Dursun, Ali DoğanDursun, Ali DoganDogan, SonerKavruk, MuratKavruk, MuratTasbasi, B. BusraSudagidan, MertYilmaz, M. DenizTuna, Bilge G.Basic SciencesNutrition and Dietetics2024-07-052024-07-05202220003-26541364-552810.1039/d1an02332b2-s2.0-85128126647https://doi.org/10.1039/d1an02332bhttps://hdl.handle.net/20.500.14411/1862Kavruk, Murat/0000-0001-5331-7253; Dursun, Ali Dogan/0000-0001-9056-0025; Tuna, Bilge Guvenc/0000-0003-1348-1336; Yilmaz, Bayram/0000-0002-2674-6535; Özalp, Veli Cengiz/0000-0002-7659-5990; Yilmaz, Mahmut/0000-0001-5793-0805Intercellular Adhesion Molecule-1 (ICAM-1) is considered to be a cancer biomarker in the assessment of metastatic potential in patients and an early indicator of atherosclerosis. A labelless biosensor based on the surface plasmon resonance (SPR) signal from the specific affinity interaction of an aptamer and a soluble ICAM-1 protein was developed for blood samples. The developed aptasensor provided real-time information on the concentration of the ICAM-1 protein in blood when integrated to a purification step based on a magnetic pull-down separation. The SPR aptasensor was highly specific with a limit of detection of 1.4/0.2 ng ml(-1), which was achieved through aptamer-functionalized silica-coated magnetic nanoparticles.eninfo:eu-repo/semantics/closedAccess[No Keyword Available]ArticleQ2147816631668WOS:00077108800000135312740