and inflammatory and oxidant/antioxidant responses in patients with PCD and CF. Methods. The study included 30 PCD patients, 30 CF patients, and 29 age and sex-matched healthy subjects. Exercise capacity was assessed using the modified shuttle walk test (MSWT). Handgrip strength (HGS) was used to evaluate general muscle strength. Oxidative stress-inflammatory parameters were also assessed. Pulmonary function test was performed by spirometry. Regarding the forced expiratory volume in 1 second (FEV1) 1 ) z-score, patients with PCD and CF were subdivided into normal, mild, and severe/moderate groups. Results. Forced vital capacity (FVC) z-scores were lower in PCD and CF patients than controls. FEV1, 1 , FEV1/ 1 / FVC, peak expiratory flow (PEF), and forced mid expiratory flow (FEF 25-75% ) z-scores were lower in PCD than in the other groups. HGS was lower in both mild PCD and normal CF patients relative to the controls. MSWT distance was lower in severe/moderate PCD patients than controls. Catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), and malondialdehyde (MDA) levels did not differ significantly among the study groups, but superoxide dismutase (SOD) level in severe/moderate PCD, and glutathione (GSH) level in normal CF were higher than in controls. Interleukin-6 (IL-6) level was higher in patients with normal PCD and CF compared to the controls. IL-1 beta level was higher in PCD compared to controls. Additionally, correlations among these parameters were also determined in some patient groups. Conclusion. Homeostasis related to respiratory function, aerobic performance, muscle strength, inflammatory response, and oxidant/antioxidant balance were affected in PCD and CF. Evaluating these mechanisms together may contribute to elucidating the pathophysiology of these rare diseases.Article Citation - WoS: 4Citation - Scopus: 3Management of Chronic Hepatitis B in Special Patient Groups: a Consensus Report of the Study Group for Viral Hepatitis of the Turkish Society of Clinical Microbiology and Infectious Diseases-2023(Doc design informatics Co Ltd, 2023) Tekin, Sueda; Karakecili, Faruk; Binay, Umut Devrim; Celik, Ilhami; Tulek, Necla; Tutuncue, Ediz; Demirturk, Nese; Tütüncü, Ediz; Yılmaz, EmelTurkish Society of Clinical Microbiology and Infectious Diseases Viral Hepatitis Working Group updated the consensus report on the management of chronic hepatitis B (CHB) in private hosts and special situations. Relevant literature and international guidelines are reviewed, and agreed recommendations are presented at the end of each chapter. In the report, treatment of hepatitis B virus (HBV) and human immunodeficiency virus (HIV) coinfection, management of HBV and hepatitis C virus (HCV) coinfection, management of CHB in severe COVID-19 patients, non-transplant immunocompromised patients.Article Gold-Assembled Silica-Coated Cobalt Nanoparticles as Efficient Magnetic Separation Units and Surface-Enhanced Raman Scattering Substrate Lütfiye Sezen Yildirim1,, Murat Kaya2,∗,, Mürvet Volkan(Tubitak Scientific & Technological Research Council Turkey, 2019) Yıldırım, Lütfiye Sezen; Kaya, Murat; Volkan, MürvetMagnetic and optical bifunctional nanoparticles that combine easy separation, preconcentration, and efficientSERS capabilities have been fabricated with high sensitivity and reproducibility through a low-cost method. Thesegold nanoparticles attached on magnetic silica-coated cobalt nanospheres (Co@SiO2 /AuNPs) display the advantageof strong resonance absorption due to gaps at nanoscale between neighboring metal nanoparticles bringing large fieldenhancements, known as “hot spots”. The prepared particles can be controlled by using an external magnetic field,which makes them very promising candidates in biological applications and Raman spectroscopic analysis of dissolvedorganic species. The magnetic property of the prepared particles lowers the detection limits through preconcentrationwith solid-phase extraction in SERS analysis. The performance of the prepared nanostructures was evaluated as a SERSsubstrate using brilliant cresyl blue (BCB) and rhodamine 6G (R6G) as model compounds. The solid-phase affinityextraction of 4-mercapto benzoic acid (4-MBA) using bifunctional Co@SiO2 /AuNPs nanoparticles followed by magneticseparation and the measurement of the SERS signal on the same magnetic particles without elution were investigated.Approximately 50-fold increase in SERS intensity was achieved through solid-phase extraction of 8.3 × 10 −6 M 4-MBAin 10 min.Review Hepatit B İnfeksiyonlarının İmmünopatogenezi(Bilimsel Tip Yayinevi, 2019) Tülek, NeclaHepatit B infeksiyonları tüm