Dursun, Ali Doğan
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Name Variants
Dursun, Ali D. D.
D. Dursun
D.,Ali Dogan
Dursun A.
A., Dursun
Dursun, Ali
Ali Doan
Dursun, Ali Dogan
Dursun, A. D.
A.,Dursun
Dursun,A.D.
D., Ali Doğan
Dursun, Ali Doğan
D.,Ali Doğan
A.D.Dursun
Ali Doğan, Dursun
A. D. Dursun
Dursun, Ali D.
Ali Dogan, Dursun
D., Ali Dogan
D. Dursun
D.,Ali Dogan
Dursun A.
A., Dursun
Dursun, Ali
Ali Doan
Dursun, Ali Dogan
Dursun, A. D.
A.,Dursun
Dursun,A.D.
D., Ali Doğan
Dursun, Ali Doğan
D.,Ali Doğan
A.D.Dursun
Ali Doğan, Dursun
A. D. Dursun
Dursun, Ali D.
Ali Dogan, Dursun
D., Ali Dogan
Job Title
Doçent Doktor
Email Address
ali.dursun@atilim.edu.tr
ORCID ID
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID
Scholarly Output
35
Articles
32
Citation Count
113
Supervised Theses
0
35 results
Scholarly Output Search Results
Now showing 1 - 10 of 35
Article Citation Count: 9Irisin Protects Against Hind Limb Ischemia Reperfusion Injury(Dove Medical Press Ltd, 2021) Dursun, Ali Doğan; Polat, Yucel; Kilicarslan, Aydan; Sungu, Nuran; Kartal, Hakan; Dursun, Ali Dogan; Arslan, Mustafa; Basic SciencesAim: The aim of this study was to evaluate the effects of irisin in a murine model of hind limb ischemia reperfusion (I/R). Methods: The mice were divided into four groups (n = 6 in each group): control, irisin, ischemia reperfusion (I/R), and irisin-ischemia reperfusion (I-I/R). Irisin (0.5 mu g.g(-1), intraperitoneally [i.p.]) was administered 30 min before the I/R procedure. After 2 h of ischemia and 2.5 h of reperfusion, blood and tissue samples were taken for biochemical and histopathological analysis. The results were analyzed by Kruskal-Wallis and Mann-Whitney U-tests. Results: There was a statistically significant difference in the total antioxidant status (TAS) and total oxidant status (TOS) levels in all the groups. The TAS level in the I/R group was significantly lower than that in the control, irisin, and I-I/R groups, whereas the TOS level was significantly higher in the I/R group as compared with that in the other groups. Caspase3 activity and caspase-8 activity, indicators of inflammation, were significantly higher in the I/R and I-I/R groups as compared with those in the control and irisin groups. Conclusion: Irisin may have protective effects in skeletal muscle ischemia reperfusion injury.Article Citation Count: 0Effects of Pregabalin on Kidney Tissue in Spinal Cord Ischemia Reperfusion Injured Rats(Gazi Univ, Fac Med, 2021) Dursun, Ali Doğan; Inan, Nurten; Kucuk, Aysegul; Ozer, Abdullah; Dursun, Ali Dogan; Tosun, Murat; Arslan, Mustafa; Basic SciencesObjectives: The purpose of this study was to investigate the possible protective effects of low and high dose pregabalin that was administered in rat in a spinal cord ischemia-reperfusion (I/R) study model. Material and Method: We used 24 Wistar albino rats as subjects in our study. They were divided into 4 groups; randomized Control (C group), I/R (I/R group), I/R-low dose (30 mg/kg) pregabalin (I/R-LP group) and I/R-high dose (200 mg/kg) pregabalin (I/R-HP group). All groups have undergone a laparotomy intervention under anesthesia. In I/R group, a cross clamp was placed in the abdominal aorta just after the laparotomy for 120 minutes (to cause spinal cord ischemia injury) and then reperfusion was achieved by opening the vascular clamp. At the end of the study, kidney tissue was obtained for determining total oxidant status (TOS) and total antioxidant status (TAS) levels, histochemical and immunohistochemical determination. Results: Total Oxidative Status (TOS) enzyme activity was significantly higher in I/R group when compared to the control, I/R-LP and I/R-HP groups. Likewise, Total Antioxidant Status (TAS) enzyme activity was remarkably higher in I/R group when compared with the C, I/R-LP and I/R-HP