Dursun, Ali DoğanKucuk, AysegulPolat, YucelKilicarslan, AydanSungu, NuranKartal, HakanDursun, Ali DoganArslan, MustafaBasic Sciences2024-07-052024-07-05202191177-888110.2147/DDDT.S2793182-s2.0-85101119632https://doi.org/10.2147/DDDT.S279318https://hdl.handle.net/20.500.14411/1937Küçük, Ayşegül/0000-0001-9316-9574; Arslan, Mustafa/0000-0003-4882-5063; Dursun, Ali Dogan/0000-0001-9056-0025; kilicarslan, aydan/0000-0002-7981-4458; Polat, Yucel/0000-0002-3733-7198Aim: The aim of this study was to evaluate the effects of irisin in a murine model of hind limb ischemia reperfusion (I/R). Methods: The mice were divided into four groups (n = 6 in each group): control, irisin, ischemia reperfusion (I/R), and irisin-ischemia reperfusion (I-I/R). Irisin (0.5 mu g.g(-1), intraperitoneally [i.p.]) was administered 30 min before the I/R procedure. After 2 h of ischemia and 2.5 h of reperfusion, blood and tissue samples were taken for biochemical and histopathological analysis. The results were analyzed by Kruskal-Wallis and Mann-Whitney U-tests. Results: There was a statistically significant difference in the total antioxidant status (TAS) and total oxidant status (TOS) levels in all the groups. The TAS level in the I/R group was significantly lower than that in the control, irisin, and I-I/R groups, whereas the TOS level was significantly higher in the I/R group as compared with that in the other groups. Caspase3 activity and caspase-8 activity, indicators of inflammation, were significantly higher in the I/R and I-I/R groups as compared with those in the control and irisin groups. Conclusion: Irisin may have protective effects in skeletal muscle ischemia reperfusion injury.eninfo:eu-repo/semantics/openAccessirisinischemia reperfusioncaspase-3caspase-8miceArticleQ1Q115361368WOS:00061633640000133574655