dünyada majör sağlık problemleri arasında olup, siroz ve hepatoselüler karsinomanın en önemli nedenidir.Tedavide gelişmelere rağmen henüz kronik hepatit B infeksiyonunda kür sağlanamamaktadır. Hepatit B infeksiyonlarında klinik sonuçinfeksiyonun alınma yaşı, viral yük ve konağın immün yanıtına bağlıdır. Akut hepatitlerde klinik seyir subklinik hastalıktan, anikterikhepatite, ikterik hepatite ve fulminant hepatite kadar değişebilirken, kronik hepatitlerde hastalığın seyri asemptomatik inaktif durumdankronik hepatite, siroz ve hepatoselüler karsinomaya kadar değişebilir. Hepatit B virüsü hepatositlerde sitopatik olmayarak çoğalır veklinik sendromların çoğu immün yanıt ile ilişkilidir. İmmün sistem aktivasyonu virüsün eliminasyonunu sağladığı gibi karaciğer hasarınada neden olur. Hepatit B patogenezinde hücresel immün yanıt özellikle de CD8+ T hücreler santral olmasına rağmen immün sistemindiğer komponentleri de katkıda bulunur. Kronik hepatitli hastalarda hem doğal hem de adaptif immün yanıt zayıflamış ve T hücreyanıtı tükenmiş durumdadır. İmmünopatolojideki mekanizmaların daha iyi anlaşılması, yeni etkin tedavi stratejilerinin geliştirilmesindeyararlı olacaktır.Article Citation - WoS: 3Citation - Scopus: 4Identification of Bacterial Diversity of Bee Collected Pollen and Bee Bread Microbiota by Metagenomic Analysis(Aves, 2022) Arserim Ucar, Dilhun Keriman; Yurt, Mediha Nur Zafer; Tasbasi, Behiye Busra; Acar, Elif Esma; Yegin, Zeynep; Ozalp, Veli Cengiz; Sudagidan, Mert; Uçar, Dilhun Keriman Arserim; Ozalp, Cengiz; Arserim-uçar, Dılhun KerimanThis study investigated the bacterial diversities of bee-collected pollen and bee bread of Apis mellifera in Turkey. The bacterial community structure of 14 bee pollen from Bingol, Konya, and Hakkari and 11 bee bread samples from Bingol were studied using 16 S rRNA amplicon sequencing and metagenomic analysis. The dominant bacterial phylum in pollen and bee bread samples was Firmicutes, followed by Proteobacteria. In pollen and bee bread samples, Bacillaceae, Clostridiaceae, Enterococcaceae, and Enterobacteriaceae were identified as dominant bacterial families. At the genus level, Bacillus, Clostridium sensu stricto, and Enterococcus were dominant bacteria in both pollen and bee bread samples. The most abundant species was Clostridium perfringens in both pollen and bee bread samples. Escherichia vulneris, Enterococcus faecalis, Bacillus cereus, Enterococcus casseliflavus, and Cronobacter malonaticus were identified with high reads in pollen samples. In bee bread samples, E. faecalis, Clostridium bifermentans, and Pantoea calida were abundant bacterial species. Alpha diversity showed that pol-3 sample had the highest diversity. Beta-diversity plots separated the pollen samples into four main groups and bee bread samples into three main groups. Our results indicated that the culture-independent metagenomic analysis will be a valuable tool for determining the microbial diversity of bee products produced in Bingol-Turkey one of the important centers of apiculture.Article Identification of Bacterial Vaginal Microbiota Via Metagenomic Approach(Galenos Publ House, 2022) Ucak, Samet; Sudagidan, Mert; Yurt, Mediha Nur Zafer; Tasbasi, Behiye Busra; Acar, Elif Esma; Tuna, Bilge Guvenc; Ozalp, Veli Cengiz; Ozalp, Cengiz; Dogan, SonerAim: The aim of the current study was to identify vaginal bacterial microbiota of 38 Turkish women using the high -throughput next -generation sequencing and metagenomic approach at different taxonomic levels from the kingdom to the species level. Materials and Methods: Vaginal swab samples (n=38) were collected in the DNA/RNA shield collection tubes at Yeditepe University Hospital, Department of Obstetrics and Gynecology in June 2021 and DNA extraction was performed by ZymoBIOMICS DNA miniprep kit. The information related to age, marital status, preliminary diagnosis and anamnesis status of patients were collected. To determine the vaginal microbiota, a metagenomic approach was applied using 16S rRNA amplicon sequencing. Results: The dominant phylum Firmicutes was followed by Proteobacteria, Actinobacteria, Tenericutes, Fusobacteria, and Synergistetes in the vaginal samples. Lactobacillus was the most abundant genus followed by Prevotella, Enterobacter, Gardnerella, and Dialister. Lactobacillus iners was dominant at the species level in vaginal swab samples, followed by Gardnerella vaginalis, Enterobacter tabaci, Prevotella timonensis, Prevotella bivia, and Lactobacillus jensenii. Canonical correspondence analysis (CCA) showed that Proteobacteria and Fusobacteria were mainly related to married/single variable