Browsing by Author "İşgör,Y.G."

Now showing 1 - 2 of 2

Citation - Scopus: 1
Computer Vision Based Automated Cell Counting Pipeline: a Case Study for Hl60 Cancer Cell on Hemocytometer
(Scientific Publishers of India, 2018) Özkan,A.; İşgör,S.B.; Şengül,G.; İşgör,Y.G.; Department of Electrical & Electronics Engineering; Computer Engineering; Chemical Engineering; 15. Graduate School of Natural and Applied Sciences; 06. School Of Engineering; 01. Atılım University
Counting of cells can give useful information about the cell density to understand the concerning cell culture condition. Usually, cell counting can be achieved manually with the help of the microscope and hemocytometer by the domain experts. The main drawback of the manual counting procedure is that the reliability highly depends on the experience and concentration of the examiners. Therefore, computer vision based automated cell counting is an essential tool to improve the accuracy. Although the commercial automated cell counting systems are available in the literature, their high cost limits their broader usage. In this study, we present a cell counting pipeline for light microscope images based on hemocytometer that can be easily adapted to the various cell types. The proposed method is robust to adverse image and cell culture conditions such as cell shape deformations, lightning conditions and brightness differences. In addition, we collect a novel human promyelocytic leukemia (HL60) cancer cell dataset to test our pipeline. The experimental results are presented in three measures: recall, precision and F-measure. The method reaches up to 98%, 92%, and 95% based on these three measures respectively by combining Support Vector Machine (SVM) and Histogram of Oriented Gradient (HOG). © 2018, Scientific Publishers of India. All rights reserved.
Citation - Scopus: 9
The Effects of Eryngium Campestre Extracts on Glutathione-S Glutathione Peroxidase and Catalase Enzyme Activities;
(Turkish Pharmacists Association, 2014) Güneş,M.G.; İşgör,B.S.; İşgör,Y.G.; Shomali Moghaddam,N.; Geven,F.; Yildirim,Ö.; 01. Atılım University
Eryngium campestre L. (Apiaceae) are known in Turkish folk medicine as ‘Bogadikeni’ and wildly distributed in all parts of Turkey. Infusion of different parts of this species are well known as diuretic, appetizer and stimulant. In the present study, methanol extracts from flowers and leaves of E. campestre were evaluated for their potential medicinal value in terms of biological targets participate in antioxidant defense such as glutathione-S-transferase (GST), glutathione peroxidase (GPx) and catalase (CAT). Results obtained show that the methanol extract of flowers were found to have higher amount of phenolic and flavonoid contents than the leaves. The evaluation of extracts against biological target revealed that, the maximum GST enzymes inhibition activities was observed with methanol extract from flowers of E. campestre with IC50 value of 363 ng/mL. In this study, none of the extracts evaluated were shown any reasonable GPx and CAT inhibition. © 2014, Turkish Pharmacists Association. All rights reserved.