groups. VEGF staining has yielded no expression in renal tissues. In microscopical analysis of the tissue slides which were immunohistochemically stained with p53 antibody, some crucial findings have been established as follows: As p53-expressing cells were not detected in the control group, the presence of p53-expressing cells were clearly identified at different intensities in several bowman capsules in the I/R group. However, no expression was detected in general tubules. Interestingly, p53 expression levels were prominently lower in low-dose pregabalin given group and considerably higher in the 200 mg/kg pregabalin administered group, which was more pronounced than the I/R group. Conclusion: Results established from the current study suggest that pregabalin given at different doses may have a partial protective effect in kidney tissues of rats undergone experimental spinal cord IR injury.Article Citation Count: 0Is increased activator protein 1 in cerebrospinal fluid as a potential biomarker that distinguishes idiopathic intracranial from sclerosis?(Aepress Sro, 2024) Dursun, Ali Doğan; Celik, Humeyra; Dursun, Ali Dogan; Ankarali, Handan; Turkoglu, Sule Aydin; Basic SciencesOBJECTIVES: To distinguish whether idiopathic intracranial hypertension (IIH) is a condition predisposing to multiple sclerosis (MS) or an isolated disease, the current gene transcription factor Activator Protein -1 (AP -1) was evaluated with its potential to differentiate both diseases. BACKGROUND: The aim of this study was to investigate the use of AP -1 as biomarkers for the discrimination of IIH and MS. METHODS: AP -1, TNF-alpha, and IL -6 protein values in the CSF of the cases were evaluated by the ELISA method. The numerical measures of the groups and the ability of AP -1 to distinguish the groups were analyzed with the ROC curve. RESULTS: There was no difference between the groups in CSF TNF-alpha, IL -6, CSF, and serum biochemistry analyses. However, it was determined that the AP -1 concentration (pg/ml) was significantly higher in the IIH group, the sensitivity of AP -1 in separating those with IIH was 75%, and the specificity in separating those with MS was 60% in those with an AP -1 concentration of 606.5 and above. CONCLUSION: According to our results, the fact that CSF TNF-alpha and IL -6 values did not differ in IIH compared to MS revealed that IIH could not methodologically control MS, and AP -1 was a supportive parameter in differentiating both diseases (Tab. 2, Fig. 1, Ref. 31) . Text in PDF www.elis.skArticle Citation Count: 0Ozone Administration Reduces Myocardial Ischemia Reperfusion Injury in Streptozotocin Induced Diabetes Mellitus Rat Model(Dove Medical Press Ltd, 2024) Yığman, Zeynep; Dursun, Ali Doğan; Ozer, Abdullah; Yigman, Zeynep; Dursun, Ali Dogan; Arslan, Mustafa; Oktar, Gursel Levent; Basic SciencesObjective: This study aimed to demonstrate whether ozone has cardioprotective effects on the myocardial ischemia-reperfusion injury (IRI) in rats with streptozotocin(STZ)-induced diabetes. Methods: A total of 38 male Wistar Albino rats were divided into five groups as follows: control group (group C,n=6), n =6), diabetic group (group D,n=6), n =6), diabetic ozone group (group DO,n=6), diabetic-ischemia/reperfusion (group DIR,n=6), n =6), diabetic-ischemia/reperfusion-ozone (group DIRO,n=6). n =6). Six rats died during this period and two died because of surgical complications. A myocardial ischemia-reperfusion model was created using a thoracotomy incision from 4th intercostal space. The LAD was ligated using an 8-0 prolene suture for 30min. Ozone was administered intraperitoneally(1mg/kg) 5min before reperfusion. The reperfusion time was 120 min. At the end of the reperfusion procedure, myocardial tissue histopathological examinations, and serum biochemical analyses were