A Combinatorial Approach to Regenerate the Periodontal Ligament and Cementum in a Nondental Microenvironment

dc.authorid Ramalingam, Murugan/0000-0001-6498-9792
dc.authorid Lee, Jung-Hwan/0000-0001-8678-5459
dc.authorid Kim, Hae-Won/0000-0001-6400-6100
dc.authorscopusid 37004374000
dc.authorscopusid 57223040583
dc.authorscopusid 57188589720
dc.authorscopusid 7103317859
dc.authorscopusid 36144545600
dc.authorscopusid 55783141000
dc.authorscopusid 56591525500
dc.authorwosid Ramalingam, Murugan/AAV-1702-2020
dc.contributor.author Guo, Yongwen
dc.contributor.author He, Mengting
dc.contributor.author Wang, Peiqi
dc.contributor.author Bai, Ding
dc.contributor.author Park, Jeong-Hui
dc.contributor.author Dashnyam, Khandmaa
dc.contributor.author Ramalingam, Murugan
dc.date.accessioned 2024-07-05T15:22:26Z
dc.date.available 2024-07-05T15:22:26Z
dc.date.issued 2023
dc.department Atılım University en_US
dc.department-temp [Guo, Yongwen; He, Mengting; Wang, Peiqi; Bai, Ding] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Dept Orthodont,State Key Lab Oral Dis, Chengdu 610041, Peoples R China; [Guo, Yongwen] Lanzhou Stomatol Hosp, Lanzhou 730031, Peoples R China; [Park, Jeong-Hui; Dashnyam, Khandmaa; Lee, Jung-Hwan; Kim, Hae-Won] Dankook Univ, Inst Tissue Regenerat Engn, Cheonan 31116, South Korea; [Park, Jeong-Hui; Lee, Jung-Hwan; Kim, Hae-Won] Dankook Univ, BK21 NBM Global Res Ctr Regenerat Med, Dept Nanobiomed Sci, Cheonan 31116, South Korea; [Park, Jeong-Hui; Lee, Jung-Hwan; Kim, Hae-Won] Dankook Univ, Mechanobiol Dent Med Res Ctr, Cheonan 31116, South Korea; [Huck, Olivier; Benkirane-Jessel, Nadia] Univ Strasbourg, INSERM UMR 1260, Regenerat Nanomed, F-67084 Strasbourg, France; [Ramalingam, Murugan] Basque Fdn Sci, IKERBASQUE, Bilbao 48013, Spain; [Ramalingam, Murugan] Univ Basque Country UPV EHU, Joined Venture TECNALIA & Sch Pharm, Joint Res Lab Adv Pharm Dev Initiat, Vitoria 01006, Spain; [Ramalingam, Murugan] Biomed Res Networking Ctr Bioengn Biomat & Nanomed, Carlos III Hlth Inst, Madrid 28029, Spain; [Ramalingam, Murugan] Bioaraba Hlth Res Inst, Jose Atxotegi S N, Vitoria 01009, Spain; [Ramalingam, Murugan] Chengdu Univ, Sch Basic Med Sci, Chengdu 610106, Peoples R China; [Ramalingam, Murugan] Binzhou Med Univ, Sch Basic Med Sci, Yantai 264003, Peoples R China; [Ramalingam, Murugan] Atilim Univ, Dept Met & Mat Engn, TR-06830 Ankara, Turkiye; [Ramalingam, Murugan] Furtwangen Univ, Inst Precis Med, D-78054 Villingen Schwenningen, Germany en_US
dc.description Ramalingam, Murugan/0000-0001-6498-9792; Lee, Jung-Hwan/0000-0001-8678-5459; Kim, Hae-Won/0000-0001-6400-6100 en_US
dc.description.abstract While treated dentin matrix (TDM) has been used for regeneration of dental tissues, the quality and quantity of regenerated periodontal tissue structure are suboptimal. The present study was undertaken to test whether the combined use of the TDM with dental follicle cells (DFCs) and Hertwig's epithelial root sheath (HERS) cells enhances the regeneration of periodontal structures in a nondental microenvironment. TDMs were fabricated from 3-month-old Sprague-Dawley (SD) rats. DFCs and HERS cells were isolated from postnatal 7-day SD rats. Purified DFCs and HERS cells, both in combination or alone, were seeded and cultured on TDM in vitro and characterized. The cell-seeded TDMs were subsequently implanted into a 3-month-old rat greater omentum for 6 weeks, and further histological evaluation was performed. The results showed that cells grew well on the surface of TDMs, and mineralized nodules could be seen, especially in the HERS + DFCs group. After transplantation in rat omentum, periodontal ligament-like fibers and cementum-like structures were observed around the TDM in 1/3 of the samples in both the HERS group and the DFCs group and in 2/3 of the samples in the HERS + DFCs group, while almost no attached tissue formation was found in the TDM only group. The formed cementum width and the periodontal ligament length were significantly larger in the HERS + DFCs group. The periodontal ligament-like fibers in the HERS + DFCs group were orderly arranged and attached to the cementum-like tissues, which resembled the cementum-periodontal structure. Therefore, the combined use of DFCs, TDM, and HERS cells may be a promising strategy for the regeneration of the periodontal structures, especially in the nondental microenvironment. en_US
dc.description.sponsorship National Natural Science Foundation of China [32171308]; IKERBASQUE Start-Up Grants; National Research Foundation of Korea [2018K1A4A3A01064257, 2021R1A5A2022318] en_US
dc.description.sponsorship This work was supported by the National Natural Science Foundation of China (No. 32171308), IKERBASQUE Start-Up Grants, and the National Research Foundation of Korea (2018K1A4A3A01064257 and 2021R1A5A2022318). en_US
dc.identifier.citationcount 0
dc.identifier.doi 10.1155/2023/1277760
dc.identifier.issn 1932-6254
dc.identifier.issn 1932-7005
dc.identifier.scopus 2-s2.0-85167708018
dc.identifier.scopusquality Q2
dc.identifier.uri https://doi.org/10.1155/2023/1277760
dc.identifier.uri https://hdl.handle.net/20.500.14411/2203
dc.identifier.volume 2023 en_US
dc.identifier.wos WOS:001042890300001
dc.identifier.wosquality Q2
dc.language.iso en en_US
dc.publisher Wiley en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/openAccess en_US
dc.scopus.citedbyCount 0
dc.subject [No Keyword Available] en_US
dc.title A Combinatorial Approach to Regenerate the Periodontal Ligament and Cementum in a Nondental Microenvironment en_US
dc.type Article en_US
dc.wos.citedbyCount 0
dspace.entity.type Publication

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