Effect of 25(Oh)d Supplementation on Adipokines and Advanced Glycation End Products in Experimental Type 2 Diabetic and Obesity Rat Model

Abstract

Diabetes mellitus is a global health problem and vitamin D deficiency is thougt to be a reasonal factor for development of diabetes. Our aim is to investigate among vitamin D levels and blood glucose, insulin resistance, adiponectin, apelin, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), oxidative stress index (OSI) levels and advanced glycation end products (AGEs) formation in modeled obesity and type 2 diabetes mellitus (T2DM) rats. During the 14-week experiment; 1-Control group was fed standart diet-(12 % of calories of fat), 2-Diabetes group was fed high fat diet (HFD)-at the 4th week Streptozotocin (STZ) (35 mg/kg) was injected, 3-Diabetes/vitamin D group was fed HFD-at the 4th week STZ was injected-between 4th-14th week vitamin D supplement was administered, 4-Obesity group was fed HFD-(40% of calories as fat), 5-Obesity/vitamin D group was fed HFD-between 4th-14th week vitamin D supplement was administered. At 14th week, biochemical parameters, adipokine concentrations, kidney tissue cytokine levels, liver tissue oxidative stress index parameters were measured in serum and tissue using commercial ELISA kits, H&E (hematoxylin & Eosin) staining of kidney, heart, liver, coronary artery, renal artery tissues and immunohistochemical staining of kidney tissues were performed. It was concluded that vitamin D can alleviate glucose parameters and lipid parameters in type 2 diabetic and obesity modeled rats and we think it may be useful adjuvant agent in obesity and T2DM treatment. © 2025 Pakistan Journal of Pharmaceutical Sciences. All rights reserved.