Ucak, SametYegin, ZeynepYurt, Mediha Nur ZaferSudagidan, MertAltunbas, OsmanOzalp, Veli Cengiz2025-03-052025-03-0520251612-18721612-188010.1002/cbdv.202403049https://doi.org/10.1002/cbdv.202403049https://hdl.handle.net/20.500.14411/10463Yurt, Mediha Nur Zafer/0000-0002-3064-3811; Ucak, Samet/0000-0002-3461-2481Microorganisms linked to the grapevine, Vitis vinifera, can impact crop quality, plant growth, and human and plant health. This study examined the bacterial community structures of brined grapevine leaves (n = 56) taken from seven distinct regions in T & uuml;rkiye using next-generation sequencing technology. Investigations were also conducted into the samples' chemical properties. Firmicutes was the dominant phylum, followed by Proteobacteria, Actinobacteria, and Bacteroidetes. Bacillaceae was the predominant family in the analyzed samples, followed by Clostridiaceae, Peptostreptococcaceae, and Enterobacteriaceae. Bacillus was the dominant genus, followed by Lysinibacillus and Clostridium sensu stricto. The samples exhibited differences in terms of pH, total acidity, and salt content. The pH values of the brined grapevine leaves ranged from 2.31 to 6.91, the acidity levels ranged from 0.09% to 1.80%, and the salt percentages ranged from 3.39% to 49.14%. This research provides pioneering information for bacterial community analysis of brined grapevine leaves.eninfo:eu-repo/semantics/closedAccess16S Rrna | Bacterial Community Structure | Brined Grapevine Leaf | Metagenomic Analysis | <Italic>Vitis Vinifera</Italic>ArticleQ3Q4WOS:00142187520000139925312