performed. Results: The percentage of TUNEL(+) cardiomyocytes/HPF was significantly higher in the DIR group than in the C, D, and DO groups. Conversely, TUNEL positivity was significantly lower in the DIRO group than in the DIR group. The IRI score was significantly higher in the DIR and DIRO groups than that in the C, D, and DO groups. In contrast, the IRI damage score in the DIRO group was significantly lower than that in the DIR group. Serum MDA levels were significantly higher in the DIR group than in the C, D, and DO groups. Similarly, MDA levels were significantly higher in the DIRO group than in the C and D groups. CAT activity was significantly higher in the DIR group than in the C and D groups. SOD activity was significantly higher in the DIR group than in the C and DO groups. Conclusion: Our study showed that ozone exerts cardioprotective effects in STZ-induced diabetic rats through its antioxidant role against oxidative stress. Both biochemical and histological analyses clearly revealed that ozone has beneficial effects against IRI in the diabetic rat myocardium.Article Chronic Pregabalin Treatment Reduced Anxiety, and Acute Pregabalin Treatment Increased Depression-Like Behaviors in Rats(Bmc, 2024) Dursun, Ali Doğan; Akat, Firat; Dursun, Ali Dogan; Zaloglu, Nezahet; Basic SciencesBackgroundPregabalin is an antiepileptic drug that binds to the alpha-2/delta unit at presynaptic voltage-dependent calcium channels. We aimed to investigate the effect of acute and chronic pregabalin administration on anxiety and depression-like behaviors.MethodsFifty-six male Wistar albino rats were divided into seven groups: control, vehicle, and five different dose groups (5, 10, 30, 60, and 100 mg/kg). Pregabalin was administered for two weeks. Depression-like behaviors were evaluated by Forced swimming test. Anxiety-like behavior (ALB) was evaluated by Open field test (OFT), Elevated Plus Maze (EPM), and light-dark box. Subjects underwent the forced swimming test (FST) after the first dose, while the open field test (OFT), elevated plus maze (EPM), and light-dark box (LDB) were performed after two weeks of treatment. Further sucrose preference test was conducted to evaluate anhedonia until the end of the experiment.ResultsIn the forced swimming test, depression-like behaviors increased after acute single-dose administration of 10, 30, 60, 100 mg/kg pregabalin. According to OFT results, chronic 100 mg/kg pregabalin showed anxiolytic effects by decreasing grooming, and freezing behaviors. In addition, 100 mg/kg chronic pregabalin administration significantly increased the time spent in the central region, the number of entries to the center, and the unsupported rearing number without causing any change in locomotor activity. According to EPM results, both chronic 60 and 100 mg/kg pregabalin treatments showed anxiolytic effects by increasing open arm time and head dipping behavior. In addition, 60 and 100 mg/kg chronic pregabalin administration significantly decreased stretch attend posture. All pregabalin administrations between 5 and 100 mg/kg displayed anxiolytic effects in the LDB. Sucrose preference was above 65% for the duration of all experiments and subjects did not show anhedonia.ConclusionAcute pregabalin treatment triggered depression-like behaviors. Anhedonia, which may be associated with depression, was not observed during chronic treatment. Moreover, chronic treatment with pregabalin revealed potent anxiolytic effects in different behavior patterns and doses for all tests of unconditional anxiety. In particular, 100 mg/kg chronic pregabalin administration decreased anxiety-like behaviors in all experiment setups. Although the anxiolytic effect was demonstrated in chronic treatment, acute treatment of pregabalin induced depression-like behaviors, and thus in clinical practice