Gokce, EvrimAkat, FiratDursun, Ali DoganGunes, EmelBayram, PinarBillur, DenizKoc, EmineBasic Sciences2024-10-062024-10-06201911108-7161[WOS-DOI-BELIRLENECEK-71]https://hdl.handle.net/20.500.14411/8877DURSUN, ALI DOGAN/0000-0001-9056-0025; BILLUR, DENIZ/0000-0001-8541-8251; Gunes, Emel/0000-0003-3599-5151; AKAT, Firat/0000-0003-1609-6847; Bayram, Pinar/0000-0001-9924-7051; Gokce, Evrim/0000-0003-1548-8785Objectives: Eccentric contraction occurs when the muscle lengthens under tension. Damage-induced responses seen in the muscle after eccentric exercise usually experienced by sedentary individuals. This study aims to investigate muscle damage on different slopes. Methods: 32 male Wistar albino rats randomly divided into four groups: sedentary, horizontal running, and eccentric exercise (-8 degrees, -16 degrees) groups. Animals ran for 90 min with the speed of 25 m/s for five days. After 48h from the last exercise, rats were sacrificed, and plasma creatine kinase (CK), heat shock protein 70 (HSP70) levels were examined. Plasma and soleus total oxidant/antioxidant status (TOS-TAS) and histological changes of soleus muscle assessed. Results: CK and HSP70 significantly increased in 16 degrees EE group. TOS increased at 16 degrees EE and 8 degrees EE, but oxidative stress index (OSI) was only high at 8 degrees EE group. Mononuclear cell infiltration and the angiogenesis increased in soleus after eccentric exercise, and there was a correlation with slope. Sarcomere breaks were detected in 16 degrees EE group also in a correlation with slope. Conclusions: Consequently, sedentary individuals are vulnerable to injuries induced by eccentric contraction. Therefore, our study provides information for reconsidering rehabilitation and training programs.eninfo:eu-repo/semantics/closedAccessCreatine KinaseEccentric ExerciseHSP70Muscular DamageSmall Animal TreadmillArticleQ4Q3194412421WOS:00050025730000431789292