Tevlek,A.2024-09-102024-09-10202400079-610710.1016/j.pbiomolbio.2024.08.0022-s2.0-85201364525https://doi.org/10.1016/j.pbiomolbio.2024.08.002https://hdl.handle.net/20.500.14411/7380In the bloodstream or other physiological fluids, “circulating cells and sub-cellular bio-particles” include many microscopic biological elements such as circulating tumor cells (CTCs), cell-free DNA (cfDNA), exosomes, microRNAs, platelets, immune cells, and proteins are the most well-known and investigated. These structures are crucial biomarkers in healthcare and medical research for the early detection of cancer and other disorders, enabling treatment to commence before the onset of clinical symptoms and enhancing the efficacy of treatments. As the size of these biomarkers to be detected decreases and their numbers in body fluids diminishes, the detection materials, ranging from visual inspection to advanced microscopy techniques, begin to become smaller, more sensitive, faster, and more effective, thanks to developing nanotechnology. This review first defines the circulating cells and subcellular bio-particles with their biological, physical, and mechanical properties and second focuses on their diagnostic importance, including their most recent applications as biomarkers, the biosensors that are utilized to detect them, the present obstacles that must be surmounted, and prospective developments in the domain. As technology advances and biomolecular pathways are deepens, diagnostic tests will become more sensitive, specific, and thorough. Finally, integrating recent advances in the diagnostic use of circulating cells and bioparticles into clinical practice is promising for precision medicine and patient outcomes. © 2024 Elsevier Ltdeninfo:eu-repo/semantics/closedAccessBio-particlesBiomarkersBiosensorsBody fluidsCTCsDetectionDiagnosisReviewQ21921936