A Combinatorial Approach to Regenerate the Periodontal Ligament and Cementum in a Nondental Microenvironment
dc.authorid | Ramalingam, Murugan/0000-0001-6498-9792 | |
dc.authorid | Lee, Jung-Hwan/0000-0001-8678-5459 | |
dc.authorid | Kim, Hae-Won/0000-0001-6400-6100 | |
dc.authorscopusid | 37004374000 | |
dc.authorscopusid | 57223040583 | |
dc.authorscopusid | 57188589720 | |
dc.authorscopusid | 7103317859 | |
dc.authorscopusid | 36144545600 | |
dc.authorscopusid | 55783141000 | |
dc.authorscopusid | 56591525500 | |
dc.authorwosid | Ramalingam, Murugan/AAV-1702-2020 | |
dc.contributor.author | Guo, Yongwen | |
dc.contributor.author | He, Mengting | |
dc.contributor.author | Wang, Peiqi | |
dc.contributor.author | Bai, Ding | |
dc.contributor.author | Park, Jeong-Hui | |
dc.contributor.author | Dashnyam, Khandmaa | |
dc.contributor.author | Ramalingam, Murugan | |
dc.date.accessioned | 2024-07-05T15:22:26Z | |
dc.date.available | 2024-07-05T15:22:26Z | |
dc.date.issued | 2023 | |
dc.department | Atılım University | en_US |
dc.department-temp | [Guo, Yongwen; He, Mengting; Wang, Peiqi; Bai, Ding] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Dept Orthodont,State Key Lab Oral Dis, Chengdu 610041, Peoples R China; [Guo, Yongwen] Lanzhou Stomatol Hosp, Lanzhou 730031, Peoples R China; [Park, Jeong-Hui; Dashnyam, Khandmaa; Lee, Jung-Hwan; Kim, Hae-Won] Dankook Univ, Inst Tissue Regenerat Engn, Cheonan 31116, South Korea; [Park, Jeong-Hui; Lee, Jung-Hwan; Kim, Hae-Won] Dankook Univ, BK21 NBM Global Res Ctr Regenerat Med, Dept Nanobiomed Sci, Cheonan 31116, South Korea; [Park, Jeong-Hui; Lee, Jung-Hwan; Kim, Hae-Won] Dankook Univ, Mechanobiol Dent Med Res Ctr, Cheonan 31116, South Korea; [Huck, Olivier; Benkirane-Jessel, Nadia] Univ Strasbourg, INSERM UMR 1260, Regenerat Nanomed, F-67084 Strasbourg, France; [Ramalingam, Murugan] Basque Fdn Sci, IKERBASQUE, Bilbao 48013, Spain; [Ramalingam, Murugan] Univ Basque Country UPV EHU, Joined Venture TECNALIA & Sch Pharm, Joint Res Lab Adv Pharm Dev Initiat, Vitoria 01006, Spain; [Ramalingam, Murugan] Biomed Res Networking Ctr Bioengn Biomat & Nanomed, Carlos III Hlth Inst, Madrid 28029, Spain; [Ramalingam, Murugan] Bioaraba Hlth Res Inst, Jose Atxotegi S N, Vitoria 01009, Spain; [Ramalingam, Murugan] Chengdu Univ, Sch Basic Med Sci, Chengdu 610106, Peoples R China; [Ramalingam, Murugan] Binzhou Med Univ, Sch Basic Med Sci, Yantai 264003, Peoples R China; [Ramalingam, Murugan] Atilim Univ, Dept Met & Mat Engn, TR-06830 Ankara, Turkiye; [Ramalingam, Murugan] Furtwangen Univ, Inst Precis Med, D-78054 Villingen Schwenningen, Germany | en_US |
dc.description | Ramalingam, Murugan/0000-0001-6498-9792; Lee, Jung-Hwan/0000-0001-8678-5459; Kim, Hae-Won/0000-0001-6400-6100 | en_US |
dc.description.abstract | While treated dentin matrix (TDM) has been used for regeneration of dental tissues, the quality and quantity of regenerated periodontal tissue structure are suboptimal. The present study was undertaken to test whether the combined use of the TDM with dental follicle cells (DFCs) and Hertwig's epithelial root sheath (HERS) cells enhances the regeneration of periodontal structures in a nondental microenvironment. TDMs were fabricated from 3-month-old Sprague-Dawley (SD) rats. DFCs and HERS cells were isolated from postnatal 7-day SD rats. Purified DFCs and HERS cells, both in combination or alone, were seeded and cultured on TDM in vitro and characterized. The cell-seeded TDMs were subsequently implanted into a 3-month-old rat greater omentum for 6 weeks, and further histological evaluation was performed. The results showed that cells grew well on the surface of TDMs, and mineralized nodules could be seen, especially in the HERS + DFCs group. After transplantation in rat omentum, periodontal ligament-like fibers and cementum-like structures were observed around the TDM in 1/3 of the samples in both the HERS group and the DFCs group and in 2/3 of the samples in the HERS + DFCs group, while almost no attached tissue formation was found in the TDM only group. The formed cementum width and the periodontal ligament length were significantly larger in the HERS + DFCs group. The periodontal ligament-like fibers in the HERS + DFCs group were orderly arranged and attached to the cementum-like tissues, which resembled the cementum-periodontal structure. Therefore, the combined use of DFCs, TDM, and HERS cells may be a promising strategy for the regeneration of the periodontal structures, especially in the nondental microenvironment. | en_US |
dc.description.sponsorship | National Natural Science Foundation of China [32171308]; IKERBASQUE Start-Up Grants; National Research Foundation of Korea [2018K1A4A3A01064257, 2021R1A5A2022318] | en_US |
dc.description.sponsorship | This work was supported by the National Natural Science Foundation of China (No. 32171308), IKERBASQUE Start-Up Grants, and the National Research Foundation of Korea (2018K1A4A3A01064257 and 2021R1A5A2022318). | en_US |
dc.identifier.citation | 0 | |
dc.identifier.doi | 10.1155/2023/1277760 | |
dc.identifier.issn | 1932-6254 | |
dc.identifier.issn | 1932-7005 | |
dc.identifier.scopus | 2-s2.0-85167708018 | |
dc.identifier.scopusquality | Q2 | |
dc.identifier.uri | https://doi.org/10.1155/2023/1277760 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14411/2203 | |
dc.identifier.volume | 2023 | en_US |
dc.identifier.wos | WOS:001042890300001 | |
dc.identifier.wosquality | Q2 | |
dc.language.iso | en | en_US |
dc.publisher | Wiley | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | [No Keyword Available] | en_US |
dc.title | A Combinatorial Approach to Regenerate the Periodontal Ligament and Cementum in a Nondental Microenvironment | en_US |
dc.type | Article | en_US |
dspace.entity.type | Publication |