Bargh, Saharnaz

Profile URL
https://hdl.handle.net/20.500.14411/11544
Name Variants
Bargh, Saharnaz B., Saharnaz B., S. Bargh, S. Saharnaz, Bargh
Job Title
Dr. Öğr. Üyesi
Email Address
saharnaz.bargh@atilim.edu.tr
Main Affiliation
Medical Laboratory Techniques Program
Status
Current Staff
Website
ORCID ID
0000-0003-0766-8065
Scopus Author ID
59272664500
Turkish CoHE Profile ID
446919
Google Scholar ID
PA4abaUAAAAJ
WoS Researcher ID
QAY-2345-2026
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1

Citations

26

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Scholarly Output

2

Articles

2

Views / Downloads

1/0

Supervised MSc Theses

0

Supervised PhD Theses

0

WoS Citation Count

2

Scopus Citation Count

2

Patents

0

Projects

0

WoS Citations per Publication

1.00

Scopus Citations per Publication

1.00

Open Access Source

1

Supervised Theses

0

JournalCount
Biotechnology and Applied Biochemistry1
International Journal of Radiation Research1
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Scholarly Output Search Results

Now showing 1 - 2 of 2
  • Article
    Citation - WoS: 2
    Citation - Scopus: 2
    Selection of DNA Aptamers Against Parathyroid Hormone for Electrochemical Impedimetric Biosensor System Development
    (John Wiley and Sons Inc, 2025-03-19) Didarian, Reza; Bargh, Saharnaz; Gulerman, Almina; Ozalp, Veli Cengiz; Erel, Ozcan; Yildirim-Tirgil, Nimet
    This work presents the pioneering development of an aptamer-based electrochemical biosensor for real-time monitoring of parathyroid hormone (PTH) levels, with a focus on intraoperative assessment during parathyroid surgery. It introduces, for the first time, the selection and characterization of aptamers targeting distinct segments of the PTH peptide. The study demonstrates the feasibility and efficacy of the biosensing platform through a precisely designed experimental framework, including SELEX-based aptamer selection, aptamer-peptide interaction analysis, and biosensor fabrication. The SELEX process yields aptamers with notable binding affinities to different fragments of PTH, with the PTH (53-84) aptamer showing particularly sensitive binding to the hormone's C terminus, allowing for precise PTH analysis. Electrochemical characterization reveals significant changes in electrochemical impedance spectroscopy (EIS) signals upon exposure to varying PTH concentrations, highlighting the sensor's sensitivity and selectivity. The increase in charge transfer resistance (Rct) values with rising PTH concentrations underscores the biosensor's capability to detect PTH-induced structural changes, validating its potential for accurate measurement. The biosensor shows remarkable selectivity in the presence of common interferents in serum samples, ensuring precise PTH detection. Stability assessments over a 45-day storage period demonstrate the biosensor's robustness and long-term reliability, affirming its practical suitability. In summary, the developed aptamer-based biosensor represents a promising tool for sensitive and selective PTH detection, with potential applications in biomedical research and clinical diagnostics, particularly for intraoperative PTH analysis during parathyroidectomy. Continued research and optimization efforts hold promise for enhancing its performance and expanding its utility in diverse healthcare settings.
  • Article
    Development and In-Vitro Evaluation of Gallium-68 Labelled Staphylococcus Aureus-Specific Aptamer as a Potential PET Agent for Infection Imaging
    (IJRR-Iranian Journal Radiation Res, 2025) Bargh, S.; Ozkul, C.; Timur, S. S.; Ozalp, V. C.; Erdogan, S.
    Background: Staphylococcus aureus (S. aureus) is the most common causative pathogen associated with a wide range of infections, from mild to life-threatening conditions such as osteomyelitis, endocarditis, and pneumonia. Early detection and reliable differentiation between infection and sterile inflammation are essential for accurate diagnosis and effective treatment. However, most radiopharmaceuticals currently available fail to discriminate between these conditions, underscoring the need for infection-specific imaging agents. Materials and Methods: In this study, a Gallium-68 (Ga-68)-labeled S. aureus-specific aptamer was developed as a potential PET infection imaging probe. Aptamers were selected using the cell- systematic evolution of ligands by exponential enrichment (SELEX) method, and their specificity was verified by fluorescence-based binding assays. Radiolabeling was achieved via DOTA chelation, and radiochemical purity was determined. Additionally, in vitro binding assays were performed with S. aureus, while Escherichia coli (E. coli) served as a control. Results: The aptamer exhibited an affinity constant (K-a) of 2260 +/- 634 CFU/ mL and a linear detection range of 250-2x10(4) CFU/mL, with a limit of detection of 171 CFU/mL for S. aureus. The Ga-68-labeled aptamer demonstrated radiochemical purity greater than 99%. In vitro binding increased linearly with rising S. aureus concentrations (10(3)-2x10(4) CFU/mL), while minimal binding to E. coli confirmed its specificity. Conclusion: These results demonstrate that the Ga-68-labeled S. aureusspecific aptamer holds promise as an infection-targeted PET imaging agent. Although currently limited to in vitro evaluation, such aptamer-based radiopharmaceuticals may contribute to improved diagnosis and imaging of infectious diseases.