Browsing by Author "Onur, Mehmet Ali"

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Citation - WoS: 23
Citation - Scopus: 25
Comparison of Cellular Proliferation on Dense and Porous Pcl Scaffolds
(Ios Press, 2008) Sasmazel, Hilal Tuerkoglu; Gumusderelioglu, Menemse; Gurpinar, Aylin; Onur, Mehmet Ali; Metallurgical and Materials Engineering
In this contribution, PCL (poly-e caprolactone) scaffolds were prepared by solvent-casting/particle-leaching technique in the presence of two pore formers, PEG(4000) or sucrose molecules in different quantities (0, 10, 20, 30, 40, 50, 55 w/w% PEG(4000)/PCL; 10, 20 w/w% Sucrose/ PCL). The surface and bulk properties of the resulting scaffolds were studied by SEM, DSC and FTIR. SEM photographs showed that, macroporosity was obtained in the PCL structures prepared with sucrose crystals while microporous structure was obtained in the presence of PEG(4000) molecules. Average pore diameters calculated from SEM photographs were 40.1 and 191.2 mu m for 40% PEG(4000)/PCL and 10% Sucrose/PCL scaffolds, respectively. The DSC and FTIR results confirmed that there is no any interaction between pore formers and PCL during structural formation, and both pore formers, PEG(4000) and sucrose, remained independently in the scaffolds. L929 mouse fibroblast cells were seeded onto PCL structures and maintained during 7 days to evaluate cell proliferation. Cell culture results showed that, 10% Sucrose/ PCL scaffold was the most promising substrate for L929 cell growth due to 3-D architecture and macroporous structure of the scaffold.
Citation - WoS: 13
Citation - Scopus: 13
In Vitro and in Vivo Bacterial Antifouling Properties of Phosphite of Plasma-Treated Silicone
(Ice Publishing, 2019) Akdogan, Ebru; Demirbilek, Murat; Sen, Yasin; Onur, Mehmet Ali; Azap, Ozlem Kurt; Sonmez, Erkin; Mutlu, Mehmet
In order to improve their bacterial antifouling property, silicone surfaces were functionalized through the plasma polymerization (PP) technique using diethyl phosphite as the precursor. The functionalized surfaces were characterized using contact angle measurements, contact angle titration, Fourier transform infrared-attenuated total reflection spectroscopy and in vitro cytotoxicity assay. The amount of non-specific protein adsorption and the conformational changes of surface-adsorbed proteins were investigated. Antifouling properties of the surfaces were evaluated in vitro and in vivo. PP functionalization generated a hydrophilic and amphoteric surface with a very good protein and bacterial antifouling property and caused less conformational changes on the secondary structure of surface-adsorbed proteins. In in vivo conditions, no slime layer was formed around bacteria that adhered on the PPfunctionalized surface. It is concluded that the amphoteric nature of the PP-functionalized surface is the reason for the good antifouling property.