Browsing by Author "Ulag, Songul"
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Article Citation Count: 0Advanced 3D printed bone scaffolds with sodium alginate/Tri-calcium phosphate/probiotic bacterial hydroxyapatite: Enhanced mechanical and biocompatible properties for bone tissue engineering(Elsevier Sci Ltd, 2024) Nouri, Sabereh; Şaşmazel, Hilal Türkoğlu; Emtiazi, Giti; Ulag, Songul; Gunduz, Oguzhan; Koyuncu, Ayse Ceren Calikoglu; Roghanian, Rasoul; Sasmazel, Hilal Turkoglu; Metallurgical and Materials EngineeringIntroduction: The increasing prevalence of severe bone diseases, such as osteoporosis and critical bone defects, necessitates the development of more effective bone substitutes. This study addresses this need by investigating 3D-printed bone scaffolds composed of sodium alginate and tricalcium phosphate, enhanced with three distinct types of hydroxyapatite (HA): bovine-derived HA, commercially available HA, and HA enriched with probiotic bacteria. We aim to evaluate the performance of these scaffolds in terms of mechanical strength, biocompatibility, and their ability to support bone regeneration. Methods: The scaffolds were analyzed through various tests, including X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC) to characterization. Scanning Electron Microscopy (SEM) was used to examine pore structure, while swelling and degradation tests evaluated the scaffold's stability. Compression testing determined mechanical strength, and in vitro cell culture assays assessed cell proliferation, osteogenic differentiation, and biomineralization. Results: SEM results indicated that 3D scaffolds with probiotic bacterial HA had the desired 472 mu m pore size. These scaffolds demonstrated a strain of 29.26 % and a compressive strength of 10 MPa, meeting the mechanical standards of human trabecular bone. Cell culture studies revealed enhanced cell proliferation by 50 %, osteogenic differentiation with 15.3 U/mg ALP activity, and 1.22-fold biomineralization, suggesting they are highly biocompatible and promote bone growth. Conclusion: Probiotic bacterial HA scaffolds exhibit ideal properties and biocompatibility, enhancing bone regeneration and serving as an ideal alternative to chemical types.Editorial Citation Count: 0Editorial: Biofabricated materials for tissue engineering(Frontiers Media Sa, 2024) Şaşmazel, Hilal Türkoğlu; Gunduz, Oguzhan; Ramalingam, Murugan; Ulag, Songul; Metallurgical and Materials Engineering[No Abstract Available]Article Citation Count: 4Fucoidan-loaded electrospun Polyvinyl-alcohol/Chitosan nanofibers with enhanced antibacterial activity for skin tissue engineering(Elsevier, 2023) Puigmal, Arnau Cuesta; Ayran, Musa; Ulag, Songul; Altan, Eray; Guncu, Mehmet Mucahit; Aksu, Burak; Gunduz, OguzhanThe polymeric nanofiber may interact and control certain regeneration processes at the molecular level to repair damaged tissues. This research focuses on the development of characterization and antibacterial capabilities of polyvinyl alcohol (PVA)/chitosan (CS) nanofibres containing fucoidan (FUC) for tissue engineering as a skin tissue substitute. A control group consisting of 13% PVA/(0.1)% CS nanofiber was prepared. To confer anti-bacterial properties to the nanofiber, 10, 20, and 30 mg of FUC were incorporated into this control group. The scanning electron microscope (SEM) proved the homogeneous and beadless structures of the nanofibers. The antibacterial activity of the 13% PVA/(0.1)% CS/(10, 20, 30) FUC was tested against the S.aureus and E.coli and the results showed that with FUC addition, the antibacterial activities of the nanofibers increased. The biocompatibility test was performed with a fibroblast cell line for 1, 3, and 7 days of incubation and the results demonstrated that FUC addition enhanced the bioactivity of the 13% PVA/(0.1)% CS nanofibers. In addition, the biocompatibility results showed that 13% PVA/(0.1)% CS/10 FUC had the highest viability value for all incubation periods compared to the others. In addition, the tensile test results showed that; the maximum tensile strength value was observed for 13% PVA/(0.1)% CS/10 FUC nanofibers.