Diğer Yayınlar

Permanent URI for this collectionhttps://hdl.handle.net/20.500.14411/27

Browse

Browsing Diğer Yayınlar by WoS Q "Q2"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Article
    Fluorescent and Electrochemical Detection of Nuclease Activity Associated With Streptococcus Pneumoniae Using Specific Oligonucleotide Probes
    (Analyst, 2024) Goikoetxea, Garazi; Akhtar, Khadija-tul Kubra; Prysiazhniuk, Alona; Borsa, Barış A.; Aldağ, Mehmet Ersoy; Kavruk, Murat; Özalp, Veli Cengiz; Hernandez, Frank J.
    Streptococcus pneumoniae (S. pneumoniae) represents a significant pathogenic threat, often responsible for community-acquired pneumonia with potentially life-threatening consequences if left untreated. This underscores the pressing clinical need for rapid and accurate detection of this harmful bacteria. In this study, we report the screening and discovery of a novel biomarker for S. pneumoniae detection. We used S. pneumoniae nucleases as biomarker and we have identified a specific oligonucleotide that works as substrate. This biomarker relies on a specific nuclease activity found on the bacterial membrane, forming the basis for the development of both fluorescence and electrochemical biosensors. We observed an exceptionally high sensitivity in the performance of the electrochemical biosensor, detecting as low as 102 CFU mL−1, whereas the fluorescence sensor demonstrated comparatively lower efficiency, with a detection limit of 106 CFU mL−1. Moreover, the specificity studies have demonstrated the biosensors’ remarkable capacity to identify S. pneumoniae from other pathogenic bacteria. Significantly, both biosensors have demonstrated the ability to identify S. pneumoniae cultured from clinical samples, providing compelling evidence of the potential clinical utility of this innovative detection system.
  • Thumbnail Image
    Article
    The Effect of Kangaroo Care on Paternal Attachment a Randomized Controlled Study
    (Advances in Neonatal Care, 2023) Yıldırım, Fatma; Büyükkayacı Duman, Hayriye; Şahin, Ebru; Vural, Gülşen
    Background: During the first interaction between the father and the infant, touch can be very important especially father–infant skin-to-skin contact. Few studies have focused on the effect of kangaroo care (KC) on paternal attachment. Purpose: This randomized controlled study was conducted to determine the effect of KC on paternal attachment. Methods: A total of 90 fathers of healthy newborns, including 45 in the intervention group and 45 in the control group who met the inclusion criteria, were included randomly in the study. Data were collected using the Introductory Information Form at study admission and the Father–Infant Attachment Scale (FIAS) at 3 months of age. T test, Mann–Whitney U test, and Kruskal–Wallis test were used for statistical analysis. Results: The mean FIAS scores for the intervention group (I) were higher than for the control group (C) (I: 80.57 ± 13.70; C: 56.76 ± 13.23) (P < .05). Patience and tolerance (I: 13.70 ± 1.18; C: 11.57 ± 2.30), pleasure in interaction (I: 29.50 ± 2.86; C: 17.13 ± 5.93), and love and pride (I: 37.37 ± 2.85; C: 28.06 ± 5.82) mean scores for FIAS subdimensions in the intervention group were also higher than in the control group (P < .05). Implications for Practice and Research: Findings of this study demonstrate that KC has the potential to increase paternal attachment. Healthcare providers should provide discharge education for fathers on KC to increase father–infant attachment. There is a need for studies with larger samples in different cultures on the factors related to parents that affect father–infant attachment and evidence-based practices that increase attachment.