Browsing by Author "Sahinturk, Varol"

Now showing 1 - 3 of 3

Citation - WoS: 12
Citation - Scopus: 10
Cardiac Hypertrophy Caused by Hyperthyroidism in Rats: the Role of Atf-6 and Trpc1 Channels
(Canadian Science Publishing, 2021) Aykanat, Nuriye Ezgi Bektur; Sahin, Erhan; Kacar, Sedat; Bagci, Ridvan; Karakaya, Serife; Donmez, Dilek Burukoglu; Sahinturk, Varol
Hyperthyroidism influences the development of cardiac hypertrophy. Transient receptor potential canonical channels (TRPCs) and endoplasmic reticulum(ER) stress are regarded as critical pathways in cardiac hypertrophy. Hence, we aimed to identify the TRPCs associated with ER stress in hyperthyroidism-induced cardiac hypertrophy. Twenty adult Wistar albino male rats were used in the study. The control group was fed with standard food and tap water. The group with hyperthyroidism was also fed with standard rat food, along with tap water that contained 12 mg/L of thyroxine (T4) for 4 weeks. At the end of the fourth week, the serum-free triiodothyronine (T3), T4, and thyroid-stimulating hormone (TSH) levels of the groups were measured. The left ventricle of each rat was used for histochemistry, immunohistochemistry, Western blot, total antioxidant capacity (TAC), and total oxidant status (TOS) analysis. As per our results, activating transcription factor 6 (ATF-6), inositol-requiring kinase 1 (IRE-1), and TRPC1, which play a significant role in cardiac hypertrophy caused by hyperthyroidism, showed increased activation. Moreover, TOS and serum-free T3 levels increased, while TAC and TSH levels decreased. With the help of the literature review in our study, we could, for the first time, indicate that the increased activation of ATF-6, IRE-1, and TRPC1-induced deterioration of the Ca2+ ion balance leads to hypertrophy in hyperthyroidism due to heart failure.
Effect of 25(OH)D Supplementation on Adipokines and Advanced Glycation End Products in Experimental Type 2 Diabetic and Obesity Rat Model
(Univ Karachi, 2025) Toprak, Cigdem; Yigitaslan, Semra; Aykanat, Ezgi Bektur; Sirmagul, Basar; Sahinturk, Varol
Diabetes mellitus is a global health problem and vitamin D deficiency is thougt to be a reasonal factor for development of diabetes. Our aim is to investigate among vitamin D levels and blood glucose, insulin resistance, adiponectin, apelin, tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), oxidative stress index (OSI) levels and advanced glycation end products (AGEs) formation in modeled obesity and type 2 diabetes mellitus (T2DM) rats. During the 14-week experiment; 1-Control group was fed standart diet-(12 % of calories of fat), 2-Diabetes group was fed high fat diet (HFD)-at the 4th week Streptozotocin (STZ) (35 mg/kg) was injected, 3-Diabetes/vitamin D group was fed HFD-at the 4th week STZ was injected-between 4th-14th week vitamin D supplement was administered, 4-Obesity group was fed HFD-(40% of calories as fat), 5-Obesity/vitamin D group was fed HFD-between 4th-14th week vitamin D supplement was administered. At 14th week, biochemical parameters, adipokine concentrations, kidney tissue cytokine levels, liver tissue oxidative stress index parameters were measured in serum and tissue using commercial ELISA kits, H&E (hematoxylin & Eosin) staining of kidney, heart, liver, coronary artery, renal artery tissues and immunohistochemical staining of kidney tissues were performed. It was concluded that vitamin D can alleviate glucose parameters and lipid parameters in type 2 diabetic and obesity modeled rats and we think it may be useful adjuvant agent in obesity and T2DM treatment.
Citation - Scopus: 8
Effects of Astaxanthin on Metastasis Suppressors in Ductal Carcinoma. a Preliminary Study
(Edizioni Luigi Pozzi, 2021) Badak, Bartu; Aykanat, Nuriye Ezgi Bektur; Kacar, Sedat; Sahinturk, Varol; Arik, Deniz; Canaz, Funda; Basic Sciences
BACKGROUND: Breast cancer (BC) is a major public health problem diagnosed in more than 2 million women worldwide in 2018, causing more than 600,000 deaths. 90% of deaths due to breast cancer are caused by metastasis. Metastasis is a complex process that is divided into several steps, including separation of tumor cells from the primary tumor, invasion, cell migration, intravasation, vasculature survival, extravasation, and colonization of the secondary site. Astaxanthin (AXT) is a marine-based ketocarotenoid that has many different potential functions such as anti-oxidant, anti-inflammatory and oxidative stress-reducing properties to potentially reduce the incidence of cancer or inhibit the expansion of tumor cells. This study aims to investigate the effects of astaxanthin as a new metastasis inhibitor on T47D human invasive ductal carcinoma breast cancer cell. MATERIAL AND METHODS: To investigate the effects of the astaxanthin as a new metastasis inhibitor on T47D cell, expression levels of anti-maspin, anti-Kail, anti-BRMS1, and anti-MKK4 were examined by western blot. Also, we evaluated differences of these suppressors expression levels in tissue sections of 10 patients diagnosed with in situ and invasive ductal carcinoma by immunohistochemistry method. RESULT: 250 mu M astaxanthin increased the activation of all metastasis suppressing proteins. Also, these metastasis suppressors showed higher expression in invasive ductal carcinoma tissues than in situ ductal carcinoma patients. CONCLUSION: We think that astaxanthin is a promising therapeutic agent for invasive ductal carcinoma patients. The effects of astaxanthin on metastasis in breast cancer should be investigated further based on these results.