should be done with caution, especially in patients with anxiety-depression comorbidity.Conference Object Citation Count: 0The Effect of Different Doses Apelin 13 on Erythrocyte Deformability in Rats(Wiley, 2019) Dursun, Ali Doğan; Ozdemir, Cagri; Comu, Faruk Metin; Kucuk, Aysegul; Arslan, Mustafa; Basic Sciences[No Abstract Available]Conference Object Citation Count: 0Investigation of Analgesic Minimum Effective Dose of Apelin-13 With Different Doses of Intraperitoneal Injections and its Effects on Kidney Tissue(Wiley, 2020) Dursun, Ali Doğan; Ozdemir, Cagri; Sezen, Saban; Kucuk, Aysegul; Arslan, Mustafa; Basic Sciences[No Abstract Available]Article Citation Count: 0Enhancement of Paclitaxel Therapeutic Effect by Aptamer Targeted Delivery in Plga Nanoparticles(2021) Dursun, Ali; Dursun, Ali Doğan; Ucak, Samet; Özalp, Veli Cengiz; Poyraz, Fatma Sayan; Yilmaz, Elif; Mansuroglu, Banu; Ozalp, Veli Cengiz; Dursun, Ali Doğan; Özalp, Veli Cengiz; Dursun, Ali Doğan; Özalp, Veli Cengiz; Basic Sciences; Basic SciencesObjectives: Paclitaxel is a drug molecule used in the therapy of various cancer types, including breast cancer. It is one of the preferred chemotherapy agent due to its high efficacy. However, many side effects have been observed associ- ated with paclitaxel use such as allergy, hair loss, diarrhea and pain. Methods: We evaluated therapeutic efficacy of paclitaxel when it is actively targeted to breast cancer tumours inside a polymeric nanoparticle. Targeted delivery of paclitaxel to tumour sites has been reported as an improved cytotoxicity strategy with a variety of nanoparticles. In this study, poly Lactic-co-Glycolic Acid (PLGA) nanoparticles were used as drug carrier and nucleolin aptamers as affinity targeting agents. Results: Paclitaxel molecules were entrapped during the synthesis of PLGA nanoparticles of 238 nm in diameter. The encapsulation and loading efficiencies of paclitaxel was 97% and 21% respectively. The paclitaxel loaded PLGA nanoparticles were functionalized with nucleolin aptamers and their targeting ability to cultured mouse cancer cells was determined for two cell lines (E0771 and 4T1). E0771 cell line was chosen for the preparation of allograph breast cancer mouse models. Evaluations of the targeted paclitaxel in PLGA nanoparticles showed 38% better performance in inhibiting tumour growth compared to free paclitaxel treatment groups of mouse models. Conclusion: The chemotherapeutic effect of cancer drugs like paclitaxel can be increased by loading inside tumour targeted polymeric nanoparticlesArticle Citation Count: 0Serum Asporin Levels in Maintenance Hemodialysis Patients Without Osteoarthritis(Galenos Publishing House, 2023) Dursun, Ali Doğan; Dursun, Ali Doğan; Demir, Canan; Demir, Canan; Dursun, Ali Doğan; Demir, Canan; Dursun, Ali Doğan; Demir, Canan; Basic Sciences; Basic SciencesAims: Several human and experimental studies have shown that small leucine-rich proteoglycans might play a significant role in inflammation and fibrosis in various renal diseases. However, as far as we know, no study has reported asporin levels in patients with advanced renal disease. The primary aim of this study was to determine serum asporin levels in hemodialysis (HD) patients without symptomatic osteoarthritis. Methods: This single-center, cross-sectional study prospectively enrolled maintenance HD patients and healthy control subjects. Subjects with clinically clear osteoarthritis were excluded. Serum asporin level was measured via Human ASPN (Asporin) ELISA Kit (Elabscience Biotechnology Inc. Houston, Texas, USA) in fasting blood samples. Results: The study included 25 (mean age: 43.3±13.5 years, 60% were females) patients and 29 control subjects (mean age: 38.0±8.8 years, 37.9% were females). Patients and controls were similar in age and sex. Serum