Browsing by Author "Küçük, Ayşegül"

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Farklı Dozlarda Uygulanan Apelin-13’ün Analjezik Minimum Etkin Dozu ve Böbrek Dokusu Üzerine Etkisi: Deneysel Çalışma
(2024) Küçük, Ayşegül; Dursun, Alı Dogan; Arslan, Mustafa; Ozdemir, Cagri; Sezen, Şaban Cem; 01. Atılım University
Amaç: Apelin ve APJ sinyal yolu; kalp, böbrek ve akciğer dâhil çeşitli dokularda eksprese edilmektedir. Bu yol kan bas ıncı, kardiyak kontraktilite, kalp h ızı, nosisepsiyon, apoptozis ve inflamasyon üzerinde çeşitli etkilere sahiptir. Mekanizması hâlen anlaşılamamasına rağmen Apelin-13’ün analjezik etkinli ği gösterilmi ştir. Gelecekte koruyucu ve tedavi edici bir ajan olarak kullan ılabilecek olması insan sağlığına önemli katk ılar sağlayacaktır. Bu sebeple ratlarda Apelin- 13’ün minimum analjezik etkin dozunu ve böbrek üzerine etkilerini araştırmayı amaçladık. Gereç ve Yöntemler: 30 adet Wistar Albino erkek rat, randomize olarak 5 gruba ayrıldı. Ratlar kontrol, Apelin-25, Apelin-50, Apelin-100 ve Apelin-200 gruplar ı olarak isimlendirildi. Apelin-13 intraperitoneal olarak 25, 50, 100 ve 200 μg/kg uyguland ı. Kontrol grubuna intraperitoneal olarak ayn ı hacimde salin uygulandı. Apelin uygulanmasından sonra 30. dk, 1. ve 2. saatlerde Hot Plate testi ile analjezik etkinlik de ğerlendirildi. 24 saat sonra histolojik ve biyokimyasal değerlendirmeler için tüm ratlardan kan ve doku örnekleri alındı. Bulgular: Hot Plate sonuçlarına bakıldığında 50 μg/kg ve üzeri Apelin-13’ün analjezik etkinlik gösterdiği tespit edildi. 25 μg/kg dozda analjezik etki görülmedi. 100 ve 200 μg/kg Apelin-13 uygulanan ratların böbrek dokusunda vasküler vakuolizasyon ve hipertrofi, Bowman aralık dilatasyonu ve tübüler hücre dökülmesi anlamlı olarak artmış bulundu. 200 μg/kg Apelin-13 uygulanan ratlarda doku oksidatif stres belirteçleri daha yüksekti. Sonuç: Literatürde Apelin-13’ün farklı dozlarda analjezik etkileri ile ilgili çalışmalara rastlamadık. 200 μg/kg Apelin-13 uygulamas ının böbrek dokusunu olumsuz etkiledi ğini bulduk. Apelin-13’ün minimum analjezik etkin dozunun intraperitoneal olarak uygulanan 50 μg/kg olduğunu tespit ettik.
Potential Protective Effects of Boldine in Rat With an Experimental Myocardial Ischemia-Reperfusion Model
(2025) Küçük, Ayşegül; Dursun, Alı Dogan; Arslan, Mustafa; Sezen, Şaban Cem; Yıldırım, Alperen Kutay; Özer, Abdullah; Yığman, Zeynep; Basic Sciences; 08. Medical School; 01. Atılım University
Objectives: Myocardial ischemia-reperfusion injury (MIRI) remains a major challenge in cardiovascular medicine due to its complex pathophysiology involving oxidative stress, inflammation, and cellular dysfunction. Boldine, a potent natural alkaloid with antioxidant and anti-inflammatory properties, has demonstrated protective effects in various pathological conditions. However, its potential cardioprotective effects in MIRI remain largely unexplored. This study aims to evaluate the protective effects of Boldine in a rat model of MIRI by assessing oxidative stress markers, histopathological changes, and inflammatory responses. Materials and Methods: Male Albino Wistar rats were randomly assigned to four groups: Control, Boldine, myocardial ischemia-reperfusion (MIR), and myocardial ischemia-reperfusion + Boldine (MIR+B). Myocardial ischemia was induced by ligating the left anterior descending coronary artery for 30 minutes, followed by 120 minutes of reperfusion. Boldine (50 mg/kg) was administered intraperitoneally at the onset of reperfusion. Cardiac tissue samples were collected for histopathological evaluation and biochemical analysis, including total antioxidant status (TAS), total oxidant status (TOS), and Oxidative Stress index (OSI). Results: Histopathological analysis revealed significant myocardial disorganization and inflammation in the MIR group compared to controls (p=0.05). Boldine treatment significantly reduced inflammation and myocardial disorganization in the MIR+B group (p<0.05), suggesting a protective effect. Biochemical analysis showed a marked decrease in TAS levels and an increase in TOS and OSI in the MIR group (p<0.001). However, Boldine administration significantly restored TAS levels and reduced TOS and OSI in the MIR+B group (p< 0.001), indicating attenuation of oxidative stress. Conclusion: Boldine exhibits significant cardioprotective effects in a rat model of MIRI by reducing oxidative stress, mitigating myocardial disorganization, and alleviating inflammation. These findings suggest that Boldine may serve as a therapeutic agent in ischemic heart disease. Further research is warranted to elucidate its precise mechanisms of action and potential clinical applications.
Citation - Scopus: 1
Protective Effects of Metformin in Non-Diabetic Rats With Experimentally Induced Lower Extremity Ischemia-Reperfusion Injury
(Turkish National Vascular and Endovascular Surgery Society, 2025) Küçük, Ayşegül; Dursun, Alı Dogan; Arslan, Mustafa; Sezen, Şaban Cem; Yıldırım, Alperen Kutay; Özer, Abdullah; Demirtas, Huseyin; 01. Atılım University
Aim: Lower extremity ischemia-reperfusion (IR) injury can lead to substantial skeletal muscle damage and systemic complications, primarily driven by oxidative stress and inflammation. In addition to its well-known glucose-lowering effects, metformin possesses antioxidant and anti-inflammatory properties that may confer protection against tissue damage caused by IR. This study aims to evaluate the potential protective effects of metformin on skeletal muscle injury using a rat model of lower extremity IR.Material and Methods: A total of twenty-four male Wistar albino rats were randomly divided into four experimental groups: Control (C), Ischemia-Reperfusion (IR), IR with metformin at 4 mg/kg (IR+M4), and IR with metformin at 8 mg/kg (IR+M8). Ischemia was induced by clamping the infrarenal aorta for 45 minutes, followed by a reperfusion period of 120 minutes. In the treatment groups, metformin was administered intraperitoneally at the onset of ischemia. Gastrocnemius muscle tissues were harvested for subsequent histopathological and biochemical evaluations, including measurements of Total Antioxidant Status (TAS), Total Oxidant Status (TOS), and Oxidative Stress Index (OSI).Results: Histopathological analysis demonstrated a significant reduction in muscle atrophy, degeneration, leukocyte infiltration, and fiber fragmentation in the IR+M8 group compared to the IR group. Biochemical assessments showed that TAS levels were considerably elevated, whereas TOS and OSI levels were markedly reduced in the metformin-treated groups, with the most prominent effects observed at the higher dosage of 8 mg/kg.Conclusion: The findings indicate that metformin exerts a dose-dependent protective effect against skeletal muscle injury resulting from lower extremity ischemia-reperfusion in rats. These protective properties are likely due to metformin’s antioxidant and anti-inflammatory mechanisms, highlighting its potential therapeutic value in mitigating IR-induced tissue damage.