Browsing by Author "Gumusderelioglu, Menemse"
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Article Citation Count: 27Comparison of cellular proliferation on dense and porous PCL scaffolds(Ios Press, 2008) Şaşmazel, Hilal Türkoğlu; Gumusderelioglu, Menemse; Gurpinar, Aylin; Onur, Mehmet Ali; Metallurgical and Materials EngineeringIn this contribution, PCL (poly-e caprolactone) scaffolds were prepared by solvent-casting/particle-leaching technique in the presence of two pore formers, PEG(4000) or sucrose molecules in different quantities (0, 10, 20, 30, 40, 50, 55 w/w% PEG(4000)/PCL; 10, 20 w/w% Sucrose/ PCL). The surface and bulk properties of the resulting scaffolds were studied by SEM, DSC and FTIR. SEM photographs showed that, macroporosity was obtained in the PCL structures prepared with sucrose crystals while microporous structure was obtained in the presence of PEG(4000) molecules. Average pore diameters calculated from SEM photographs were 40.1 and 191.2 mu m for 40% PEG(4000)/PCL and 10% Sucrose/PCL scaffolds, respectively. The DSC and FTIR results confirmed that there is no any interaction between pore formers and PCL during structural formation, and both pore formers, PEG(4000) and sucrose, remained independently in the scaffolds. L929 mouse fibroblast cells were seeded onto PCL structures and maintained during 7 days to evaluate cell proliferation. Cell culture results showed that, 10% Sucrose/ PCL scaffold was the most promising substrate for L929 cell growth due to 3-D architecture and macroporous structure of the scaffold.Article Citation Count: 9Functionalization of Nonwoven Pet Fabrics by Water/O2 Plasma for Biomolecule Mediated Cell Cultivation(Wiley-v C H verlag Gmbh, 2010) Şaşmazel, Hilal Türkoğlu; Manolache, Sorin; Gumusderelioglu, Menemse; Metallurgical and Materials EngineeringThe main target of this study was to obtain COOH functionalities on the surface of 3D, nonwoven polyethylene terephthalate fabrics (NWPFs) by using low pressure water/O-2 plasma assisted treatment. The plasma treatments were performed in a cylindrical, capacitively coupled RF-plasma-reactor and then following steps were performed: in situ (oxalyl chloride vapors) gas/solid reaction to convert -OH functionalities into COCl groups; and hydrolysis under open laboratory conditions using air moisture for final-COOH functionalities. COOH and OH functionalities on the surfaces were detected quantitatively by fluorescent labeling techniques. The COOH-functionalized samples were biologically activated with insulin or heparin molecules by using spacer polyoxyethylene bis-amine (PEO). Successful immobilization was checked qualitatively using electron spectroscopy for chemical analysis (ESCA). The average amount of immobilized insulin and heparin onto NWPF surfaces were determined as 146.09 and 4.81 nmol.cm(-2), respectively. Our results showed that water/O-2 plasma assisted treatment worked very well for functionalization and biofunctionalization of 3D NWPF disks comparing with wet-chemistry methods. Cell culture experiments indicated that functionalization of NWPF disks and/or nanotopographies on the disk surfaces were effective on adhesion and proliferation of L929 mouse fibroblasts.Correction Citation Count: 0Influence of water/O2 plasma treatment on cellular responses of PCL and PET surfaces (vol 21, pg 123, 2011)(Ios Press, 2011) Şaşmazel, Hilal Türkoğlu; Aday, Sezin; Manolache, Sorin; Gumusderelioglu, Menemse; Metallurgical and Materials Engineering[No Abstract Available]Article Citation Count: 2Membrane supported poly(butylene adipate-co-terephthalate) nanofibrous matrices as cardiac patch: Effect of basement membrane for the fiber deposition and cellular behavior(Elsevier, 2022) Gültan, Tuğçe; Gumusderelioglu, Menemse; Chemical EngineeringElectrospun nanofibrous matrices are convenient biomaterials that mimic extracellular matrices for adhesion, migration, proliferation, and differentiation of cells. The aim of this study is to optimize the electrospinning parameters for poly(butylene adipate-co-terephthalate) (PBAT) nanofiber production by the response surface methodology (RSM) and to develop a suitable material design for the usability of PBAT in cardiac tissue engineering. Therefore, electrospun PBAT nanofibrous matrices collected on solvent-casted polycaprolactone (PCL) or PBAT basement membranes at optimized conditions. The attachment and proliferation behavior of the H9C2 rat cardiomyoblasts investigated on different PBAT and PCL surface features as a model cell line. For this purpose, neat PBAT and PCL films have been used comparatively with both random (R-PBAT) and aligned PBAT (A-PBAT) nanofibers coated films. The effect of polymer concentration, flow rate, applied voltage, and needle tip -connector distance on fiber diameter and alignment was examined in the electrospinning process and optimum processing parameters were determined by RSM. The PBAT nanofibers were spun on basement membranes with 10% (w/v) polymer concentration, 1 mL/h volumetric flow rate, 2000 rpm collector rotation velocity (for aligned ones), 15 kV applied voltage, and 20 cm needle tip-collector distance. The average diameter of random (R-PBAT) and aligned (A-PBAT) nanofibers was calculated as 555 +/- 126 nm and 417 +/- 137 nm. The mechanical test results showed that the alignment topography increased the elastic modulus of PBAT nanofibers compared to random matrices. The alignment of fibers found as 91% and 75% within the +/- 10? range for A-PBAT/SC-PBAT and A-PBAT/SC-PCL, respectively. These findings showed that usage of PCL, instead of PBAT, as basement membrane decrease the alignment of deposited nanofibers. A 7-day cell culture study conducted with H9C2 cells seeded samples to investigate the influence of these differences on cell behavior. The results indicated that the alignment of fibers provides a suitable topography to proliferate and spread in myocyte morphology for H9C2 cells especially compared to neat films. Cellular behavior and nanofiber deposition have been affected by the usage of various basement membrane polymers. These findings demonstrated that the usage of basement membrane as support material provides the required thickness and mechanical properties to the aligned PBAT nanofiber matrices, and this double layer structure might be a promising candidate for cardiac tissue engineering with further studiesArticle Citation Count: 9Synergistic effect of fabrication and stabilization methods on physicochemical and biological properties of chitosan scaffolds(Taylor & Francis As, 2021) Gültan, Tuğçe; Bektas Tercan, Seyma; Cetin Altindal, Damla; Gumusderelioglu, Menemse; Chemical EngineeringIn this study, the aim was to investigate the changes in the physical, chemical and biological properties of chitosan scaffolds obtained by freeze-drying and microwave-assisted gas foaming methods. Also, it was aimed to determine the most suitable one when scaffolds are subjected to different stabilization processes. To prevent the solubility of chitosan scaffolds, stabilization processes were carried out by treatment with ethanol (EtOH), sodium hydroxide (NaOH), or sodium bicarbonate (Na2CO3). The chemical and physical changes in the chitosan structure induced by different stabilization methods were investigated by characterization studies carried out comparatively with two-dimensional chitosan films. The results showed that, particularly, NaOH stabilization improved the physical, thermal, and mechanical properties of the bulk material. The wettability and surface roughness of the chitosan films were enhanced for cellular adhesion after stabilization. Cell culture studies revealed that variations in both fabrication and stabilization methods significantly affected in vitro cellular responses such as cell attachment, proliferation and viability. In conclusion, chitosan scaffolds fabricated by microwave-assisted gas foaming and stabilized by NaOH solution were found as the best structure due to their higher cellular activities.