asporin levels were significantly higher in HD patients compared with the controls 2.4 (0.9-4.8) ng/mL vs. 0.3 (0.2-0.6) ng/mL, respectively, p<0.001). Asporin levels were not correlated with age (r=0.344, p=0.092) and the duration of HD (r=0.385, p=0.077). Among HD patients, asporin level was not significantly correlated with C-reactive protein, parathyroid hormone, calcium, or phosphorus levels. Conclusions: This study showed that serum asporin levels were significantly elevated in patients undergoing HD. Further studies must elucidate the possible origins of increased asporin in these patients.Article Citation Count: 0Investigation of Sars-Cov Antibody Levels After Covid-19 Vaccine in Chronic Hepatitis B Patients(Aepress Sro, 2024) Kinikli, Sami; Dursun, Ali Doğan; Afsar, Fatma Elcin; Dursun, Ali Dogan; Afşar, Fatma Elçin; Aksoy, Altan; Karahan, Gizem; Cesur, Salih; Urtimur, Ufuk; Basic SciencesAIM: The aim was to compare SARS-CoV-2 IgG antibody levels in chronic hepatitis B patients and healthcare personnel selected as the control group and to determine factors such as age, gender, vaccine type, and number of vaccines that may affect the antibody levels. MATERIALS AND METHODS: 87 chronic hepatitis B (CHB) patients followed in Ankara Training and Research Hospital Infectious Diseases Clinic and Mamak State Hospital Infectious Diseases outpatient clinic and 89 healthcare personnel selected as the control group were included in the study. SARS-CoV-2 IgG antibody levels in the serum samples of patients and healthcare personnel who received the COVID-19 vaccine were studied with the ELISA method in the Microbiology Laboratory of Ankara Training and Research Hospital, using a commercial ELISA kit (Abbott, USA) in line with the recommendations of the manufacturer. In the study, SARS-CoV-2 IgG levels were compared in CHB patients and healthcare personnel. In addition, the relationship between SARS-CoV-2 antibody level, gender, average age, natural history of the disease, number of vaccinations, vaccine type (Coronavac TM vaccine alone, BNT162b2 vaccine alone or Coronavac TM and BNT162b2 vaccine (heterologous vaccination)), treatment duration of CHB was investigated. Statistical analyses were made in the SPSS program. A value of p <= 0.05 was considered statistically significant. FINDINGS: A total of 167 people, including 87 CKD patients and 80 healthcare personnel as the control group, were included in the study. SARS-CoV-2 IgG antibody levels were detected above the cut-off level in the entire study group, regardless of the vaccine type. No difference was detected in SARS-CoV-2 IgG titers after COVID-19 vaccination between CHB patients and healthcare personnel. There was a statistically significant difference in SARS-CoV-2 IgG antibody levels among individuals participating in the study according to vaccine types. Compared to those who received Coronavac TM vaccine alone, the average SARS-CoV-2 IgG level was found to be statistically significantly higher in those who received BNT162b2 vaccine alone or heterologous vaccination with Coronavac TM + BNT162b2 vaccine. There was no difference between the groups in terms of age, gender, number of vaccinations, natural transmission of the disease, and duration of antiviral therapy in the CHD patient group. CONCLUSION: As a result, SARS-CoV-2 IgG antibody levels above the cut-off value were achieved with Coronavac TM and BNT162b2 vaccines in both CHD patients and healthy control groups. however, both CHD patients and healthcare personnel had higher antibody levels than those who received BNT162b2 alone or those who received heterologous vaccination had higher antibody levels than those with Coronavac TM alone. Therefore, if there are no contraindications, BNT162b2 vaccine may be preferred in CHB and health personnel (Tab. 2, Ref. 14) .