with the highest percentages, whereas Actinobacteria and Tenericutes were related to age variable at the phylum level. Campylobacter , Atopobium , Enterobacter , and Lactococcus were mainly found in married/single variable with the highest percentages, whereas Anaerococcus, Streptococcus, Sutterella , and Veillonella were related to age. Moreover, CCA showed that Campylobacter ureolyticus, Lb. jensenii , and Atopobium vaginae were associated with married/single variable, whereas Lactobacillus johnsonii and G. vaginalis were found in age variable with the highest percentages at the species level. Conclusion: Vaginal diseases are still a major public health concern. The vaginal microbiota, which has been studied in more depth in recent years, has been discovered to be more complicated than previously imagined thanks to technological developments. More patient investigations are needed to confirm and develop these findings.Article Citation - Scopus: 1Determination of the Knowledge Levels of Nurses Regarding Central Venous Catheter Care(Galenos Publ House, 2022) Turkkan, Hicran; Ayyildiz, Tulay Kuzlu; Sonmez, MunevverIntroduction: It is important that pediatric nurses have sufficient knowledge in the prevention of central catheter-related blood circulation infections. This study was carried out to determine knowledge levels of the nurses, who were working in pediatric clinics, regarding central venous catheter care.Methods: The universe of the study was composed of 118 nurses, who were working in pediatric clinic of a public hospital (n=50) and a training hospital (n=68) between May-June 2019. No sample was selected; and 88 nurses, who were eligible for the sample and study criteria and approved to participate during the dates of data collection (May-June 2019) were included in the study. 75% of the universe was reached. Personal information form for the identification of demographic characteristics and working lives of the nurses and the form for the knowledge levels of the nurses regarding central venous catheter care were used to collect data.Results: At the end of statistical assessment, central venous catheter care mean score of nurses was found to be 8.35+2.36 (0-12). In addition, it was determined that no significant differences were found between central venous catheter scores of the nurses based on age, sex, marital status, working institution, working department, working year and their states of providing central venous catheter care (p>0.05). A significant difference was detected between nurses' states of having knowledge and education regarding central venous catheter and their central venous catheter scores (p<0.05). Conclusion: It was found that the knowledge points of the nurses for central venous catheter care were insufficient. It is recommended to increase in-service training in line with the protocol/procedure/ instructions regarding central venous catheter care.Article Citation - WoS: 2The Impact of Vaccination Among Hospitalized Patients With the Diagnosis of Covid-19(Doc design informatics Co Ltd, 2023) Yıldız, Yeşim; Özger, Hasan Selçuk; Acar, Ali; Keskin, Ayşegül Seremet; Binay, Umut Devrim; Ünlü, Gülten; Diseases, The Adult Immunization Study Group Of The Turkish Society Of Clinical Microbiology And Infectious; Seremet-Keskin, Aysegul; Azap, Alpay; Senol, Esin; Bayındır, Yasar; Kaya, Safak; Saygideger, Yasemin; Mete, Ayşe ÖzlemObjective: We aimed to investigate the vaccination status and the risk factors for the in- tensive care unit (ICU) support need of the laboratory-confirmed breakthrough COVID-19 infection inpatients. Materials and Methods: This multi-center point-prevalence study was conducted on inpa- tients, divided into two groups as ‘fully’ and ‘partially’ vaccinated according to COVID-19 vaccination status. Results: Totally 516 patients were included in the study. The median age was 65 (55-77), and 53.5% (n=276) of the patients were male. Hypertension (41.9%, n=216), diabetes mel- litus (DM) (31.8%, n=164), and coronary artery disease (CAD) (16.3%, n=84) were the pre- dominant comorbidities. Patients were divided into two groups ICU (n=196) and non-ICU (n=301). Hypertension (p=0.026), DM (p=0.048), and congestive heart failure (CHF) (p=0.005) were significantly higher in ICU patients and the median age was younger among non-ICU patients (p=0.033). Of patients, 16.9% (n=87) were fully vaccinated, and this group’s need for ICU support was statistically significantly lower (p=0.021). Conclusion: We conclude that older age, hypertension, DM, CHF, and being partially vacci- nated were associated with the need for ICU support. Therefore, all