Browsing by Author "Topsakal, Aysenur"

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Citation - WoS: 24
Citation - Scopus: 28
Development of Amoxicillin-Loaded Electrospun Polyurethane/Chitosan Β-Tricalcium Phosphate Scaffold for Bone Tissue Regeneration
(Ieee-inst Electrical Electronics Engineers inc, 2018) Topsakal, Aysenur; Uzun, Muhammet; Ugar, Gaye; Ozcan, Aslihan; Altun, Esra; Oktar, Faik Nuzhet; Gunduz, Oguzhan
Biocompatible nanocomposite electrospun fibers containing Polyurethane/Chitosan/beta-Tri calcium phosphate with diverse concentrations were designed and produced through the electrospinning process for bone tissue engineering applications. After the production process, density measurement, viscosity, electrical conductivity, and tensile strength measurement tests were carried out as physical analyses of blended solutions. The chemical structural characterization was scrutinized using Fourier transform infrared spectrometer (FTIR), and scanning electron microscopy (SEM) was used to observe the morphological details of developed electrospun scaffolds. Cell viability, attachment, and proliferation were performed using a L929 fibroblast cell line. Based on the physical, SEM, FTIR analysis, and cell culture studies, preferable nanofiber composition was selected for further studies. Amoxicillin (AMX) was loaded to that selected nanofiber composition for examination of the drug release. In comparison with other studies on similar AMX controlled products, higher drug loading and encapsulation efficiencies were obtained. It has been clearly found that the developed nanofiber composites have potential for bone tissue engineering applications.
Citation - WoS: 35
Citation - Scopus: 44
Study on the Cytocompatibility, Mechanical and Antimicrobial Properties of 3d Printed Composite Scaffolds Based on Pva/ Gold Nanoparticles (aunp)/ Ampicillin (amp) for Bone Tissue Engineering
(Elsevier, 2021) Topsakal, Aysenur; Midha, Swati; Yuca, Esra; Tukay, Ari; Sasmazel, Hilal Turkoglu; Kalaskar, Deepak M.; Gunduz, Oguzhan
Over the years, gold nanoparticles (AuNP) have been widely used in several biomedical applications related to the diagnosis, drug delivery, bio-imaging, photo-thermal therapy and regenerative medicine, owing to their unique features such as surface plasmon resonance, fluorescence and easy surface functionality. Recent studies showed that gold nanoparticles display positive effect on osteogenic differentiation. In line with this effect, 3-Dimesional (3D) scaffolds that can be used in bone tissue were produced by exploiting the properties of gold nanoparticles that increase biocompatibility and support bone tissue development. In addition, ampicillin was added to the scaffolds containing gold nanoparticles as a model drug to improve its antimicrobial properties. The scaffolds were produced as composites of polyvinyl alcohol (PVA) main matrix as PVA, PVA/AuNP, PVA/Ampicillin (AMP) and PVA/AuNP/AMP. Scanning Electron Microscopy (SEM) Fourier Transform Infrared Spectroscopy (FTIR), tensile measurement tests, and in vitro applications of 3D scaffolds were performed. As depicted by SEM, scaffolds were produced at pore sizes appropriate for bone tissue regeneration. According to FTIR results, there was no modification observed in the AMP, PVA and gold nanoparticles due to mixing in the resultant scaffolds. In vitro results show that 3D printed composite scaffold based on PVA/AuNP/AMP are biocompatible, osteo-inductive and exhibit antimicrobial properties, compared to PVA scaffolds. This study has implications for addressing infections during orthopedic surgeries. The PVA-based gold nanoparticle 3D tissue scaffold study containing ampicillin covers a new study compared to other articles based on gold nanoparticles.
Citation - WoS: 16
Citation - Scopus: 17
Synthesis and Characterization of Antibacterial Drug Loaded Β-Tricalcium Phosphate Powders for Bone Engineering Applications
(Springer, 2020) Topsakal, Aysenur; Ekren, Nazmi; Kilic, Osman; Oktar, Faik N.; Mahirogullari, Mahir; Ozkan, Ozan; Gunduz, Oguzhan
Powders of beta-tricalcium phosphate [beta-TCP, beta-Ca-3(PO4)(2)] and composite powders of beta-TCP and polyvinyl alcohol (PVA) were synthesized by using wet precipitation methods. First, the conditions for the preparation of single phase beta-TCP have been delineated. In the co-precipitation procedure, calcium nitrate and diammonium hydrogen phosphate were used as calcium and phosphorous precursors, respectively. The pH of the system was varied in the range 7-11 by adding designed amounts of ammonia solution. The filtered cakes were desiccated at 80 degrees C and subsequently calcined at different temperatures in the range between 700-1100 degrees C. Later on, rifampicin form II was used to produce drug-loaded beta-TCP and PVA/beta-TCP powders. All the synthesized materials have been characterized from morphological (by scanning electron microscopy) and structural-chemical (by X-ray diffraction and Fourier transform infrared spectroscopy) point of view. The drug loading capacity of the selected pure beta-TCP powder has been assessed. The biological performance (cytocompatibility in fibroblast cell culture and antibacterial efficacy against Escherichia coli and Staphylococcus aureus) has been tested with promising results. Application perspectives of the designed drug-bioceramic-polymer blends are advanced and discussed. [GRAPHICS] .