countries should con- tinuously monitor post-vaccination breakthrough COVID-19 infections to determine the national booster vaccine administration approach that will provide vulnerable individuals the highest protection.Article Risk Factors for Rectal Colonization with Carbapenemase Producing <i>Enterobacteriaceae</i>(Bilimsel Tip Yayinevi, 2021) Hekimoglu, Sirin; Ertem, Gunay; Yucel, Mihriban; Basar, Hulya; Kaya Kilic, Esra; Ozcan, Ayse; Tulek, Necla; Kılıç, Esra Kaya; Erdınc, Fatma SebnemIntroduction: Carbapenem-resistant Enterobacteriaceae (CRE) is the most common pathogens acquired in intensive care units (ICU). Materials and Methods: This study was designed as prospective observational study at the Ankara Healthcare Application and Research Center of the University of Health Sciences. The study was conducted between September 2017 and May 2018 in the tertiary Anesthesiology and Reanimation ICU. Rectal swab samples were taken from each hospitalized patient during hospitalization and weekly thereafter. Sixty-five patients with CRE colonization were considered as the study group and 65 patients without CRE colonization were considered as the control group. Results: The 51% of the cases are male and 49% are females. The mean age of all cases was 69.1 +/- 17.6 and the median age was 73. The majority of bacteria grown in rectal swabs are constituted by Klebsiella pneumoniae (43-58.1%) and Escherichia colt (16-21.6%). Duration of hospitalization in the ICU was 19.6 +/- 15.8 in the control group and 48.5 +/- 38.2 in the study group. Duration of hospitalization in CRE-infected patients were longer than non-infected patients (p<0.001). The use of a central venous catheter and tracheostomy showed statistically significant difference between the groups (p< 0.05). Nineteen cases (29.2%) had CRE positivity at their admission to the ICU. Eighteen of these patients came from another clinical service or hospital (p< 0.005). In the control group, carbapenem was used in a mean of 11.9 +/- 9.9 days and in the study group a mean of 16.3 +/- 8.5 days (p= 0.002). In multivariate analysis of risk factors, duration of hospitalization in the ICU increases the risk of CRE positivity in rectal culture 1.04 (95%GA, 1,005-1,072, p= 0.024) times more. Conclusion: In our study, it can be said that it is important to question the indications of invasive procedures to be applied to the patients. We recommend that patients should be admitted to service follow-up when the ICU follow-up and treatment indications are terminated. Carbapenem treatment times should be shortened. As a result, it would be wise to take rectal surveillance cultures in ICUs to detect and control carbapenem-resistant strains.Article Evaluation of Covid-19 Cases Who Received Immunosuppressive Therapy at a Tertiary Care Hospital(Doc design informatics Co Ltd, 2022) Sayar, Merve Sefa; Acar, Ali; Bulut, Dilek; Celik, Sebahattin; Oguz, EminObjective: Immunosuppressive drugs are included in the treatment protocols of severe COVID-19 cases that may present with cytokine storm. In this study, we aimed to examine the characteristics of COVID-19 patients who received immunosuppressive therapy Methods: Patients diagnosed with COVID-19 and followed in the quarantine wards of our hospital between 01.03.2020-24.05.2020 were included in the study. Patient information was obtained retrospectively from patient files and discharge reports, and 263 COVID-19 patients-aged 18 and over-were included in the study. Results: The median age of the patients who received immunosuppressive therapy was 60.74 +/- 1.96, and 39.6% of them were women. The average symptom duration of the patients examined in the study at the time of admission to the hospital was 4.59 +/- 0.29 days. There was a significant difference in age, lymphocyte count, neutrophil/lymphocyte ratio, C-reactive protein, ferritin, D-Dimer, blood oxygen saturation, fever after antiviral therapy, qSOFA score, and total hospital stay between the two groups that received and did not receive immunosuppressive therapy (p<0.05). The need for immunosuppressive therapy increased 15.9 times over the age of 40, 15.6 times in the presence of diffuse involvement on thoracic CT, 6.6 times in the presence of chronic disease, 2.7 times in the presence of thrombocytopenia, and 1.7 times in the presence of lymphopenia (p <0.05).Conclusions: We observed that patients whose immunosuppressive therapy was added to their treatment protocols were admitted to the hospital later than others, had more prevalent involvement in thorax CT, high acute phase reactants, low SPO2, and more than one underlying disease.